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GBA (NM_000157) Human Recombinant Protein
CAT＃: TP316061
Recombinant protein of human glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 1, 20 µg
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Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>RC216061 representing NM_000157
Red=Cloning site Green=Tags(s) MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTF PALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQ NLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRP VSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLL SGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVH WYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDW NLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMH PDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 55.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Bioactivity | The enzymatic activity of TP316061 (GBA) was measured by its ability to hydrolyze a fluorescent substrate 4-methylumbelliferyl-β-D-glucopyranoside. The specific activity is > 70,000 pmol/hour/µg, as measured under the following conditions: 27 ng of GBA was incubated with 10 mM 4-methylumbelliferyl- β-D-glucopyranoside in the following buffer at 37°C for 40 min: 150 mM citrate-phosphate buffer, pH 5.4, 0.25% (w/w) sodium taurocholate, 0.25% (w/w) Triton X-100, and 1% bovine serum albumin. The reaction was terminated by adding 0.5 volume of 1M glycine buffer, pH 12.5. The hydrolyzed product of reaction, 4-methylumbelliferone (4-MU), was measured using a FlexStation 3 microplate reader (Ex365/Em445). Specific activity of GBA was calculated based on a standard curve of known concentration of 4-MU. |
| Reference Data | |
| RefSeq | NP_000148 |
| Locus ID | 2629 |
| UniProt ID | P04062 |
| Refseq Size | 2324 |
| Cytogenetics | 1q22 |
| Refseq ORF | 1608 |
| Synonyms | GBA1; GCB; GLUC |
| Summary | This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010] |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC400055 | GBA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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| LC423641 | GBA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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| LC423642 | GBA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
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| LC425145 | GBA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC433165 | GBA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY400055 | Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 1 |
CNY 3,080.00 |
|
| LY423641 | Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 2 |
CNY 3,080.00 |
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| LY423642 | Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 3 |
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| LY425145 | Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 3 |
CNY 3,080.00 |
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| LY433165 | Transient overexpression lysate of glucosidase, beta, acid (GBA), transcript variant 5 |
CNY 3,080.00 |
|
| PH301614 | GBA MS Standard C13 and N15-labeled recombinant protein (NP_001005741) |
CNY 14,250.00 |
|
| PH312242 | GBA MS Standard C13 and N15-labeled recombinant protein (NP_001005742) |
CNY 14,250.00 |
|
| PH316061 | GBA MS Standard C13 and N15-labeled recombinant protein (NP_000148) |
CNY 19,520.00 |
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| TP301614 | Purified recombinant protein of Homo sapiens glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 2, 20 µg |
CNY 2,900.00 |
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| TP312242 | Purified recombinant protein of Homo sapiens glucosidase, beta; acid (includes glucosylceramidase) (GBA), transcript variant 3, 20 µg |
CNY 2,900.00 |
|
| TP761812 | Purified recombinant protein of Human glucosidase, beta, acid (GBA), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug |
CNY 2,040.00 |
