Interferon gamma (IFNG) (NM_000619) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human IFN-gamma;, the region of Gln24-Gln166, from gene Accession# NM_000619
Tag	Tag Free
Predicted MW	17 kDa
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	20 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Recombinant human IFN-γ induced cytotoxic effect on HT-29 cells in a dose-dependent manner. The ED50 is 1 – 6 ng/mL.
Endotoxin	Less than 0.01 ng per ug protein as determined by the LAL method
Reference Data
RefSeq	NP_000610
Locus ID	3458
UniProt ID	P01579
Refseq Size	1240
Cytogenetics	12q15
Refseq ORF	498
Synonyms	IFG; IFI; IMD69
Summary	This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus

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