Midkine (His-tagged Fusion Protein) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D
Tag	His-tag
Concentration	lot specific
Purity	>95% pure by SDS-PAGE.
Buffer	Presentation State: Purified State: Lyophilized (0.4 µm filtered) protein. Buffer System: 0.05M Phosphate buffer, 0.1M NaCl, pH 7.2
Preparation	Lyophilized (0.4 µm filtered) protein.
Applications	ELISA. Western blotting.
Protein Description	The Human Midkine is created as a recombinant protein with N-terminal fusion of HisTag. The Human Midkine His-Tagged Fusion Protein, produced in E. coli, is 14.6 kDa protein containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues - HisTag (underlined). The amino acid sequence of the recombinant human Midkine is 100% homologous to the amino acid sequence of the human Midkine without signal sequence.
Note	Purification: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Storage	Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 2-8°C for a limited period of time. It does not show any change after two weeks at 2-8°C.
Stability	Shelf life: One year from despatch.
Reference Data
RefSeq	NP_001012333
Locus ID	4192
UniProt ID	P21741
Cytogenetics	11p11.2
Synonyms	MK, MDK, MK1, NEGF2, Neurite outgrowth-promoting protein
Summary	This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]
Protein Families	ELISA. Western blotting.

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