Junctional Adhesion Molecule 1 (F11R) (NM_144504) Human Over-expression Lysate
CAT#: LC403392
F11R HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "Junctional Adhesion Molecule 1" proteins (9)
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CNY 900.00
Cited in 1 publication. |
CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | F11R HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC200004 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 32.6 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_653087 |
Locus ID | 50848 |
Cytogenetics | 1q23.3 |
Protein Families | WB |
Protein Pathways | Cell adhesion molecules (CAMs), Epithelial cell signaling in Helicobacter pylori infection, Leukocyte transendothelial migration, Tight junction |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Junctional adhesion molecule-A is overexpressed in advanced multiple myeloma and determines response to oncolytic reovirus
,null,
Oncotarget
,PubMed ID 26513296
[F11R]
|
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LC413796 | F11R HEK293T cell transient overexpression lysate (as WB positive control) |
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TP300004 | Recombinant protein of human F11 receptor (F11R), transcript variant 5, 20 µg |
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