Helb (NM_080446) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211593 representing NM_080446 Red=Cloning site Green=Tags(s) MARQDRLRELLGPLHPYKSDDEEEDCAQEEEGEQEEEFVDAEELCSGGIKAGSLPGRARVSIPDEYTKEK CTVYGRFPLKGPWWRVKVQVLKPQRSRSYQVQGFPAYFLQVDMSPPDQKQICSLFLKECNLASERIQEFL KWVEKVSSFENLHFENLWETLRLFYRETEKKDKKLSTPREQQGEEMRVEKSFAFISAMVALQFPKVMEFL PSLFPRHFKRLISSSSDWVLGCIEDVLGTQPWKLGFRRITYREMKLVRCEASWTAFSQCPSLLQLMTPLQ KNALVIYSKLRQTCREDGHTYIEVKDLTSGLSEHMSFEEACQSLAFLKDIDVVIYEKDYVFLSELYEAEQ DIASSICELMSRPPWHLKVDVKNVLASIRGAKPNDPGSAEAVEGSKPEEVGSEQGDSVLDAQDGDDHVRS NGEHVANAEINDVPLDQDQVVALETICANAVTVLSGKGGCGKTTIVSRLFKHMEHLEETEVQQACEDFEQ DQEASEEWLDCPKQSPAGVDKAVEVLLTAPTGKAAGLLRQRTDLPAYTLCQVNYSFYMWKTKNEVDKPWK FSTVRVLVVDEGSLVSVGIFKSVLQLLCKHSKLSKLIILGDVRQLPSIEPGNMLQDVFETLKSRQCAIEL KTNHRTESQLIVDNATRISRRQFPKFDAELNICGNPTLPLSIQDKTFIFVRLPEEDSRSQSSKGEHRSNL YTAVKTLLQGKDFCSFESSKTSQFIAFRRQDCDLINDCCCKHYTGHLIKDHEKKLIFAVGDKICCTRNAY LSDLLPDKDQEAEGKGYGDAPDDDAKIKQDFESSTRLCNGEIFFITRDVTDVTFKRKRLLTINNEAGLEV TVDFSKLMANCQIKHAWARTIHTFQGSEENTVVYVVGKAGRQHWQHVYTAVTRGRSRVYIIAQESELRSA TRKRGFPRQTRLKHFLQKKLSGSCAPSTGFASQPSSPRVGGRPDTQPPASHLCRTPDNKATADSARGDER WLSASVNDDVDTDEESAQLRGSKRIGDGFPFDEESPSKFRMVEAPSPQVSSVFQNMRLNTLTPRQLFKPT DNQDTGTAGVADDANDPSNQEMEM SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	121.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_536694
Locus ID	117599
UniProt ID	Q6NVF4
Refseq Size	4540
Cytogenetics	10 67.94 cM
Refseq ORF	3222
Synonyms	AI447783; D10Ertd664e
Summary	5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed:26774285). Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285). Acts independently of TP53BP1 (PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress (PubMed:11557815, PubMed:7596831, PubMed:7794903).[UniProtKB/Swiss-Prot Function]

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