Toxoplasma gondii p30 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Concentration	lot specific
Purity	>95% pure (10% PAGE, coomassie staining) - GS-4B Sepharose-Affinity purification
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 50 mM Tris-HCl, 1.5M urea containing 50% glycerol
Preparation	Liquid purified protein
Applications	ELISA. Western Blot.
Protein Description	Recombinant Protein Toxoplasma gondii P30 (SAG1). Contains the P30 (SAG1) immunodominant region. Contains GST fusion partner. Immunoreactive with Toxoplasma gondii positive sera.
Storage	Store the protein at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
Synonyms	Major surface antigen p30
Summary	Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
Protein Families	ELISA. Western Blot.

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