WNT5A (NM_003392) Human Over-expression Lysate

Specifications

Product Data
Description	WNT5A HEK293T cell transient overexpression lysate (as WB positive control)
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	TrueORF Clone RC209206
Tag	C-Myc/DDK
Detection Antibodies	Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
Predicted MW	42.3 kDa
Components	1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage	The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Preparation	HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
RefSeq	NP_003383
Locus ID	7474
Cytogenetics	3p14.3
Protein Families	WB
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.