Fig4 (NM_133999) Mouse Recombinant Protein
CAT#: TP511140
Purified recombinant protein of Mouse FIG4 phosphoinositide 5-phosphatase (Fig4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR211140 protein sequence
Red=Cloning site Green=Tags(s) MPTAAAPIISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVVIDDRHVYTQQEVRELLGRL DLGNRTKMSQKGSSGLFRAVSAFGVVGFVRFLEGYYIVLITKRRKMADIGGHAIYKIEDTSMIYIPNDSV RISHPDEARYLRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETSKACQESFDIFEDEGLI TQGGSGVFGISSEPYMKYVWNGELLDIIKNTVHRDWLLYIIHGFCGQSKLLIYGRPVYVTLIARRSSRFA GTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLFWSQDISTMMPKPPITLDQA DPFAHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAK YTKSKLCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGHVIPTGRLQTGILRTNCVDCL DRTNTAQFMVGKCALAYQLYSLGLIDKPNLQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKI APWTQHSKDIMQTLSRYYSNAFSDADRQDSINLFLGVFHPTEGKPHLWELPTDFYLHHKNTMSLLPPRRS YTYWWTPEVVKHLPLPYDEVICAANLKKLMVKKFHRWEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARD FMPKTVGIDPSPFTVRKPDETGKSVLGNKNTREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREDEG SISQRSTPVKMTDTGDSAKATENVVQPMKEVYGVSLSSSLSEEDHSIYARFVQLGQSQHKQDRGNQQLCS RCSDGVIKLTPISAFSQDNIYEVQPPRVDRKSTEIFQAHIQASQGIMQPLGKEDTAMYREYIRNRYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 103.4 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_598760 |
Locus ID | 103199 |
UniProt ID | Q91WF7 |
Refseq Size | 3278 |
Cytogenetics | 10 B1 |
Refseq ORF | 2724 |
Synonyms | A530089I17Rik; AI326867; Sac3 |
Summary | The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.[UniProtKB/Swiss-Prot Function] |
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