Cystatin-B Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
Predicted MW	13 kDa
Concentration	lot specific
Purity	>98% by SDS PAGE
Buffer	Presentation State: Purified State: Liquid purified protein by using conventional chromatography Buffer System: 20 mM Tris pH 8.0, 50 mM NaCl
Preparation	Liquid purified protein by using conventional chromatography
Protein Description	Recombinant Human CSTB, fused to His-tag, was expressed in E.coli.
Note	NCBI Accession No.: NP_000091
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Endotoxin	< 1.0 EU per 1 microgram of protein (determined by LAL method)
Reference Data
RefSeq	NP_000091
Locus ID	1476
UniProt ID	P04080, Q76LA1
Cytogenetics	21q22.3
Synonyms	CPI-B; CST6; EPM1; EPM1A; PME; STFB; ULD
Summary	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]

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