BALF5 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101083

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BALF5 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129507



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CNY 15,390.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101083 represents NCBI reference of YP_001129507 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGGGGGCCTGTTCTATAACCCCTTTCTGCGGCCCAACAAGGGTCTGCTCAAGAAACCCGACAAGG
AATATCTGCGGTTGATTCCTAAGTGTTTTCAAACCCCCGGTGCAGCCGGGGTCGTGGACGTCAGGGGGCC
ACAACCGCCCCTGTGTTTTTACCAGGATTCATTGACCGTGGTGGGCGGAGACGAGGATGGCAAGGGTATG
TGGTGGAGGCAGCGCGCACAGGAAGGCACAGCGAGACCCGAAGCCGATACCCATGGGTCACCACTCGACT
TCCACGTGTATGATATCCTTGAGACCGTTTATACCCACGAAAAATGTGCGGTGATTCCTAGCGACAAACA
AGGCTACGTCGTCCCATGTGGCATCGTAATTAAGCTCCTCGGTAGACGGAAAGCCGACGGCGCTAGCGTT
TGCGTAAACGTCTTCGGTCAGCAGGCTTATTTCTACGCCAGCGCCCCGCAGGGTCTGGACGTTGAATTCG
CTGTGCTCAGCGCACTCAAGGCCAGTACCTTCGACAGACGCACACCATGTCGGGTGAGCGTTGAGAAGGT
GACAAGGAGAAGTATCATGGGGTACGGGAACCATGCCGGGGATTACCATAAGATTACCCTCAGCCACCCA
AACAGTGTCTGCCATGTGGCTACCTGGCTCCAGGACAAGCACGGCTGCCGCATCTTTGAGGCTAACGTCG
ACGCCACGAGGAGGTTTGTACTCGACAACGATTTTGTCACTTTCGGATGGTATAGCTGCAGGAGAGCTAT
TCCACGGCTCCAACATAGAGATAGCTACGCAGAACTGGAATACGACTGTGAGGTGGGCGATCTGAGTGTG
CGGAGAGAAGATTCCTCTTGGCCATCCTACCAAGCACTGGCGTTCGATATTGAGTGCCTCGGAGAAGAAG
GTTTTCCTACTGCGACGAACGAGGCAGACCTGATCTTGCAGATTTCATGCGTGCTGTGGTCAACAGGAGA
AGAGGCCGGGCGGTACAGACGAATCCTCCTGACCCTGGGAACCTGCGAAGACATCGAGGGCGTAGAGGTG
TACGAATTTCCCAGCGAGCTTGACATGCTCTATGCCTTCTTTCAGCTTATTCGCGACCTCTCTGTGGAGA
TCGTGACCGGCTATAATGTTGCCAATTTCGACTGGCCCTACATCCTGGACCGCGCCCGACACATTTACTC
CATTAATCCAGCTAGCCTGGGGAAAATAAGAGCTGGTGGCGTCTGTGAGGTGAGGAGGCCCCACGACGCT
GGAAAAGGATTCCTTAGAGCCAATACCAAGGTGCGGATAACTGGTCTGATCCCAATTGACATGTATGCTG
TGTGTAGGGACAAACTGTCTCTGAGTGACTATAAACTCGACACCGTCGCCAGGCATCTGCTGGGAGCTAA
AAAAGAGGATGTTCATTATAAGGAGATTCCCAGACTTTTCGCTGCTGGCCCCGAGGGCCGCAGGCGCCTT
GGGATGTACTGCGTCCAGGATTCCGCCCTGGTCATGGATCTGCTTAATCATTTCGTGATTCACGTGGAGG
TGGCCGAAATCGCCAAAATTGCTCATATCCCTTGCAGGCGAGTGTTGGACGATGGGCAGCAGATCCGAGT
GTTCTCATGCCTGCTGGCCGCAGCGCAGAAGGAGAATTTCATCCTGCCAATGCCCAGTGCCAGCGACAGA
GATGGTTACCAGGGGGCCACAGTGATTCAGCCACTGAGCGGATTTTACAATTCTCCGGTCCTGGTAGTCG
ACTTCGCTAGTCTCTATCCCTCAATCATTCAGGCACACAACCTCTGTTATTCAACCATGATCACACCCGG
CGAGGAACATAGGCTCGCTGGGCTTCGGCCCGGGGAGGACTACGAATCTTTCAGACTGACCGGTGGTGTA
TACCATTTTGTCAAGAAGCACGTTCATGAGTCCTTCCTCGCCTCCTTGTTGACTTCATGGCTGGCAAAGA
GAAAGGCGATAAAAAAACTCCTCGCGGCATGTGAGGATCCCAGGCAGCGGACTATTCTCGACAAGCAGCA
GTTGGCGATCAAGTGCACCTGTAACGCCGTATATGGCTTCACTGGCGTGGCTAACGGGCTGTTTCCCTGC
CTCAGCATTGCGGAGACGGTAACCCTCCAGGGAAGAACCATGCTGGAACGAGCCAAAGCATTTGTGGAGG
CCCTGTCTCCGGCCAACCTGCAAGCTTTGGCTCCTTCACCTGATGCCTGGGCACCACTTAATCCTGAGGG
TCAATTGAGGGTTATTTACGGGGATACAGACTCCTTGTTTATAGAATGTAGGGGATTTTCTGAATCTGAA
ACACTGCGGTTTGCTGAGGCCTTGGCCGCCCACACAACAAGGTCCCTTTTCGTTGCTCCAATCAGTTTGG
AAGCTGAAAAAACTTTCTCTTGTCTGATGCTGATCACTAAGAAACGGTACGTGGGCGTGCTGACCGACGG
CAAAACTCTGATGAAAGGGGTGGAGTTGGTCAGGAAGACAGCTTGTAAATTCGTGCAGACTCGCTGTCGC
AGAGTTCTGGATTTGGTTCTCGCAGACGCCAGGGTCAAGGAGGCTGCCTCCTTGTTGTCTCATAGGCCCT
TCCAGGAGTCATTCACACAGGGCCTTCCTGTAGGTTTCCTGCCAGTGATTGATATCCTCAATCAGGCGTA
CACAGACTTGCGGGAAGGGAGAGTGCCGATGGGCGAGCTTTGCTTTAGCACCGAGCTGAGTAGAAAGCTG
TCAGCTTATAAATCAACACAGATGCCCCATCTGGCCGTGTACCAGAAATTCGTTGAACGGAATGAAGAAC
TGCCCCAGATTCACGACAGAATCCAATATGTTTTCGTGGAACCCAAGGGCGGCGTGAAGGGAGCCAGAAA
GACAGAGATGGCTGAAGATCCAGCCTATGCAGAACGGCACGGAGTCCCAGTGGCTGTTGATCACTACTTC
GACAAACTGTTGCAGGGGGCTGCCAATATCCTGCAATGTCTGTTCGACAACAACAGCGGCGCTGCACTTA
GCGTGCTGCAGAACTTCACTGCCAGGCCCCCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101083 representing YP_001129507
Red=Cloning sites Green=Tags

