glycoprotein BALF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101084

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BALF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129508



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CNY 13,020.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101084 represents NCBI reference of YP_001129508 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACGGCGCCGGGTGTTGTCTGTCGTAGTCCTGCTTGCCGCTCTGGCCTGTCGCCTCGGCGCCCAGA
CACCTGAACAACCAGCACCACCGGCCACCACAGTCCAGCCCACAGCCACCAGACAACAGACGTCCTTCCC
ATTCAGAGTGTGTGAACTTTCCAGTCACGGCGACCTGTTTAGGTTTAGCAGCGATATCCAGTGCCCCTCT
TTCGGAACCAGGGAAAACCACACCGAGGGGCTCCTGATGGTGTTTAAGGACAATATTATCCCATATAGCT
TCAAAGTTCGGTCATACACAAAGATTGTGACCAACATCCTGATCTACAATGGCTGGTATGCAGATTCCGT
AACCAACAGACATGAGGAGAAATTCTCCGTGGATAGTTATGAGACCGACCAGATGGATACCATTTACCAG
TGCTACAACGCAGTTAAGATGACAAAGGATGGCCTTACCCGCGTGTACGTGGATCGAGACGGGGTGAATA
TAACAGTAAATCTCAAACCAACTGGGGGTCTGGCAAATGGCGTGAGGCGCTACGCCTCTCAGACTGAATT
GTACGATGCTCCCGGCTGGCTTATCTGGACCTACAGAACCCGGACCACAGTAAACTGCTTGATTACTGAC
ATGATGGCGAAGAGTAACAGTCCATTTGACTTTTTTGTAACGACTACAGGCCAGACGGTGGAGATGTCTC
CTTTCTATGATGGTAAAAATAAGGAGACGTTTCATGAGCGCGCAGATTCATTTCACGTCCGGACCAACTA
TAAAATAGTGGACTACGATAACAGGGGGACCAATCCCCAGGGGGAGCGACGCGCCTTTCTGGATAAAGGC
ACATATACTCTCTCATGGAAACTGGAAAACCGGACTGCATATTGTCCTCTGCAGCACTGGCAGACTTTTG
ATAGTACAATCGCCACCGAAACGGGGAAATCCATCCACTTTGTGACGGATGAAGGCACTTCTTCCTTCGT
GACTAACACCACAGTCGGCATTGAGCTGCCCGATGCATTCAAGTGTATCGAAGAGCAGGTGAACAAAACG
ATGCATGAGAAGTACGAAGCAGTGCAGGACAGATACACCAAGGGCCAGGAGGCCATTACGTACTTCATCA
CTTCCGGCGGTCTGCTCCTTGCCTGGCTGCCGCTCACTCCTCGAAGTCTGGCCACCGTCAAGAACCTCAC
TGAGCTGACAACCCCCACATCTTCCCCTCCGTCTTCTCCATCCCCACCCGCCCCTCCAGCGGCGAGAGGA
TCTACCAGCGCAGCCGTGCTGAGAAGACGAAGACGCGACGCAGGCAACGCGACAACTCCTGTTCCTCCCG
CCGCACCCGGTAAATCTCTGGGAACCCTGAACAACCCAGCTACTGTGCAAATTCAGTTCGCATATGATAG
TCTGAGAAGGCAGATCAATAGAATGCTGGGCGATCTCGCCCGAGCCTGGTGCTTGGAGCAGAAGCGGCAA
AATATGGTCTTGAGAGAGCTGACAAAGATCAACCCCACAACTGTTATGTCAAGCATTTACGGTAAGGCTG
TTGCAGCTAAGCGACTCGGTGATGTGATTAGCGTGAGTCAGTGTGTGCCAGTCAATCAGGCAACTGTGAC
ACTTCGCAAGTCAATGCGGGTCCCCGGGAGTGAGACAATGTGTTATTCTCGGCCTCTGGTTTCATTCAGT
TTTATCAACGACACTAAGACCTATGAAGGGCAACTTGGAACAGACAATGAGATTTTCCTGACCAAGAAGA
TGACTGAGGTGTGTCAGGCCACTAGCCAGTACTATTTCCAGAGCGGGAACGAAATTCACGTGTATAATGA
TTACCACCACTTTAAGACCATTGAACTGGACGGCATAGCCACACTCCAGACTTTCATTTCCCTGAATACC
AGTCTGATAGAAAATATTGACTTTGCCAGCCTGGAACTGTACAGCCGCGACGAGCAGAGAGCCTCCAATG
TGTTTGACCTGGAAGGGATTTTTCGAGAGTACAATTTCCAGGCACAGAACATAGCTGGGCTGAGAAAGGA
CCTGGACAATGCTGTTAGCAATGGTCGGAATCAATTTGTGGACGGCCTGGGGGAACTGATGGATAGTCTG
GGCAGCGTCGGTCAGAGCATAACCAACCTGGTGTCCACTGTTGGGGGACTCTTTTCCTCTCTCGTGAGTG
GCTTTATTTCATTTTTTAAGAACCCATTTGGGGGAATGCTGATCCTGGTCTTGGTGGCAGGCGTCGTGAT
CCTGGTAATCAGCCTCACCAGGAGAACTAGACAGATGTCCCAGCAGCCGGTACAGATGTTGTATCCCGGC
ATCGACGAGTTGGCTCAACAGCACGCCAGCGGGGAAGGTCCAGGAATCAATCCTATAAGCAAAACCGAGC
TCCAGGCCATTATGCTGGCCCTGCACGAACAAAATCAAGAACAGAAAAGAGCGGCCCAGCGCGCAGCCGG
GCCAAGCGTCGCCAGTAGAGCCCTTCAGGCAGCTAGAGATCGGTTCCCCGGACTCCGAAGACGCAGGTAT
CATGACCCCGAAACAGCAGCCGCGCTTCTCGGTGAGGCCGAGACCGAATTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101084 representing YP_001129508
Red=Cloning sites Green=Tags

