Arhgef2 (NM_008487) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211388 protein sequence Red=Cloning site Green=Tags(s) MSRIESLTRARIDRSKEQATKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPT CNVTIHNRCKDTLANCTKVKQKQQKAALLRNNTALQSVSLRSKTTTRERPTSAIYPSDSFRQSLLGSRRG LSSLSLAKSVSTTNIAGHFNDESPLGLRQILSQSTDSLNMRNRTLSVESLIDEGVEVFYNELMSDFEMDE KDFEADSWSLAVDSSFLQQHKKEVMKKQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGL FPCVDELSDIHTRFLNQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGSNAEQMRKTYSEFCSRHTKAL KLYKELYARDKRFQQFIRKMTRSAVLKRHGVQECILLVTQRITKYPVLINRILQNSHGVEEEYQDLASAL GLVKELLSNVDQDVHELEKEARLQEIYNRMDPRAQTPVPGKGPFGRDELLRRKLIHEGCLLWKTATGRFK DVLLLLMTDVLVFLQEKDQKYIFTSLDKPSVVSLQNLIVRDIANQAKGMFLISSGPPEMYEVHAASRDDR TTWIRVIQQSVRLCPSREDFPLIETEDKAYLRRIKTKLQQKNQALVELLQKNVELFAEMVHFQALKAGFV GMPPPALPRGLFRLESFESLRGERLLKDALREVEGLKDLLLGPCVDLPTTSREPALPLDSDSGSCPGVTA NGEARTFNGSIELCRADSDSSQKDRNGNQLRSPQEEVLQPLINLYGLLHGLQAVVVQQERLMEALFPEGP ERWEKLSRANSRDGEAGRAAVASVTPEKQATELALLQRQHTLLQEELRRCQRLGEERATEAGSLEARLRE SEQARALLEREAEEIRRQLAALGQNEPLPAEAPWARRPLDPRRRSLPAGDALYLSFNPPQPSRGHDRLDL PVTVRSLHRPFDDREAQELGSPEDRLQDSSDPDTGSEEEVSSRLSPPHSPRDFTRMQDIPEETESRDGEP TASES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	111.9 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_032513
Locus ID	16800
UniProt ID	Q60875
Refseq Size	4300
Cytogenetics	3 F1
Refseq ORF	2958
Synonyms	AA408978; GEF; GEF-H1; GEFH1; Lbcl1; Lfc; LFP40; mKIAA0651; P40
Summary	Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (PubMed:28453519).[UniProtKB/Swiss-Prot Function]

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