G-CSF Rat Protein

Specifications

Product Data
Species	Rat
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MKKIPLLTVS SLPPSLPLPR SFLLKSLEQV RKIQARNTEL LEQLCATYKL CHPEELVLFG HSLGIPKASL SSCSSQALQQ TKCLSQLHSG LFLYQGLLQA LAGISSELAP TLDMLHLDVD NFATTIWQQM ESLGVAPTVQ PTQSTMPIFT SAFQRRAGGV LVTSYLQSFL ETAHHALHHL PRPAQKHFPE SLFISI
Predicted MW	21.6 kDa
Purity	>98% pure by SDS-PAGE gel and HPLC analyses
Buffer	Presentation State: Purified State: Lyophilized (0.2µ Sterile filtered) purified protein Buffer System: 10 mM Sodium Citrate, pH 4.0
Preparation	Lyophilized (0.2µ Sterile filtered) purified protein
Protein Description	Recombinant Rat G-CSF is a 21.6 kDa protein consisting of 196 amino acid residues.
Note	Centrifuge the vial prior to opening.
Storage	Prior to reconstitution store at 2-8°C for 6 months, at RT for one month or at -20°C to -80°C for longer. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Determined by its ability to stimulate the proliferation of Mouse NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. Specific: ≥ 2 x 107 units/mg.
Endotoxin	< 0.1 ng/μg of protein (< 1EU/μg)
Reference Data
RefSeq	NP_058800
Locus ID	25610
Cytogenetics	10q31
Synonyms	CSF3, GCSF, Pluripoietin, C17orf33
Summary	putative hematopoetic growth factor for neutrophils; used as a treatment for cyclic hematopoesis in humans and dogs [RGD, Feb 2006]

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