single-stranded DNA binding protein (NC_009334) Virus Tagged ORF Clone

CAT#: VC101086

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BALF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129510



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CNY 17,010.00


货期*
7周

规格
    • 10 ug

Cited in 13 publications.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101086 represents NCBI reference of YP_001129510 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGGGGCGCAAACTTCAGAGGACAATCTGGGCTCTCAGTCACAACCTGGACCCTGTGGATACATTT
ATTTCTATCCTCTGGCTACTTATCCACTCCGGGAAGTGGCTACCCTCGGGACCGGCTATGCAGGACATCG
ATGTCTGACTGTCCCACTGCTTTGCGGGATTACCGTCGAGCCCGGCTTTAGCATAAACGTGAAAGCCTTG
CATCGGCGACCTGATCCAAACTGCGGGCTCCTCCGAGCGACCTCTTACCACAGAGATATCTATGTGTTTC
ACAATGCCCACATGGTTCCTCCCATCTTTGAAGGGCCTGGTCTGGAGGCCCTGTGCGGAGAGACTCGCGA
GGTATTTGGTTACGATGCTTACTCCGCCCTCCCGCGAGAGAGCTCAGAACCTGGCGATTTTTTCCCTGAG
GGATTGGACCCATCCGCGTACTTGGGAGCCGTTGCTATCACGGAGGCCTTTAAGGAGAGACTGTATTCCG
GGAATCTTGTCGCCATCCCATCTCTGAAGCAAGAGGTGGCCGTGGGTCAGAGCGCATCCGTGCGGGTGCC
TCTTTACGATAAAGAAGTGTTCCCTGAAGGGGTGCCCCAGCTGAGACAGTTCTATAATTCCGATCTGTCT
CGCTGCATGCACGAAGCTTTGTACACTGGCCTCGCACAGGCACTCCGCGTACGGCGCGTGGGTAAACTGG
TCGAGCTGCTGGAAAAGCAAAGTCTCCAGGATCAGGCCAAAGTTGCCAAGGTGGCTCCACTGAAAGAGTT
CCCCGCAAGTACAATATCTCATCCGGATAGTGGCGCACTCATGATTGTCGATTCTGCCGCTTGCGAACTT
GCAGTCTCTTACGCCCCTGCCATGCTCGAAGCATCCCATGAGACTCCCGCCTCCCTTAATTACGACTCCT
GGCCATTGTTCGCTGATTGTGAGGGACCAGAAGCCCGCGTCGCAGCTCTCCACCGGTACAACGCTAGCCT
GGCCCCGCATGTGAGCACCCAGATTTTTGCTACCAACTCCGTGCTGTACGTCAGTGGGGTATCCAAGAGC
ACAGGGCAGGGAAAGGAGAGTCTGTTCAACAGCTTCTACATGACACACGGATTGGGCACTCTTCAAGAAG
GCACATGGGACCCCTGTAGAAGGCCCTGCTTTAGTGGATGGGGCGGACCTGATGTGACTGGGACTAATGG
CCCAGGGAACTACGCCGTGGAGCATCTGGTATATGCCGCTAGTTTCTCCCCCAACTTGCTGGCCCGGTAT
GCATACTACCTGCAGTTCTGTCAAGGACAAAAGAGTTCTTTGACCCCTGTACCCGAGACAGGCTCCTACG
TGGCGGGGGCTGCTGCTTCACCCATGTGTTCCTTGTGTGAGGGTCGAGCGCCTGCAGTTTGTCTGAATAC
CCTGTTTTTCCGCTTGCGCGATAGATTCCCGCCAGTCATGTCAACTCAACGGCGCGACCCCTATGTTATA
AGCGGGGCATCTGGGTCTTACAATGAAACCGACTTTCTGGGTAATTTTCTGAATTTTATCGATAAGGAAG
ATGATGGCCAGAGACCAGATGATGAACCACGGTACACCTACTGGCAACTGAACCAGAATCTGCTGGAAAG
GCTGAGTCGCCTTGGTATTGACGCTGAAGGGAAGCTCGAGAAAGAGCCACATGGACCTCGAGACTTCGTC
AAGATGTTCAAGGATGTAGACGCTGCAGTAGATGCCGAAGTGGTGCAATTTATGAACAGCATGGCAAAGA
ATAACATTACCTACAAAGATCTGGTGAAGAGCTGCTACCATGTTATGCAGTACTCATGCAACCCATTCGC
ACAACCTGCTTGTCCTATTTTTACTCAGCTCTTCTACCGCAGCCTTCTCACCATCCTGCAGGACATATCC
CTGCCTATCTGTATGTGCTACGAAAACGATAATCCAGGGCTGGGCCAGTCTCCGCCAGAGTGGTTGAAGG
GCCACTATCAGACCTTGTGTACTAATTTTAGAAGTCTCGCAATTGACAAGGGCGTTCTCACAGCAAAGGA
AGCAAAGGTGGTCCACGGCGAGCCAACATGTGACTTGCCTGACCTTGACGCCGCGCTGCAGGGACGAGTC
TATGGACGCAGACTGCCTGTTCGCATGAGTAAAGTTCTCATGCTGTGCCCACGGAATATTAAAATCAAAA
ACCGCGTTGTGTTTACCGGAGAAAACGCCGCTCTTCAGAATTCCTTCATCAAGTCCACAACCCGGAGAGA
GAATTATATCATCAACGGCCCATATATGAAGTTTCTGAACACTTACCACAAAACGCTCTTCCCTGACACT
AAGCTCTCAAGCCTCTACCTTTGGCACAATTTTTCACGGAGAAGAAGCGTCCCCGTGCCATCAGGAGCAA
GTGCCGAAGAGTATAGCGACTTGGCTCTCTTTGTAGATGGCGGGTCAAGGGCTCATGAGGAGAGCAATGT
TATCGATGTCGTCCCTGGGAACTTGGTAACATACGCTAAGCAGCGCTTGAACAACGCAATTCTTAAAGCA
TGCGGGCAGACCCAATTCTACATCTCCCTCATCCAGGGTTTGGTTCCCAGAACACAATCCGTGCCCGCCC
GCGACTACCCCCACGTTCTCGGAACCCGGGCTGTCGAGTCCGCTGCTGCCTACGCTGAAGCCACCTCAAG
CTTGACAGCCACCACGGTAGTGTGTGCAGCTACGGACTGTCTTTCACAGGTCTGTAAAGCGCGGCCTGTG
GTCACCCTGCCGGTGACGATAAATAAGTACACCGGCGTGAACGGCAATAACCAAATTTTCCAGGCCGGTA
ATCTCGGATATTTCATGGGAAGAGGAGTTGACAGGAATCTGCTGCAGGCCCCCGGCGCAGGGCTGCGCAA
ACAGGCAGGGGGTTCCTCAATGAGGAAAAAGTTCGTATTCGCTACCCCTACATTGGGTTTGACTGTGAAG
CGACGGACTCAAGCCGCCACCACCTACGAAATTGAAAATATCCGGGCTGGACTTGAGGCTATCATCAGTC
AAAAACAGGAGGAGGACTGTGTTTTCGACGTAGTTTGTAATCTCGTTGACGCGATGGGGGAAGCCTGCGC
CTCCCTTACGCGGGACGATGCCGAATATCTGCTGGGTCGCTTTTCTGTTCTCGCAGATAGTGTGCTTGAA
ACACTCGCTACGATCGCATCAAGCGGGATCGAATGGACCGCCGGCGCTGCCCGAGACTTTCTCGAGGGGG
TGTGGGGGGGTCCAGGCGCAGCGCAAGATAATTTCATTTCTGTGGCTGAACCTGTTTCAACCGCAAGCCA
GGCTAGTGCAGGCCTCCTGCTGGGAGGAGGCGGGCAGGGGAGCGGAGGCAGGAGAAAACGACGGCTGGCC
ACAGTCCTGCCTGGCTTGGAAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101086 representing YP_001129510
Red=Cloning sites Green=Tags

