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SECISBP2 (NM_024077) Human Tagged ORF Clone
CAT＃: RC206346
SECISBP2 (Myc-DDK-tagged)-Human SECIS binding protein 2 (SECISBP2)
ORF Plasmid: tGFP
"NM_024077" in other vectors (6)
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CNY 6,256.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SBP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206346 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGAGGGGCCGCGGGAGCCCGAAAGCGAGGGCATCAAGTTATCAGCAGATGTCAAACCATTTG TCCCCAGATTTGCCGGGCTCAATGTGGCATGGTTAGAGTCCTCAGAAGCATGTGTCTTCCCCAGCTCTGC AGCCACATACTATCCGTTTGTTCAGGAACCACCAGTGACAGAGCAGAAAATATATACTGAAGACATGGCC TTTGGAGCTTCAACTTTTCCACCTCAGTATTTATCTTCTGAGATAACTCTTCATCCATATGCCTATTCTC CTTATACCCTTGACTCCACACAGAATGTTTACTCAGTGCCTGGCTCCCAGTATCTTTATAACCAACCCAG TTGTTACCGAGGTTTTCAAACAGTGAAGCATCGAAATGAGAACACATGCCCTCTCCCACAAGAAATGAAA GCTCTGTTTAAGAAGAAAACCTATGATGAGAAAAAAACGTATGATCAGCAAAAGTTTGACAGTGAAAGGG CTGATGGAACTATATCATCTGAGATAAAATCAGCTAGAGGTTCACATCATTTGTCCATTTACGCTGAGAA TAGTTTGAAATCAGATGGTTACCATAAGCGAACAGACAGGAAATCCAGAATCATTGCAAAAAATGTATCT ACCTCCAAACCTGAGTTTGAATTTACCACACTGGACTTTCCTGAACTGCAAGGTGCAGAGAACAATATGT CAGAGATACAGAAGCAACCCAAGTGGGGACCTGTCCACTCTGTCTCTACCGACATTTCTCTTCTAAGAGA AGTAGTAAAACCAGCTGCAGTGTTATCAAAGGGTGAAATAGTGGTGAAAAATAACCCAAATGAATCTGTA ACTGCTAATGCCGCTACCAATTCTCCTTCATGTACAAGAGAGTTATCTTGGACACCAATGGGTTATGTTG TTCGACAGACATTATCTACAGAACTGTCAGCAGCCCCTAAAAATGTTACTTCTATGATAAACTTAAAGAC CATTGCTTCATCAGCAGATCCTAAAAATGTTAGTATACCATCTTCTGAAGCTTTATCTTCGGATCCTTCC TACAACAAAGAAAAACACATTATTCATCCTACCCAAAAGTCTAAAGCATCACAAGGTAGTGACCTTGAAC AAAATGAAGCCTCAAGAAAGAATAAGAAAAAGAAAGAAAAATCTACATCAAAATATGAAGTCCTGACAGT TCAAGAGCCTCCAAGGATTGAAGATGCCGAGGAATTTCCCAACCTGGCAGTTGCATCTGAAAGAAGAGAC AGAATAGAGACACCGAAATTTCAATCTAAGCAGCAGCCACAGGATAATTTTAAAAATAATGTAAAGAAGA GCCAGCTTCCAGTGCAGTTGGACTTGGGGGGCATGCTGACAGCCCTGGAGAAGAAGCAGCACTCTCAGCA TGCAAAGCAGTCCTCCAAACCAGTGGTAGTCTCAGTTGGAGCAGTGCCAGTCCTTTCCAAAGAATGTGCA TCAGGGGAGAGAGGCCGCCGCATGAGTCAAATGAAGACCCCGCACAATCCCTTGGACTCCAGCGCCCCAC TGATGAAGAAAGGGAAGCAGAGGGAGATCCCCAAGGCCAAGAAGCCAACCTCACTGAAGAAGATTATTTT GAAAGAACGGCAAGAGAGAAAGCAGCGTCTCCAAGAAAATGCTGTGGGTCCAGCTTTTACCAGTGATGAC ACACAAGATGGAGAGAGTGGTGGTGATGACCAGTTTCCCGAGCAGGCAGAGCTGTCAGGGCCAGAGGGGA TGGACGAACTGATCTCCACTCCTTCGGTTGAGGACAAGTCTGAAGAGCCACCAGGCACAGAGCTCCAGAG GGACACAGAGGCCTCCCACCTTGCTCCCAATCACACCACCTTCCCTAAGATCCACAGCCGCAGATTCAGG GATTACTGCAGCCAGATGCTTAGTAAAGAAGTGGATGCTTGTGTTACCGACCTACTCAAAGAACTGGTCC GTTTCCAAGACCGTATGTACCAGAAAGATCCAGTCAAGGCCAAGACTAAACGTCGACTTGTGTTGGGGTT GAGGGAGGTTCTCAAACACCTGAAGCTCAAAAAACTGAAATGTGTCATTATTTCTCCCAACTGTGAGAAG ATACAGTCAAAAGGTGGGCTGGATGACACTTTGCACACAATTATTGATTATGCCTGTGAGCAGAACATTC CCTTTGTGTTTGCTCTCAACCGCAAAGCTCTGGGGCGCAGTTTGAATAAGGCAGTTCCTGTCAGTGTGGT GGGGATCTTCAGCTATGATGGGGCCCAGGATCAGTTCCACAAGATGGTTGAGCTGACAGTGGCGGCCCGA CAGGCGTACAAGACCATGCTGGAGAATGTGCAGCAGGAGCTGGTGGGAGAGCCCAGGCCTCAGGCACCTC CCAGCCTACCCACACAGGGCCCCAGCTGCCCTGCAGAAGATGGCCCCCCAGCCCTGAAAGAAAAAGAAGA GCCACACTACATTGAAATCTGGAAAAAACATCTGGAAGCATACAGTGGATGTACCCTGGAGCTAGAAGAA TCCTTGGAGGCTTCAACCTCTCAAATGATGAATTTGAATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206346 protein sequence
Red=Cloning site Green=Tags(s) MASEGPREPESEGIKLSADVKPFVPRFAGLNVAWLESSEACVFPSSAATYYPFVQEPPVTEQKIYTEDMA FGASTFPPQYLSSEITLHPYAYSPYTLDSTQNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTCPLPQEMK ALFKKKTYDEKKTYDQQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRIIAKNVS TSKPEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESV TANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPS YNKEKHIIHPTQKSKASQGSDLEQNEASRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRD RIETPKFQSKQQPQDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECA SGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVGPAFTSDD TQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFR DYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEK IQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAAR QAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTLELEE SLEASTSQMMNLNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024077 |
| ORF Size | 2562 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024077.5 |
| RefSeq Size | 3535 bp |
| RefSeq ORF | 2565 bp |
| Locus ID | 79048 |
| UniProt ID | Q96T21 |
| Domains | Ribosomal_L7Ae |
| MW | 95.4 kDa |
| Gene Summary | The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206346L1 | Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), Myc-DDK-tagged |
CNY 8,656.00 |
|
| RC206346L2 | Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), mGFP tagged |
CNY 9,030.00 |
|
| RC206346L3 | Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC206346L4 | Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), mGFP tagged |
CNY 9,030.00 |
|
| RG206346 | SECISBP2 (tGFP-tagged) - Human SECIS binding protein 2 (SECISBP2) |
CNY 7,856.00 |
|
| SC112316 | SECISBP2 (untagged)-Human SECIS binding protein 2 (SECISBP2) |
CNY 6,264.00 |
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