IL12A (NM_000882) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	Sf9
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Secreted protein p35: Accession# NM_000882, and p40: Accession# NM_002187
Tag	Tag Free
Predicted MW	57 kDa
Concentration	lot specific
Purity	95%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	Human IL-12 (p70) induces the production of IFNγ in human activated PBMC in a dose-dependent manner. The ED50 for this effect is 0.01 – 0.1 ng/mL.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_000873
Locus ID	3592
UniProt ID	P29459
Refseq Size	1450
Cytogenetics	3q25.33
Refseq ORF	759
Synonyms	CLMF; IL-12A; NFSK; NKSF1; P35
Summary	This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus

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