PRSS3 (16-247, His-Tag) Human Recombinant Protein
CAT#: TP724592
Recombinant human Trypsin 3/PRSS3 protein
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CNY 1,819.00
货期*
5周
规格
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence |
VPFDDDDKIV GGYTCEENSL PYQVSLNSGS HFCGGSLISE QWVVSAAHCY KTRIQVRLGE HNIKVLEGNE QFINAAKIIR HPKYNRDTLD NDIMLIKLSS PAVINARVST ISLPTAPPAA GTECLISGWG NTLSFGADYP DELKCLDAPV LTQAECKASY PGKITNSMFC VGFLEGGKDS CQRDSGGPVV CNGQLQGVVS WGHGCAWKNR PGVYTKVYNY VDWIKDTIAA NS
|
Tag | His-Tag |
Predicted MW | 26kDa (238aa) |
Concentration | 1mg/ml (determined by Absorbance at 280nm) |
Purity | > 95% by SDS-PAGE |
Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Applications | SDS-PAGE, Enzyme Activity |
Storage | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Bioactivity | Specific activity is > 10,000pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 per minute at pH 8.0 at 37℃. |
Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Reference Data | |
RefSeq | NP_002762.2 |
Summary | PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. It has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins. Recombinant human Trypsin 3/PRSS3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
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