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MAPK6 (NM_002748) Human Tagged ORF Clone
CAT＃: RC201597
MAPK6 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 6 (MAPK6)
ORF Plasmid: tGFP
"NM_002748" in other vectors (7)
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CNY 5,280.00
CNY 5,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ERK3; HsT17250; p97MAPK; PRKM6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGAAATTTGAAAGTCTCATGAACATTCATGGTTTTGATCTGGGTTCTAGGTATATGGACTTAA AACCATTGGGTTGTGGAGGCAATGGCTTGGTTTTTTCTGCTGTAGACAATGACTGTGACAAAAGAGTAGC CATCAAGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAACATGCTCTACGTGAAATCAAAATTATTAGA AGACTTGACCATGATAACATTGTGAAAGTGTTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACAGACG ATGTGGGCTCTCTTACGGAACTGAACAGTGTTTACATTGTTCAGGAGTACATGGAGACAGACTTGGCTAA TGTGCTGGAGCAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGGGGGCTC AAGTATATTCACTCTGCAAATGTACTGCACAGAGATCTCAAACCAGCTAATCTTTTCATTAATACGGAAG ACTTGGTGCTGAAGATAGGTGACTTTGGTCTTGCACGGATCATGGATCCTCATTATTCCCATAAGGGTCA TCTTTCTGAAGGATTGGTTACTAAATGGTACAGATCTCCACGTCTTTTACTTTCTCCTAATAATTATACT AAAGCCATTGACATGTGGGCTGCAGGCTGCATCTTTGCTGAAATGCTGACTGGTAAAACCCTTTTTGCAG GTGCACATGAACTTGAACAGATGCAGCTGATTTTAGAATCTATTCCTGTTGTACATGAGGAAGATCGTCA GGAGCTTCTCAGCGTAATTCCAGTTTACATTAGAAATGACATGACTGAGCCACACAAACCTTTAACTCAG CTGCTTCCAGGAATTAGTCGAGAAGCAGTGGATTTCCTGGAACAAATTTTGACATTTAGCCCCATGGATC GGTTAACAGCAGAAGAAGCACTCTCCCATCCTTACATGAGCATATATTCTTTTCCAATGGATGAGCCAAT TTCAAGCCATCCTTTTCATATTGAAGATGAAGTTGATGATATTTTGCTTATGGATGAAACTCACAGTCAC ATTTATAACTGGGAAAGGTATCATGATTGTCAGTTTTCAGAGCATGATTGGCCTGTACATAACAACTTTG ATATTGATGAAGTTCAGCTTGATCCAAGAGCTCTGTCCGATGTCACTGATGAAGAAGAAGTACAAGTTGA TCCCCGAAAATATTTGGATGGAGATCGGGAAAAGTATCTGGAGGATCCTGCTTTTGATACCAATTACTCT ACTGAGCCTTGTTGGCAATACTCAGATCATCATGAAAACAAATATTGTGATCTGGAGTGTAGCCATACTT GTAACTACAAAACGAGGTCATCATCATATTTAGATAACTTAGTTTGGAGAGAGAGTGAAGTTAACCATTA CTATGAACCCAAGCTTATTATAGATCTTTCCAATTGGAAAGAACAAAGCAAAGAAAAATCTGATAAGAAA GGCAAATCAAAATGTGAAAGGAATGGATTGGTTAAAGCCCAGATAGCGCTAGAGGAAGCATCACAGCAAC TGGCTGGAAAAGAAAGGGAAAAGAATCAGGGATTTGATTTTGATTCCTTTATTGCAGGAACTATTCAGCT TAGTTCCCAGCATGAGCCTACTGATGTTGTTGATAAATTAAATGACTTGAATAGCTCAGTGTCCCAACTA GAATTGAAAAGTTTGATATCAAAGTCAGTAAGCCAAGAAAAACAGGAAAAAGGAATGGCAAATCTGGCTC AATTAGAAGCCTTGTACCAGTCTTCTTGGGACAGCCAGTTTGTGAGTGGTGGGGAGGACTGTTTTTTCAT AAATCAGTTTTGTGAGGTAAGGAAGGATGAACAAGTTGAGAAGGAAAACACTTACACTAGTTACTTGGAC AAGTTCTTTAGCAGGAAAGAAGATACTGAAATGCTAGAAACTGAGCCAGTAGAGGATGGGAAGCTTGGGG AGAGAGGACATGAGGAAGGATTTCTGAACAACAGTGGGGAGTTCCTCTTTAACAAGCAGCTCGAGTCCAT AGGCATCCCACAGTTTCACAGTCCAGTTGGGTCACCACTTAAGTCAATACAGGCCACATTAACACCTTCT GCTATGAAATCTTCCCCTCAAATTCCTCATCAAACATACAGCAGCATTCTGAAACATCTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201597 protein sequence
Red=Cloning site Green=Tags(s) MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDKRVAIKKIVLTDPQSVKHALREIKIIR RLDHDNIVKVFEILGPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGL KYIHSANVLHRDLKPANLFINTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYT KAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLSVIPVYIRNDMTEPHKPLTQ LLPGISREAVDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPMDEPISSHPFHIEDEVDDILLMDETHSH IYNWERYHDCQFSEHDWPVHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYS TEPCWQYSDHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKK GKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVDKLNDLNSSVSQL ELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFINQFCEVRKDEQVEKENTYTSYLD KFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPS AMKSSPQIPHQTYSSILKHLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002748 |
| ORF Size | 2163 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002748.4 |
| RefSeq Size | 4225 bp |
| RefSeq ORF | 2166 bp |
| Locus ID | 5597 |
| UniProt ID | Q16659 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| MW | 82.7 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201597L1 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged |
CNY 7,680.00 |
|
| RC201597L2 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged |
CNY 7,700.00 |
|
| RC201597L3 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201597L4 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged |
CNY 7,700.00 |
|
| RG201597 | MAPK6 (tGFP-tagged) - Human mitogen-activated protein kinase 6 (MAPK6) |
CNY 6,370.00 |
|
| SC118465 | MAPK6 (untagged)-Human mitogen-activated protein kinase 6 (MAPK6) |
CNY 5,288.00 |
|
| SC323360 | MAPK6 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase 6 (MAPK6) |
CNY 5,288.00 |
