PD1 (PDCD1) Mouse Monoclonal Antibody [Clone ID: UMAB199]

Specifications

Product Data
Clone Name	UMAB199
Applications	10k-ChIP, IF, IHC, WB
Recommend Dilution	IHC 1:1000, WB 1:500, IF 1:100
Reactivity	Human, Mouse, Dog, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	29.2 kDa
Gene Name	programmed cell death 1
Database Link	NP_005009 Entrez Gene 18566 MouseEntrez Gene 301626 RatEntrez Gene 486213 DogEntrez Gene 5133 Human UniProt ID Q15116
Background	This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul
Synonyms	CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), T cell receptor signaling pathway

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