Atoh8 (NM_153778) Mouse Tagged Lenti ORF Clone
CAT#: MR204653L4
- LentiORF®
Lenti ORF clone of Atoh8 (mGFP-tagged) - Mouse atonal homolog 8 (Drosophila) (Atoh8)
"NM_153778" in other vectors (4)
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CNY 4,800.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 4933425C05Rik; bHLHa21; Hath6; Math6; okadin |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR204653).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153778 |
ORF Size | 969 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153778.3, NP_722473.1 |
RefSeq Size | 2355 bp |
RefSeq ORF | 969 bp |
Locus ID | 71093 |
UniProt ID | Q99NA2 |
Gene Summary | Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG- 3' known as an E-box element, possibly as a heterodimer with other bHLH proteins (By similarity). Regulates endothelial cell proliferation, migration and tube-like structures formation (By similarity). Modulates endothelial cell differentiation through NOS3 (By similarity). May be implicated in specification and differentiation of neuronal cell lineages in the brain (PubMed:11733035). May participate in kidney development and may be involved in podocyte differentiation (PubMed:16937370). During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3 (PubMed:18560595). During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase (PubMed:24186058). Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex (By similarity). Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif (PubMed:23938248).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204248 | Atoh8 (untagged) - Mouse atonal homolog 8 (Drosophila) (Atoh8), (10ug) |
CNY 2,400.00 |
|
MG204653 | Atoh8 (tGFP-tagged) - Mouse atonal homolog 8 (Drosophila) (Atoh8) |
CNY 2,850.00 |
|
MR204653 | Atoh8 (Myc-DDK-tagged) - Mouse atonal homolog 8 (Drosophila) (Atoh8) |
CNY 2,400.00 |
|
MR204653L3 | Lenti ORF clone of Atoh8 (Myc-DDK-tagged) - Mouse atonal homolog 8 (Drosophila) (Atoh8) |
CNY 4,750.00 |