Selenop Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Selenop
Locus ID	20363
Kit Components	KN315523G1, Selenop gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTTGTTACAAAGCCCCGGAG KN315523G2, Selenop gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTCTGCTCCTCCATAGGGG KN315523-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CTGGCACTTT ACAGGAAGAT TTCATGCAGG AAGAGCTAAG GGCCACCATG AACCACAGCC ACCATGTTGG TCTCCACCGC CATGTCCTCC CTTTACTTTC TGGGCTGATT TTTTTTTTTT TTCAGTTTTG TGCAGAGTTC ACAACTAACA TTTCCTAGAT TGGCAGAGGA TAGAATGAAG CCATCAGGGC TCAGTGCAGC TCAAATGTGT CAGAATGTGT TTGCAGAATG TTCTGTGACT ATGCATTACA GTTGGAAGGT GTTTCTGAAT ATGTGTCCTT TCCCAGAGAG TTGCCTGGCT GTCTCATTAG GCACATTTCC AAATGTGTGC TGCTCATGGA AATACTGTAC CATGCTGCGG GTAGCTAGCT ACCTCTGATC ATTCCTCTGC CTTTTTGCTG GCACTTTCCC CTTTACAGGA ATATTTAATG GAAGGAGGCT CATGACAATG GCGTAATGAG CCTAACAAAA TGGAAATAGA ATCCATATGC TAAGTTATAA TAAGTAGCAT TGTACCTACC GTGTTTAAAG AAATTCATAG CAATGACTGT GAAGTTATCA ATGAAGATTA CATCTCTTGT TTTATAGGAC AACCTCAGCA AGTGACAGTG GTTGCTCTTC TTCAAGCCAG CTGATACTTG TGTCTTCTGC AGGCATCCAG GTAAGTGATC TTTTAAGGCT TAGGATATTC TTAGCGAAAG GTTATTAAAT ATTAATGTAT GCATATAAAA GGCAATATAA ATAGGAATAA TGTAATGGTT AAATAACACC TTTGAAGACA AATATGTTTG CATAGCACAC ATCTAATGAA AAGATGAGGG CATCTTATAG CTGTACACAC ACACACACAC ACACACACAC ATACACACAC ACAAACACAC ACATACACAC CATACACCAT GGATATATCC AGTACTACCA AAATGTTTAC TGTGGCATTC CTGGGCTTTG AACATGTTTT CCTATTAGTG GAGCAGATTC TTTAAGAGCA GAGAAAGGCT GCAAGCCAGT ACACACACTA AATGCTAAGA TGCTAATAAA AGGTCCTGCA TCATTTAACA ACCATAAACA AAAAACATTG GTTGACTCAT CCACCAACTA TTAGTGGCAT AACATTCTGT ATATATGCAA AGTGAATCTA AGTTTATCTA ATTTGAAGCT CATGTTCATG CAAACATTTA GCATTGTTTC AAGTTATAAA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001042613, NM_001042614, NM_009155
Synonyms	AU018766; D15Ucla1; Se-P; selp; Sepp1
Summary	This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in mouse), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. Alternatively spliced transcript variants differing in 5' non-coding region have been described for this gene. Expression of these variants varies in different tissues and developmental stages (PMID:23064117). [provided by RefSeq, Feb 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.