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p53 (TP53) (NM_000546) Human Over-expression Lysate
CAT＃: LC400186
TP53 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "p53" proteins (16)
|
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CNY 900.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC200003 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 43.7 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_000537 |
| Locus ID | 7157 |
| Cytogenetics | 17p13.1 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway |
Citations (4)
| The use of this Proteins has been cited in the following citations: |
|---|
|
O-GlcNAcylated p53 in the liver modulates hepatic glucose production
,null,
Nature Communications
,PubMed ID 34417460
[TP53]
|
|
AXL Inactivation Inhibits Mesothelioma Growth and Migration via Regulation of p53 Expression
,null,
Cancers
,PubMed ID 32992696
[TP53]
|
|
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition.
,null,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
,PubMed ID 30514107
[TP53]
|
|
Interaction of Mutant Hepatitis B X Protein With p53 Tumor Suppressor Protein Affects Both Transcription and Cell Survival
,null,
Molecular carcinogenesis
,PubMed ID 21438026
[TP53]
|
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Other Versions
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|---|---|---|---|
| LC426652 | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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| LC426653 | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
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| LC426654 | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
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| LY426653 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 4 |
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