IL10 (NM_000572) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human IL-10, the region of Ser19-Asn178, from gene Accession# NM_000572
Tag	Tag Free
Predicted MW	18.6 kDa
Concentration	lot specific
Purity	>95%, as determined by Coomassie stained SDS-PAGE.
Buffer	1 x PBS
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.1 - 0.3 ng/ml corresponding to a specific activity of 3.3 - 10 x 10^6 units/mg, determined by the dose dependent inhibition of IFNg production by PHA activated PBMC.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_000563
Locus ID	3586
UniProt ID	P22301
Refseq Size	1629
Cytogenetics	1q32.1
Refseq ORF	534
Synonyms	CSIF; GVHDS; IL-10; IL10A; TGIF
Summary	The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway

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