Perforin (PRF1) (NM_005041) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Asp351-End) of PRF1
Tag	N-His
Predicted MW	23.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	>80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	50 mM Tris-HCl, pH 8.0, 8 M urea
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_005032
Locus ID	5551
UniProt ID	P14222
Refseq Size	2512
Cytogenetics	10q22.1
Refseq ORF	1665
Synonyms	HPLH2; P1; PFP
Summary	This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
Protein Families	Druggable Genome
Protein Pathways	Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis

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