Neurofibromin (NF1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	Neurofibromin
Locus ID	4763
Kit Components	KN220378G1, Neurofibromin gRNA vector 1 in pCas-Guide CRISPR vector KN220378G2, Neurofibromin gRNA vector 2 in pCas-Guide CRISPR vector KN220378LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet CTCCCGGGTC AGCTCTGGCA CTCGCCAGCT GAGCCCAGCG CCAGTCTAGG TGAGCCCCAC GGCGGTGAGG GACGCTCGCC AGACGGCCCA GAGGAGTTAG ATGACGTCAC CTCCAGGAGG ACTCGCTTTT TCATTAATGA AACCGGCCGG CGCGGGCGCA TGCGCGGCAG GCCGCCTTCC CTCTCGCTTC CCCCTCCCCT TTCCCAGCCG CGCTCTCAAT CTCTAGCTCG CTCGCGCTCC CTCTCCCCGG GCCGTGGAAA GGATCCCACT TCCGGTGGGG TGTCATGGCG GCGTCTCGGA CTGTGATGGC TGTGGGGAGA CGGCGCTAGT GGGGAGAGCG ACCAAGAGGC CCCCTCCCCT CCCCGGGTCC CCTTCCCCTA TCCCCCTCCC CCCAGCCTCC TTGCCAACGC CCCCTTTCCC TCTCCCCCTC CCGCTCGGCG CTGACCCCCC ATCCCCACCC CCGTGGGAAC ACTGGGAGCC TGCACTCCAC AGACCCTCTC CTTGCCTCTT CCCTCACCTC AGCCTCCGCT CCCCGCCCTC TTCCCGGCCC AGGGCGCCGG CCCACCCTTC CCTCCGCCGC CCCCCGGCCG CGGGGAGGAC AGTAGGTGAG GGGAGGTAGG AGCGGCCGCC TCCCCCGCGG CTGCCTCAGG CTCTGGAGGA AAGGAAGGGA AGGGAAGAGA AGGGAAGGGG GGATAAGTGG GGGTGGCCAA GGCGGGAGGT GAGGGGTAGG AGGGGACAGC TGGGAGCTTG GGCACCCTTT CCCTCCTAAG TCGGGGGGTG GGGCCTTGTC CCTGACCAGC CTCCGACCCT CCATCCCCTT TATCCCAGCC CTTCCGCTTG GAAATGGGGA TGAGTGACCT GGGGGCGCCT TTAGGGGCGC GCCATCTGGA TTTAATAATT ACCCCCTTAC TTTTGTTTTT GAGCAGAGGT GGGTAACGTT TAGGGGTTCT CTCTTCCTCT TCCCTCCGTG ACCCTCCTAA CTGGGGACGA GCGAACCCTA AGGCCTGTCT TATATACCCT CAATAGGAGA ACGATAACAT TCCGCCCCTC CCCCAGTTTC TTCAGCTTCC TCTCCTGGAG GGGGAAGAGC AGAAGAAAGC AAGGAAGGCA CAGGTTTTCT TACTTTCTGC TCCATCGTCT CATCCTGCCC CGAGAGCTTC AAGTGCACAC TCACTGCACA TCTTTGTGAT GCCCTACAAC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000267, NM_001042492, NM_001128147
Synonyms	NFNS; VRNF; WSS
Summary	This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008]

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