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DIAPH1 (NM_005219) Human Over-expression Lysate
CAT＃: LC417437
DIAPH1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "DIAPH1" proteins (8)
|
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CNY 1,470.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | DIAPH1 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC216318 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 141.2 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_005210 |
| Locus ID | 1729 |
| Cytogenetics | 5q31.3 |
| Protein Families | WB |
| Protein Pathways | Focal adhesion, Regulation of actin cytoskeleton |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
|
EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis
,null,
The Journal of Experimental Medicine
,PubMed ID 28827448
[DIAPH1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC421547 | DIAPH1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LC429246 | DIAPH1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LY417437 | Transient overexpression lysate of diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 1 |
CNY 4,840.00 |
|
| LY421547 | Transient overexpression lysate of diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2 |
CNY 4,840.00 |
|
| PH313517 | DIAPH1 MS Standard C13 and N15-labeled recombinant protein (NP_001073280) |
CNY 19,520.00 |
|
| PH316318 | DIAPH1 MS Standard C13 and N15-labeled recombinant protein (NP_005210) |
CNY 14,250.00 |
|
| TP313517 | Recombinant protein of human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2, 20 µg |
CNY 2,900.00 |
|
| TP316318 | Recombinant protein of human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 1, 20 µg |
CNY 2,900.00 |
