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KRAS (NM_033360) Human Over-expression Lysate
CAT＃: LC409579
KRAS HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "KRAS" proteins (8)
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CNY 900.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Description | KRAS HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC222697 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 21.5 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_203524 |
| Locus ID | 3845 |
| Cytogenetics | 12p12.1 |
| Protein Families | WB |
| Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
|
Grifolin, neogrifolin and confluentin from the terricolous polypore Albatrellus flettii suppress KRAS expression in human colon cancer cells
,null,
PLoS ONE
,PubMed ID 32369483
[KRAS]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC417614 | KRAS HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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| LY409579 | Transient overexpression lysate of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant a |
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| LY417614 | Transient overexpression lysate of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b |
CNY 3,080.00 |
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| PH301958 | KRAS MS Standard C13 and N15-labeled recombinant protein (NP_004976) |
CNY 19,520.00 |
|
| PH322697 | KRAS MS Standard C13 and N15-labeled recombinant protein (NP_203524) |
CNY 19,520.00 |
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| TP301958 | Recombinant protein of human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, 20 µg |
CNY 2,900.00 |
|
| TP322697 | Recombinant protein of human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant a, 20 µg |
CNY 2,900.00 |
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| TP700052 | KRAS mutant (G12D) Myc-DDK-tagged human recombinant protein of Homo sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b |
CNY 6,650.00 |
KRAS mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)
