Collagen IV (COL4A3) (NM_000091) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) 1        MALWIDRMQL LSCIALSLAL VTNSAGFVFT RHSQTTAIPS CPEGTVPLYS GFSFLFVQGN 61       QRAHGQDLGT LGSCLQRFTT MPFLFCNVND VCNFASRNDY SYWLSTPALM PMNMAPITGR 121      ALEPYISRCT VCEGPAIAIA VHSQTTDIPP CPHGWISLWK GFSFIMFTSA GSEGTGQALA 181      SPGSCLEEFR ASPFLECHGR GTCNYYSNSY SFWLASLNPE RMFRKPIPST VKAGELEKII 241      SRCQVCMKKR GGGGSDYKDD DDKHHHHHHH HHH
Tag	C-HIS/DDK
Predicted MW	27.5 kDa
Concentration	>0.05 µg/µL as determined by microplate Bradford method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	PBS, pH 7.4, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_000082
Locus ID	1285
UniProt ID	Q01955
Refseq Size	8050
Cytogenetics	2q36.3
Refseq ORF	5010
Synonyms	ATS2; ATS3
Summary	Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]
Protein Families	Druggable Genome

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.