MQGAQTSEDNLGSQSQPGPCGYIYFYPLATYPLREVATLGTGYAGHRCLTVPLLCGITVEPGFSINVKAL
HRRPDPNCGLLRATSYHRDIYVFHNAHMVPPIFEGPGLEALCGETREVFGYDAYSALPRESSEPGDFFPE
GLDPSAYLGAVAITEAFKERLYSGNLVAIPSLKQEVAVGQSASVRVPLYDKEVFPEGVPQLRQFYNSDLS
RCMHEALYTGLAQALRVRRVGKLVELLEKQSLQDQAKVAKVAPLKEFPASTISHPDSGALMIVDSAACEL
AVSYAPAMLEASHETPASLNYDSWPLFADCEGPEARVAALHRYNASLAPHVSTQIFATNSVLYVSGVSKS
TGQGKESLFNSFYMTHGLGTLQEGTWDPCRRPCFSGWGGPDVTGTNGPGNYAVEHLVYAASFSPNLLARY
AYYLQFCQGQKSSLTPVPETGSYVAGAAASPMCSLCEGRAPAVCLNTLFFRLRDRFPPVMSTQRRDPYVI
SGASGSYNETDFLGNFLNFIDKEDDGQRPDDEPRYTYWQLNQNLLERLSRLGIDAEGKLEKEPHGPRDFV
KMFKDVDAAVDAEVVQFMNSMAKNNITYKDLVKSCYHVMQYSCNPFAQPACPIFTQLFYRSLLTILQDIS
LPICMCYENDNPGLGQSPPEWLKGHYQTLCTNFRSLAIDKGVLTAKEAKVVHGEPTCDLPDLDAALQGRV
YGRRLPVRMSKVLMLCPRNIKIKNRVVFTGENAALQNSFIKSTTRRENYIINGPYMKFLNTYHKTLFPDT
KLSSLYLWHNFSRRRSVPVPSGASAEEYSDLALFVDGGSRAHEESNVIDVVPGNLVTYAKQRLNNAILKA
CGQTQFYISLIQGLVPRTQSVPARDYPHVLGTRAVESAAAYAEATSSLTATTVVCAATDCLSQVCKARPV
VTLPVTINKYTGVNGNNQIFQAGNLGYFMGRGVDRNLLQAPGAGLRKQAGGSSMRKKFVFATPTLGLTVK
RRTQAATTYEIENIRAGLEAIISQKQEEDCVFDVVCNLVDAMGEACASLTRDDAEYLLGRFSVLADSVLE
TLATIASSGIEWTAGAARDFLEGVWGGPGAAQDNFISVAEPVSTASQASAGLLLGGGGQGSGGRRKRRLA
TVLPGLEV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 3384 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129510
RefSeq ORF 3384 bp
Locus ID 5176206
MW 123.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (13)

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