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EGFR (NM_005228) Human Over-expression Lysate
CAT＃: LC417434
EGFR HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "EGFR" proteins (15)
|
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CNY 1,470.00
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CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | EGFR HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC217384 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 134.7 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_005219 |
| Locus ID | 1956 |
| Cytogenetics | 7p11.2 |
| Protein Families | WB |
| Protein Pathways | Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC404514 | EGFR HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LC404515 | EGFR HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY404514 | Transient overexpression lysate of epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), transcript variant 2 |
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| LY404515 | Transient overexpression lysate of epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), transcript variant 3 |
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|
| LY417434 | Transient overexpression lysate of epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), transcript variant 1 |
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| PH314877 | EGFR MS Standard C13 and N15-labeled recombinant protein (NP_958440) |
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| TP314877 | Recombinant protein of human epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), transcript variant 3, 20 µg |
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| TP700080 | Purified protein of Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1, mutant K745_K750del, with C-terminal MYC/DDK tag, expressed in human cells, 20ug |
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| TP700103 | Purified recombinant protein of Human epidermal growth factor receptor (EGFR), transcript variant 3, with C-terminal DDK/His tag, expressed in human cells |
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| TP700204 | Purified recombinant protein of Human epidermal growth factor receptor (EGFR) extracellular domain, residues Leu25-Gly378, transcript variant 3 |
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| TP710011 | Recombinant protein of human epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), full length, with C-terminal DDK tag, expressed in sf9 cells |
CNY 4,020.00 |
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| TP710358 | Purified recombinant protein of Human epidermal growth factor receptor (EGFR), transcript variant 1, esidues 669-1210aa, with C-terminal DDK tag, expressed in sf9, 20ug |
CNY 4,020.00 |
