CD274 / PDL1 (Fc portion) Human Protein

Specifications

Product Data
Species	Human
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Monomer: FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER GGPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK
Predicted MW	102.6 kDa
Purity	>95% pure by SDS-PAGE and HPLC analyses
Buffer	Presentation State: Purified State: Lyophilized (sterile filtered) purified protein Buffer System: 10 mM Sodium Phosphate + 25 mM NaCl, pH 7.6
Preparation	Lyophilized (sterile filtered) purified protein
Protein Description	CHO cell-derived Recombinant Human PD-L1 Fc is a glycosylated, disulfide-linked homodimer of 906 amino acid residues whose monomer consists of the 220-amino-acid length extracellular portion of PD-L1 fused to the 231-amino-acid length Fc portion of human IgG1 by two glycines. The calculated molecular weight of CHO cell-derived Recombinant Human PD-L1 Fc is 102.6 kDa, however, due to glycosylation, it migrates at an apparent molecular weight of approximately 160-170 kDa by SDS-PAGE analysis under non-reducing conditions.
Note	Centrifuge the vial prior to opening!
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Determined by its ability to induce adhesion in T-cell enriched PBMC cultures. The ED50 for this effect is 1.2-2.0 μg/ml.
Endotoxin	< 0.1 ng per µg (1EU/µg)
Reference Data
RefSeq	NP_001254635
Locus ID	29126
UniProt ID	Q9NZQ7
Cytogenetics	9p24.1
Synonyms	PD-L1, PDCD1 ligand 1, B7H1, B7H1, B7 homolog 1, PDCD1L1, PDCD1LG1
Summary	This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)

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