MRPS25 (1-173, His-tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MGSMPMKGRF PIRRTLQYLS QGNVVFKDSV KVMTVNYNTH GELGEGARKF VFFNIPQIQY KNPWVQIMMF KNMTPSPFLR FYLDSGEQVL VDVETKSNKE IMEHIRKILG KNEETLREEE EEKKQLSHPA NFGPRKYCLR ECICEVEGQV PCPSLVPLPK EMRGKYKAAL KADAQD
Tag	His-tag
Predicted MW	22.5 kDa
Concentration	lot specific
Purity	>90% by SDS - PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol, 0.4M Urea
Preparation	Liquid purified protein
Protein Description	Recombinant human MRPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli .
Storage	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_071942
Locus ID	64432
UniProt ID	P82663
Cytogenetics	3p25.1
Synonyms	COXPD50; MRP-S25; RPMS25
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

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