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Junctional Adhesion Molecule 1 (F11R) (NM_016946) Human Over-expression Lysate
CAT＃: LC429529
F11R HEK293T cell transient overexpression lysate (as WB positive control)
View other "Junctional Adhesion Molecule 1" proteins (9)
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CNY 900.00
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CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | F11R HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC221478 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 32.58 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_058642 |
| Locus ID | 50848 |
| Cytogenetics | 1q23.3 |
| Protein Families | WB |
| Protein Pathways | Cell adhesion molecules (CAMs), Epithelial cell signaling in Helicobacter pylori infection, Leukocyte transendothelial migration, Tight junction |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC403392 | F11R HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC413796 | F11R HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY403392 | Transient overexpression lysate of F11 receptor (F11R), transcript variant 5 |
CNY 3,080.00 |
|
| LY413796 | Transient overexpression lysate of F11 receptor (F11R) |
CNY 3,080.00 |
|
| PH300004 | F11R MS Standard C13 and N15-labeled recombinant protein (NP_653087) |
CNY 19,520.00 |
|
| PH321478 | F11R MS Standard C13 and N15-labeled recombinant protein (NP_058642) |
CNY 19,520.00 |
|
| TP300004 | Recombinant protein of human F11 receptor (F11R), transcript variant 5, 20 µg |
CNY 2,900.00 |
|
| TP321478 | Recombinant protein of human F11 receptor (F11R), 20 µg |
CNY 2,900.00 |
|
| TP720393 | Recombinant protein of human F11 receptor (F11R) |
CNY 3,140.00 |
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