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TBK1 (NM_013254) Human Tagged ORF Clone
CAT＃: RC205238
TBK1 (Myc-DDK-tagged)-Human TANK-binding kinase 1 (TBK1)
ORF Plasmid: tGFP
"NM_013254" in other vectors (7)
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CNY 5,344.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FTDALS4; IIAE8; NAK; T2K |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205238 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTTAGGCCAAGGAGCTACTGCAAATGTCT TTCGTGGAAGACATAAGAAAACTGGTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCG TCCAGTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAAAAATATTGTCAAATTA TTTGCTATTGAAGAGGAGACAACAACAAGACATAAAGTACTTATTATGGAATTTTGTCCATGTGGGAGTT TATACACTGTTTTAGAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGTTTTGCG AGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAGTGCACCGTGATATCAAGCCAGGAAAT ATCATGCGTGTTATAGGGGAAGATGGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAAT TAGAAGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACCCTGATATGTATGAGAG AGCAGTGCTAAGAAAAGATCATCAGAAGAAATATGGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACA TTTTACCATGCAGCTACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAGAAGTGA TGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGAGTACAGAAAGCAGAAAATGGACCAAT TGACTGGAGTGGAGACATGCCTGTTTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTT CTTGCAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTTTTTGCAGAAACTAGTG ATATACTTCACCGAATGGTAATTCATGTTTTTTCGCTACAACAAATGACAGCTCATAAGATTTATATACA TAGCTATAATACTGCTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCAAATCAA GAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAGGCTGGCACAACATTTCCCTAAAACTA CTGAGGAAAACCCTATATTTGTAGTAAGCCGGGAACCTCTGGATACCATAGGATTAATATATGAAAAAAT TTCCCTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGCTAAGGCAATAACAGGG GTTGTGTGTTATGCCTGCAGAATTGCCAGTACCTTACTGCTTTATCAGGAATTAATGCGAAAGGGGATAC GATGGCTGATTGAATTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGTGATCAC ATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTATATGAAAAGTTGATGAAGATCAACCTG GAAGCGGCAGAGTTAGGTGAAATTTCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAA CAATAGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCACTGGCAGACGCATGGGC ACATCAAGAAGGCACTCATCCGAAAGACAGAAATGTAGAAAAACTACAAGTCCTGTTAAATTGCATGACA GAGATTTACTATCAGTTCAAAAAAGACCAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAATCCACA AATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATGACGCACTTTACAGATGAATGTGTTAA AAAGTATGAGGCATTTTTGAATAAGTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTA TTATCGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAAGAATATACTAATGAGT TACAAGAAACTCTGCCTCAGAAAATGTTTACAGCTTCCAGTGGAATCAAACATACCATGACCCCAATTTA TCCAAGTTCTAACACATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAAGGGGTG GTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGGCTCTTTAACCATGGATGGTGGCCTTC GCAACGTTGACTGTCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205238 protein sequence
Red=Cloning site Green=Tags(s) MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKL FAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGN IMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVT FYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPV LANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQ ELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLDTIGLIYEKISLPKVHPRYDLDGDASMAKAITG VVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINL EAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMT EIYYQFKKDQAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQL LSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGV VKELAENNHILERFGSLTMDGGLRNVDCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013254 |
| ORF Size | 2187 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013254.4 |
| RefSeq Size | 3098 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 29110 |
| UniProt ID | Q9UHD2 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway |
| MW | 83.6 kDa |
| Gene Summary | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TBK1 phosphorylates mutant Huntingtin and suppresses its aggregation and toxicity in Huntington\'s disease models
,Hegde, RN;Chiki, A;Petricca, L;Martufi, P;Arbez, N;Mouchiroud, L;Auwerx, J;Landles, C;Bates, GP;Singh-Bains, MK;Dragunow, M;Curtis, MA;Faull, RL;Ross, CA;Caricasole, A;Lashuel, HA;,
EMBO J.
,PubMed ID 32757223
[TBK1]
|
|
MiR-221 negatively regulates innate anti-viral response
,Du, H;Cui, S;Li, Y;Yang, G;Wang, P;Fikrig, E;You, F;,
PLoS ONE
,PubMed ID 30089112
[TBK1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205238L1 | Lenti ORF clone of Human TANK-binding kinase 1 (TBK1), Myc-DDK-tagged |
CNY 7,744.00 |
|
| RC205238L2 | Lenti ORF clone of Human TANK-binding kinase 1 (TBK1), mGFP tagged |
CNY 7,744.00 |
|
| RC205238L3 | Lenti ORF clone of Human TANK-binding kinase 1 (TBK1), Myc-DDK-tagged |
CNY 7,744.00 |
|
| RC205238L4 | Lenti ORF clone of Human TANK-binding kinase 1 (TBK1), mGFP tagged |
CNY 7,744.00 |
|
| RG205238 | TBK1 (tGFP-tagged) - Human TANK-binding kinase 1 (TBK1) |
CNY 6,944.00 |
|
| SC111251 | TBK1 (untagged)-Human TANK-binding kinase 1 (TBK1) |
CNY 5,344.00 |
|
| SC323338 | TBK1 (untagged)-Kinase deficient mutant (K38M) of Human TANK-binding kinase 1 (TBK1) |
CNY 5,344.00 |
