Plce1 (NM_019588) Mouse Recombinant Protein
CAT#: TP518818
Purified recombinant protein of Mouse phospholipase C, epsilon 1 (Plce1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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CNY 7,106.00
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR218818 representing NM_019588
Red=Cloning site Green=Tags(s) MTSEEMAASVLIPVTQRKVASAQSVAEERSVKVSDAGIPRARAGRQGALIPPTISQWNKHKEESSRSDLS KVFSIARGELVCDENSNEEGWEENAPDSPENHAMNGNSLVQSHQHQFPRSQLCEARDSVTEDPCLQPGIP SPLERKVLPGIQLEMEDSPMDVSPAGSQPRIMESSGPHSDRNTAVFHFHYEADRTMSDAFHTLSENLILD DCANCVTLPGGQQNKNCMAYACKLVELTRTCGSKNGQVQCEHCTSLRDEYLCFESSCSKADEVCSGGGFC EDGFAHGPAAKTFLNPLEDFSDNCEDVDDFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSG CIGPAAWNYVDQKKAGLLWPCGNVMGTLSAMDIRQSGSQRLSEAQWCLIYSAVRRGEEIEDTVGSLLHCS TQLPNSETAHGRIEDGPCLKQCVRDTECEFRATLQRTSIAQYITGSLLEATTSLGARSGLLSSFGGSTGR IMLKERQLGTSMANSNPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDL SSPECQRSLPRLKASISESILTSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAK CCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVV PFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNSEKELTVNSIFQV IRSCSRSLEMEEEDSASEGSGSRKNSLKDKARWQFIIGDLLDSENDIFEKSKECDPHGSEESQKAFDHGT ELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARPKLGVLSN MAEPGKFPSPGNAGVSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDN RLLHFVAPKHTAEMLFSGLLELTTAVRKIRRFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRR WSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDP QDVNEQEESEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIRGGMQGFQSFMVS DSNMSFVEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTSDLQPDLDLLTRNVS DLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSI IQKFEPSVSMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKELQLPLSYYYIESSHNTYLTGHQLKGE SSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHC SLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQL ASMQAQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKK ADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSSRGKERKSRKS IFGNNPGRMSPGETAPFNRTSGKGSCEGMRHTWEESSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCR RGSQKLIQHTAYQLLRTYPAATRIDSSNPNPIMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVL KPPVLWDKSCPMYQKFSPLERDLDNLDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKP IHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLRALKRGYRHLQLRNLHNEILEISS LFINSRRMEENPSGSSMPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQVLAVDQ DTKCTATDYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKS IVFDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLL EEVLKDAANKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLT KSTKQSRGLPSPPQLVASESVQSKEEKPVGALSSSDTVGYQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 255 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_062534 |
Locus ID | 74055 |
UniProt ID | Q8K4S1 |
Refseq Size | 9525 |
Cytogenetics | 19 C3 |
Refseq ORF | 6846 |
Synonyms | 4933403A21Rik; mKIAA1516; Plce; PLCepsilon |
Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.[UniProtKB/Swiss-Prot Function] |
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