MSGGLFYNPFLRPNKGLLKKPDKEYLRLIPKCFQTPGAAGVVDVRGPQPPLCFYQDSLTVVGGDEDGKGM
WWRQRAQEGTARPEADTHGSPLDFHVYDILETVYTHEKCAVIPSDKQGYVVPCGIVIKLLGRRKADGASV
CVNVFGQQAYFYASAPQGLDVEFAVLSALKASTFDRRTPCRVSVEKVTRRSIMGYGNHAGDYHKITLSHP
NSVCHVATWLQDKHGCRIFEANVDATRRFVLDNDFVTFGWYSCRRAIPRLQHRDSYAELEYDCEVGDLSV
RREDSSWPSYQALAFDIECLGEEGFPTATNEADLILQISCVLWSTGEEAGRYRRILLTLGTCEDIEGVEV
YEFPSELDMLYAFFQLIRDLSVEIVTGYNVANFDWPYILDRARHIYSINPASLGKIRAGGVCEVRRPHDA
GKGFLRANTKVRITGLIPIDMYAVCRDKLSLSDYKLDTVARHLLGAKKEDVHYKEIPRLFAAGPEGRRRL
GMYCVQDSALVMDLLNHFVIHVEVAEIAKIAHIPCRRVLDDGQQIRVFSCLLAAAQKENFILPMPSASDR
DGYQGATVIQPLSGFYNSPVLVVDFASLYPSIIQAHNLCYSTMITPGEEHRLAGLRPGEDYESFRLTGGV
YHFVKKHVHESFLASLLTSWLAKRKAIKKLLAACEDPRQRTILDKQQLAIKCTCNAVYGFTGVANGLFPC
LSIAETVTLQGRTMLERAKAFVEALSPANLQALAPSPDAWAPLNPEGQLRVIYGDTDSLFIECRGFSESE
TLRFAEALAAHTTRSLFVAPISLEAEKTFSCLMLITKKRYVGVLTDGKTLMKGVELVRKTACKFVQTRCR
RVLDLVLADARVKEAASLLSHRPFQESFTQGLPVGFLPVIDILNQAYTDLREGRVPMGELCFSTELSRKL
SAYKSTQMPHLAVYQKFVERNEELPQIHDRIQYVFVEPKGGVKGARKTEMAEDPAYAERHGVPVAVDHYF
DKLLQGAANILQCLFDNNSGAALSVLQNFTARPPF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 3045 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129507
RefSeq ORF 3045 bp
Locus ID 5176180
MW 113.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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