PI 3 Kinase p85 alpha (PIK3R1) (NM_181504) Human Over-expression Lysate
CAT#: LC403621
PIK3R1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "PI 3 Kinase p85 alpha" proteins (5)
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CNY 900.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | PIK3R1 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC214266 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 53.3 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_852556 |
Locus ID | 5295 |
Cytogenetics | 5q13.1 |
Protein Families | Druggable Genome |
Protein Pathways | Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway |
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Other Versions
SKU | Description | Size | Price |
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LY403621 | Transient overexpression lysate of phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 2 |
CNY 3,080.00 |
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PH314266 | PIK3R1 MS Standard C13 and N15-labeled recombinant protein (NP_852556) |
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TP314266 | Recombinant protein of human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 2, 20 µg |
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TP710387 | Purified recombinant protein of Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9 cells, 20ug |
CNY 4,020.00 |
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TP762207 | Purified recombinant protein of Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, Ala278-Ser660, with N-terminal His tag, expressed in E.coli, 50ug |
CNY 1,948.00 |
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