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p53 (TP53) (NM_001126112) Human Over-expression Lysate
CAT＃: LC426652
TP53 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "p53" proteins (16)
|
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CNY 900.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC225604 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 43.5 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_001119584 |
| Locus ID | 7157 |
| Cytogenetics | 17p13.1 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway |
Citations (3)
| The use of this Proteins has been cited in the following citations: |
|---|
|
Akt inhibitor augments anti-proliferative efficacy of a dual mTORC1/2 inhibitor by FOXO3a activation in p53 mutated hepatocarcinoma cells
,null,
Cell Death & Disease
,PubMed ID 34759291
[TP53]
|
|
A combination of AZD5363 and FH5363 induces lethal autophagy in transformed hepatocytes
,null,
Cell Death & Disease
,PubMed ID 32681102
[TP53]
|
|
Inhibition of DYRK1A-EGFR axis by p53-MDM2 cascade mediates the induction of cellular senescence
,null,
Cell Death & Disease
,PubMed ID 30910997
[TP53]
|
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC400186 | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC426653 | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC426654 | TP53 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY400186 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 1 |
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| LY426652 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 2 |
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| LY426653 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 4 |
CNY 3,080.00 |
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| LY426654 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 3 |
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| PH300003 | TP53 MS Standard C13 and N15-labeled recombinant protein (NP_000537) |
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| PH325604 | TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119584) |
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| TP300003 | Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, 20 µg |
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| TP325502 | Purified recombinant protein of Homo sapiens tumor protein p53 (TP53), transcript variant 3, 20 µg |
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| TP325604 | Recombinant protein of human tumor protein p53 (TP53), transcript variant 2, 20 µg |
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| TP701001 | Purified recombinant protein of human tumor protein P53 (TP53), transcript variant 1, mutant (Y220C), expressed in HEK293 cells, 20ug |
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| TP710022 | Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9 cells. |
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| TP750119 | Purified recombinant protein of Human tumor protein p53 (TP53), transcript variant 1, full length, N-terminal His and GST tag, expressed in E. coli, 50ug |
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