100K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100090

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 35], codon optimized for human cell expression, AP_000588



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CNY 12,350.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100090 represents NCBI reference of AP_000588 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACTCAGCCCTTGTTGAGCACCAGCCCCCGGGTACCCTCACACCTGGTACTTAGCGATGAAGAAA
AGGAACAGTCTCTCTCAATCCCCCCACCCAGTCCCGCTACCACTTCTACTCTGGAGGACAAGGAAGTGGA
TGCCTCTCACGATATGCAGAATAAAAAAGCCAAAGAGTCAGAGACCGATATTGAACAGGACCCAGGATAC
GTAACCCCGGTGGAGCATGAGGAAGAGCTGAAAAGATTCCTGGAACGGGAGGACGAAAATTGTCCCAAAC
AGAGAGCCGATAACTACCACCAAGACGCTGGTAACAGAGATCAGAATACGGATTACTTGATCGGGCTGGA
TGGAGAAGACGCCCTCTTGAAACACCTGGCTAGGCAGTCTCTGATCGTTAAGGATGCCTTGCTTGACCGA
ACCGAAGTGCCGATTAGTGTTGAGGAGCTCTCCTGTGCCTACGAGCTGAATCTGTTTTCACCAAGGACAC
CCCCCAAGAGACAGCCCAATGGGACCTGCGAACCGAATCCCAGGCTCAACTTTTACCCCGCATTCGCTGT
TCCAGAGGTCCTCGCAACGTACCACATCTTTTTTAAAAATCAGAAGATCCCTGTGAGTTGTAGGGCTAAC
AGAACCCGCGCGGATGCTCTGCTTAATCTGGGTCCAGGGTCTAGGCTGCCGGATATTGCCTCTCTGGAGG
AGGTGCCTAAGATTTTTGAAGGTCTCGGTAATAACGAGACAAGAGCAGCTAATGCCTTGCAGAAGGGCGA
AAACGGCATGGATGAGCACCACAGTGTGCTGGTTGAACTGGAAGGAGACAACGCAAGACTGGCAGTGCTC
AAAAGGTCAGTAGAGGTCACACACTTCGCCTATCCTGCAGTCAATCTTCCTCCCAAGGTGATGACAGCCG
TCATGGACCAGTTGTTGATTAAGCGCGCATCACCACTCAGCGAAGATATGCATGATCCGGACGCCTGTGA
CGAAGGCAAGCCAGTCGTGAGCGATGAGCAGCTGACACGGTGGCTGGGAACCGATTCTCCACGCGACCTG
GAAGAACGGCGCAAACTCATGATGGCAGTAGTGCTTGTCACCGTGGAACTGGAGTGCCTGAGGCGATTTT
TCACCGACTCCGAGACCTTGCGCAAACTGGAAGAGAATCTTCACTACACCTTTAGACACGGATTTGTCCG
ACAGGCATGTAAGATCTCCAATGTGGAACTGACAAACCTGGTTTCATACATGGGTATTCTGCACGAAAAT
CGCTTGGGCCAGTCAGTACTCCATAGCACCCTGAAGGGGGAGGCACGGAGAGACTATATCCGCGATTGTG
TATATCTGTACCTCTGCCATACATGGCAGACTGGCATGGGTGTGTGGCAGCAATGTCTGGAAGAACAGAA
CCTCAAGGAACTTGACAAGCTGCTTCAGAAGAGCCTGAAGGTTCTCTGGACGGGATTTGATGAGCGAACT
GTGGCCTCTGACCTGGCTGATCTCATCTTCCCCGAAAGGCTCCGGGTGACCCTCCGCAATGGTCTGCCAG
ATTTTATGAGTCAGAGCATGCTGAATAACTTCCGGTCTTTTATCCTCGAACGCTCAGGTATACTGCCGGC
CACATGCTGCGCTTTGCCATCTGATTTTGTCCCCCTGACCTATCGGGAGTGCCCCCCCCCTCTGTGGTCC
CACTGTTATTTGTTCCGACTGGCTAACTACCTGTCCTATCACTCAGACGTAATTGAAGACGTGTCTGGCG
ATGGACTGCTGGAATGCCACTGTCGCTGTAATTTGTGCACTCCGCACCGGTCTCTCGCGTGCAACCCCCA
GCTCATGTCCGAGACACAGATTATTGGGACCTTCGAGCTCCAGGGGCCATCCTCCCAGGGGGACGGGTCC
AGCCCTGGGCAGTCCCTGAAGCTTACCCCCGGGCTGTGGACGTCTGCTTACTTGAGAAAATTTGCACCTG
AGGATTATCATCCATATGAGATAAAATTTTACGAAGATCAGTCACAGCCCCCGAAAGCAGAGTTGTCTGC
TTGCGTCATCACGCAAGGTGCCATCCTTGCTCAGCTCCAAGCGATTCAGAAGTCTCGCCAGGAGTTTCTG
TTGAAGAAGGGCAAAGGCGTCTACTTGGATCCACAGACTGGAGAGGAACTCAATACACGGTTTCCACAGG
ATGTGCCAACAACCCGGAAACAGGAGGTGGAGGGGGCTGCCGCCGCTCCCCGCAGGTATGGTGGCAGACT
TGGCCAAAGCGGGAGAGGAGGGGGAGGCGGGCAATCTGGAGGACAGTCTGGTGGACGACAATTTGGTGGA
GGCAAACGCGGCGGCCGGGGAGGTGGACGGTCTAATAGAAGACAGACGGTTATCTTGGGATGCGGAGATA
AACAACAGAGGTATCACTTGAGGTCCGAATCCAGAAACCCAGCGGCAAGCCAGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100090 representing AP_000588
Red=Cloning sites Green=Tags

METQPLLSTSPRVPSHLVLSDEEKEQSLSIPPPSPATTSTLEDKEVDASHDMQNKKAKESETDIEQDPGY
VTPVEHEEELKRFLEREDENCPKQRADNYHQDAGNRDQNTDYLIGLDGEDALLKHLARQSLIVKDALLDR
TEVPISVEELSCAYELNLFSPRTPPKRQPNGTCEPNPRLNFYPAFAVPEVLATYHIFFKNQKIPVSCRAN
RTRADALLNLGPGSRLPDIASLEEVPKIFEGLGNNETRAANALQKGENGMDEHHSVLVELEGDNARLAVL
KRSVEVTHFAYPAVNLPPKVMTAVMDQLLIKRASPLSEDMHDPDACDEGKPVVSDEQLTRWLGTDSPRDL
EERRKLMMAVVLVTVELECLRRFFTDSETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSYMGILHEN
RLGQSVLHSTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQKSLKVLWTGFDERT
VASDLADLIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRECPPPLWS
HCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLMSETQIIGTFELQGPSSQGDGS
SPGQSLKLTPGLWTSAYLRKFAPEDYHPYEIKFYEDQSQPPKAELSACVITQGAILAQLQAIQKSRQEFL
LKKGKGVYLDPQTGEELNTRFPQDVPTTRKQEVEGAAAAPRRYGGRLGQSGRGGGGGQSGGQSGGRQFGG
GKRGGRGGGRSNRRQTVILGCGDKQQRYHLRSESRNPAASQQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000019
ORF Size 2436 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000019.1, AP_000588
RefSeq ORF 2436 bp
MW 91.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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