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IDO1 (NM_002164) Human Tagged ORF Clone
CAT＃: RC206592
IDO1 (Myc-DDK-tagged)-Human indoleamine 2,3-dioxygenase 1 (IDO1)
ORF Plasmid: tGFP
"NM_002164" in other vectors (6)
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CNY 5,488.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IDO; IDO-1; INDO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206592 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACACGCTATGGAAAACTCCTGGACAATCAGTAAAGAGTACCATATTGATGAAGAAGTGGGCTTTG CTCTGCCAAATCCACAGGAAAATCTACCTGATTTTTATAATGACTGGATGTTCATTGCTAAACATCTGCC TGATCTCATAGAGTCTGGCCAGCTTCGAGAAAGAGTTGAGAAGTTAAACATGCTCAGCATTGATCATCTC ACAGACCACAAGTCACAGCGCCTTGCACGTCTAGTTCTGGGATGCATCACCATGGCATATGTGTGGGGCA AAGGTCATGGAGATGTCCGTAAGGTCTTGCCAAGAAATATTGCTGTTCCTTACTGCCAACTCTCCAAGAA ACTGGAACTGCCTCCTATTTTGGTTTATGCAGACTGTGTCTTGGCAAACTGGAAGAAAAAGGATCCTAAT AAGCCCCTGACTTATGAGAACATGGACGTTTTGTTCTCATTTCGTGATGGAGACTGCAGTAAAGGATTCT TCCTGGTCTCTCTATTGGTGGAAATAGCAGCTGCTTCTGCAATCAAAGTAATTCCTACTGTATTCAAGGC AATGCAAATGCAAGAACGGGACACTTTGCTAAAGGCGCTGTTGGAAATAGCTTCTTGCTTGGAGAAAGCC CTTCAAGTGTTTCACCAAATCCACGATCATGTGAACCCAAAAGCATTTTTCAGTGTTCTTCGCATATATT TGTCTGGCTGGAAAGGCAACCCCCAGCTATCAGACGGTCTGGTGTATGAAGGGTTCTGGGAAGACCCAAA GGAGTTTGCAGGGGGCAGTGCAGGCCAAAGCAGCGTCTTTCAGTGCTTTGACGTCCTGCTGGGCATCCAG CAGACTGCTGGTGGAGGACATGCTGCTCAGTTCCTCCAGGACATGAGAAGATATATGCCACCAGCTCACA GGAACTTCCTGTGCTCATTAGAGTCAAATCCCTCAGTCCGTGAGTTTGTCCTTTCAAAAGGTGATGCTGG CCTGCGGGAAGCTTATGACGCCTGTGTGAAAGCTCTGGTCTCCCTGAGGAGCTACCATCTGCAAATCGTG ACTAAGTACATCCTGATTCCTGCAAGCCAGCAGCCAAAGGAGAATAAGACCTCTGAAGACCCTTCAAAAC TGGAAGCCAAAGGAACTGGAGGCACTGATTTAATGAATTTCCTGAAGACTGTAAGAAGTACAACTGAGAA ATCCCTTTTGAAGGAAGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206592 protein sequence
Red=Cloning site Green=Tags(s) MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHL TDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPN KPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKA LQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQ QTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002164 |
| ORF Size | 1209 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002164.6 |
| RefSeq Size | 1944 bp |
| RefSeq ORF | 1212 bp |
| Locus ID | 3620 |
| UniProt ID | P14902 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Tryptophan metabolism |
| MW | 45.3 kDa |
| Gene Summary | This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Structure-based optimization of type III indoleamine 2,3-dioxygenase 1 (IDO1) inhibitors
,null,
Journal of Enzyme Inhibition and Medicinal Chemistry
,PubMed ID 35758198
[IDO1]
|
|
Subversion of serotonin-receptor signaling in osteoblasts by kynurenine drives Acute Myeloid Leukemia
,null,
Cancer discovery
,PubMed ID 35046097
[IDO1]
|
|
Azole-Based Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors
,Röhrig, UF;Majjigapu, SR;Reynaud, A;Pojer, F;Dilek, N;Reichenbach, P;Ascencao, K;Irving, M;Coukos, G;Vogel, P;Michielin, O;Zoete, V;,
Journal of medicinal chemistry
,PubMed ID 33557523
[IDO1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206592L1 | Lenti ORF clone of Human indoleamine 2,3-dioxygenase 1 (IDO1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206592L2 | Lenti ORF clone of Human indoleamine 2,3-dioxygenase 1 (IDO1), mGFP tagged |
CNY 7,888.00 |
|
| RC206592L3 | Lenti ORF clone of Human indoleamine 2,3-dioxygenase 1 (IDO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206592L4 | Lenti ORF clone of Human indoleamine 2,3-dioxygenase 1 (IDO1), mGFP tagged |
CNY 7,888.00 |
|
| RG206592 | IDO1 (tGFP-tagged) - Human indoleamine 2,3-dioxygenase 1 (IDO1) |
CNY 7,088.00 |
|
| SC126221 | IDO1 (untagged)-Human indoleamine 2,3-dioxygenase 1 (IDO1) |
CNY 5,488.00 |