MTRRRVLSVVVLLAALACRLGAQTPEQPAPPATTVQPTATRQQTSFPFRVCELSSHGDLFRFSSDIQCPS
FGTRENHTEGLLMVFKDNIIPYSFKVRSYTKIVTNILIYNGWYADSVTNRHEEKFSVDSYETDQMDTIYQ
CYNAVKMTKDGLTRVYVDRDGVNITVNLKPTGGLANGVRRYASQTELYDAPGWLIWTYRTRTTVNCLITD
MMAKSNSPFDFFVTTTGQTVEMSPFYDGKNKETFHERADSFHVRTNYKIVDYDNRGTNPQGERRAFLDKG
TYTLSWKLENRTAYCPLQHWQTFDSTIATETGKSIHFVTDEGTSSFVTNTTVGIELPDAFKCIEEQVNKT
MHEKYEAVQDRYTKGQEAITYFITSGGLLLAWLPLTPRSLATVKNLTELTTPTSSPPSSPSPPAPPAARG
STSAAVLRRRRRDAGNATTPVPPAAPGKSLGTLNNPATVQIQFAYDSLRRQINRMLGDLARAWCLEQKRQ
NMVLRELTKINPTTVMSSIYGKAVAAKRLGDVISVSQCVPVNQATVTLRKSMRVPGSETMCYSRPLVSFS
FINDTKTYEGQLGTDNEIFLTKKMTEVCQATSQYYFQSGNEIHVYNDYHHFKTIELDGIATLQTFISLNT
SLIENIDFASLELYSRDEQRASNVFDLEGIFREYNFQAQNIAGLRKDLDNAVSNGRNQFVDGLGELMDSL
GSVGQSITNLVSTVGGLFSSLVSGFISFFKNPFGGMLILVLVAGVVILVISLTRRTRQMSQQPVQMLYPG
IDELAQQHASGEGPGINPISKTELQAIMLALHEQNQEQKRAAQRAAGPSVASRALQAARDRFPGLRRRRY
HDPETAAALLGEAETEF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 2571 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129508
RefSeq ORF 2571 bp
Locus ID 5176207
MW 95.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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