pTP (AC_000019) Virus Tagged ORF Clone

CAT#: VC100079

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 35], codon optimized for human cell expression, AP_000577



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CNY 7,890.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100079 represents NCBI reference of AP_000577 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTTTCTATTAATGACTGCGCCAGACTGACCGGGCAGACTGTGCCTACCATGAATTATTTCATGC
CTCTTCGCAATATATGGAACCGAGTTCGGGAATTCCCCAGAGCTTCAACGACCGCAGCAGGAATTACATG
GATGTCAAGATACATATATGGCTACCATCGCCTGATGTTGGAGGATCTGGCGCCAGGCGCGCCTGCCACC
GAAAGATGGCCCCTGTATCGACAGCCCCCCCCTCACTTCCTTATTGGGTATCAGTACCTTGTGCGCACCT
GCAATGACTACATCTTCGACACCCGGGCCTACAGTAGACTCAAATACACAGAACTGGTGAGACCTGGTCA
CCAAACAGTAAATTGGTCCGTAATGGCCAATTGCGCTTACACCATAAATACGGGTGCCTACCACAGGTTC
GTTGACTTTGACGATTTTCAAGCCACGTTGACACAAGTGCAGCAAGCCATATTGGCAGAACGGGTCGTGG
CCGACTTGGCCTTGGTTCAGCCTATGCGGGGGTTCGGCCTGACTAGAATGCATGGCCGGACTGGAGAGGA
AGAAGTACCTGTAGAACGATTGATGCAGGATTACTACAAGGACCTGGCCCGATGCCAGGACAATGCTTGG
GGGATGGCAAACAGGCTGCGGATCCAGCAAGCCGGACCTAAGGATCTTGTGCTGCTGGCTACAATTCGGA
GATTGAGGACTGCATACTTTAACTTTATAACTTCCTCCATCGTATCTCCATCACGCGACGAAGAGGAAAC
TGAGGACCCCGACAGGACCCATAACCATCCCAGGCCACAGGAAGCAGTTCTGTCCCTGCCATGTGATTGT
GATTGGCTGGATGCTTTCGTGGAGCGATTCAGTGATCCAGTTGATCTTGATACAATCCGAAGTTTGCGGG
CCGTACCTACCGGCCAGCTGATTAAGTGCATAATTTCAGCCCTGAGCCTGCCCAACGGAGAGCCGCCATC
TCACCACTTTAAGGAGATGCGGGGTGGCGTATTTACGCTTCGGCCCCGCGAGAATGGAAGGGCTGTGACT
GAGACCATGAGGCGCAGGAGGGGGGAGGTCATAGAGCGCTTCATAGACAGGCTCCCAGTTCGAAGGAGAA
GACGGCGGGTCCCCCCTCCACCTCCTCCAGAGGATGAGGAGGAAATGCTCGTAGAGGAAGAGATCGAGGA
GGAGGAGGAAATACCTGGGGCATTCGAAAGAGAAGTGCGCGCAACTATTGCCGAGCTGATCCGGCTGCTC
GAAGAGGAGCTCACCGTCTCCGCTCGCAACAGTCAGTTTTTTAATTTTGCTGTAGATTTTTATGAGGCTA
TGGAACGGCTGGAGGCACTGGGCGACGTGTCAGAGATGCCACTGAGGAGGTGGATCATGTACTTTTTCGT
AGCAGAACACATTGCCACAACGCTCAATTATCTTTTTCAACGGCTTTGCAATTACGCAGTATTTACGCGC
CACGTTGAACTGAATTTGGCGCAAGTTGTGATGCGAGCCAGGGACTCAGAAGGCGCTGTAGTTTATAGCC
GAGTCTGGAACGAGGCCGGTATGAATGCCTTTTCCCAGCTTATGGGCAGAATCTCCAACGACCTGGCAGC
CACCGTGGAGCGCGCAGGTCGAGGTGACCTGCAGGAAGAGGAAATAGAACAGTTCATGGCCGAGATAGCT
TATCAGGACAACTCCGGCGATGTCCAGGAAATCCTCAGGCAGGCTGCCGTCAACGATACAGAGATCGACT
CCGTGGAACTGAGTTTTCGCTTCAAACTGACTGGTCCTGTCGCCTTTACTCAGAGGCGACAGATCCAAGA
CGTAAACAGGAGGGTGGTGGCGCACGCCAGTCTGCTGCGGGCTCAGTATCAGAACCTTCCCGTTCGCGGA
GCCGATGTCCCTCTCCCTGCCATGCCCCCTGGGCCGGAGCAACCCTTGCCACCAGGCGCTCGCCCCAGGC
ACCGCTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100079 representing AP_000577
Red=Cloning sites Green=Tags

MALSINDCARLTGQTVPTMNYFMPLRNIWNRVREFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDTRAYSRLKYTELVRPGHQTVNWSVMANCAYTINTGAYHRF
VDFDDFQATLTQVQQAILAERVVADLALVQPMRGFGLTRMHGRTGEEEVPVERLMQDYYKDLARCQDNAW
GMANRLRIQQAGPKDLVLLATIRRLRTAYFNFITSSIVSPSRDEEETEDPDRTHNHPRPQEAVLSLPCDC
DWLDAFVERFSDPVDLDTIRSLRAVPTGQLIKCIISALSLPNGEPPSHHFKEMRGGVFTLRPRENGRAVT
ETMRRRRGEVIERFIDRLPVRRRRRRVPPPPPPEDEEEMLVEEEIEEEEEIPGAFEREVRATIAELIRLL
EEELTVSARNSQFFNFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVAEHIATTLNYLFQRLCNYAVFTR
HVELNLAQVVMRARDSEGAVVYSRVWNEAGMNAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMAEIA
YQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPVRG
ADVPLPAMPPGPEQPLPPGARPRHRF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000019
ORF Size 1968 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000019.1, AP_000577
RefSeq ORF 1968 bp
MW 75.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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