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AKNA (NM_030767) Human Tagged ORF Clone
CAT＃: RC208832
- TrueORF®
AKNA (Myc-DDK-tagged)-Human AT-hook transcription factor (AKNA)
ORF Plasmid: tGFP
"NM_030767" in other vectors (4)
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CNY 9,848.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208832 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCGGAGACTGAGATCCGCTGGGCTGAGCCTGGCCTGGGGAAGGGCCCCCAGCGGCGGCGCT GGGCCTGGGCCGAGGACAAGAGGGATGTGGATAGAAGTAGTTCACAAAGCTGGGAAGAAGAGAGACTCTT TCCCAATGCCACCAGCCCCGAGCTCCTAGAGGACTTCCGCCTGGCCCAGCAGCACCTGCCGCCCCTGGAG TGGGACCCACACCCGCAGCCCGATGGGCATCAGGATTCCGAGTCAGGAGAGACTTCGGGAGAAGAGGCTG AAGCAGAGGATGTGGACAGCCCAGCAAGTTCCCATGAGCCTCTTGCCTGGCTCCCCCAGCAGGGCCGTCA GCTGGACATGACTGAAGAGGAGCCAGATGGGACCCTCGGAAGTCTGGAGGTTGAGGAGGCTGGAGAGAGC TCCTCAAGGTTGGGGTATGAGGCTGGTCTCAGCTTGGAAGGCCATGGAAACACCAGCCCCATGGCTCTTG GGCATGGTCAGGCCAGGGGCTGGGTGGCTTCTGGCGAACAAGCCAGTGGGGACAAACTTTCTGAACATTC CGAGGTCAACCCATCCGTTGAACTCAGCCCGGCAAGGTCCTGGAGCAGTGGGACAGTGAGCCTCGACCAC CCTAGTGACAGCCTTGATTCTACCTGGGAAGGAGAGACCGATGGCCCCCAGCCCACTGCCCTGGCAGAAA CCTTGCCAGAGGGCCCCAGCCACCACCTCCTAAGCCCAGATGGCAGAACTGGAGGCAGTGTTGCTCGGGC AACCCCCATGGAATTCCAGGACTCCTCAGCTCCCCCAGCCCAGAGTCCGCAGCATGCCACAGATAGATGG AGGAGAGAAACGACCAGATTCTTCTGCCCTCAGCCCAAGGAACACATCTGGAAGCAGACAAAGACGTCAC CTAAGCCACTCCCTTCCCGATTCATTGGCTCCATCAGCCCCCTGAATCCCCAGCCCAGGCCAACGCGGCA GGGCAGGCCGCTGCCCAGACAGGGAGCCACTCTGGCTGGCCGCTCCTCTTCTAATGCCCCCAAGTATGGC CGGGGGCAGTTGAACTACCCACTCCCTGATTTCTCCAAGGTAGGGCCCCGGGTGAGATTCCCCAAAGATG AGAGCTACCGTCCCCCCAAGTCCAGAAGCCACAACAGGAAGCCTCAGGCCCCTGCCAGGCCCCTCATCTT CAAGTCTCCAGCTGAGATTGTGCAGGAGGTGCTGTTGAGCAGTGGAGAAGCAGCCCTGGCAAAGGACACG CCTCCTGCCCACCCTATCACCAGGGTACCCCAAGAATTTCAGACGCCTGAGCAAGCCACTGAGCTGGTCC ATCAGCTCCAGGAAGACTACCACAGGCTCCTCACCAAGTACGCTGAGGCCGAGAACACCATTGACCAGCT ACGCCTCGGGGCCAAGGTGAACCTGTTCTCTGACCCACCCCAGCCCAACCACAGCATCCACACGGGAATG GTGCCCCAGGGGACCAAGGTCTTGTCCTTCACCATCCCACAGCCCCGCTCTGCAGAGTGGTGGCCGGGCC CGGCCGAGGACCCCCAGGCCTCTGCGGCCTCAGGGTGGCCATCAGCTCGAGGAGACTTGAGCCCCTCCTC GCTTACCAGCATGCCCACCCTGGGGTGGCTTCCGGAGAACCGGGACATCTCTGAGGACCAGTCCTCAGCA GAGCAGACCCAGGCACTGGCTTCTCAGGCCAGCCAGTTCCTGGCCAAGGTGGAGTCCTTTGAAAGACTGA TACAGGCAGGACGTCTCATGCCCCAGGACCAAGTCAAGGGCTTCCAGCGGCTGAAGGCTGCCCACGCGGC CCTAGAGGAGGAGTACCTGAAGGCTTGTCGGGAGCAACACCCTGCCCAGCCGCTTGCCGGCTCCAAGGGG ACGCCTGGAAGATTTGATCCTCGCAGGGAGCTGGAGGCAGAGATATACCGTCTGGGAAGCTGCCTGGAAG AGCTGAAGGAACACATAGACCAGACCCAGCAAGAGCCTGAGCCGCCCGGGTCAGACTCAGCTCTGGACAG CACCCCAGCCCTGCCCTGCCTCCATCAGCCAACGCACCTGCCTGCTCCTTCTGGACAAGCCCCCATGCCA GCCATCAAGACCTCCTGCCCTGAGCCTGCTACCACCACTGCCGCCGCCAGCACTGGCCCCTGCCCATTGC ACGTAAATGTGGAGGTGAGCTCTGGCAACAGTGAGGTGGAGGACAGGCCACAGGACCCCCTGGCCCGACT CAGGCACAAGGAGCTGCAGATGGAGCAAGTTTACCATGGCCTCATGGAGCGGTACCTCAGTGTGAAGTCT CTCCCAGAAGCCATGAGAATGGAGGAGGAGGAAGAAGGAGAGGAGGAGGAGGAGGAAGAGGGGGGAGGTG ACTCCCTGGAAGTTGATGGGGTGGCTGCAACTCCAGGGAAAGCAGAGGCCACCAGGGTCCTCCCAAGGCA GTGCCCGGTGCAGGCTGAGAAAAGTCATGGGGCTCCCCTGGAGGAGGCCACGGAGAAGATGGTATCTATG AAGCCACCAGGTTTCCAGGCATCCCTGGCTAGAGACGGGCACATGTCAGGCCTGGGCAAGGCTGAGGCAG CCCCTCCAGGCCCTGGCGTGCCACCCCACCCTCCAGGCACCAAGTCCGCAGCATCCCACCAAAGTAGTAT GACCAGCCTGGAGGGAAGCGGCATCTCTGAGCGCCTTCCACAGAAGCCTTTGCACCGAGGCGGTGGGCCC CACCTGGAGGAGACCTGGATGGCGTCCCCAGAGACAGACAGTGGCTTTGTGGGCTCAGAAACAAGCAGAG TTTCACCCCTCACCCAGACTCCAGAGCACCGGCTCTCCCACATCAGCACAGCAGGAACATTAGCCCAGCC CTTTGCTGCATCTGTGCCCAGGGATGGAGCTTCCTACCCCAAGGCCAGGGGTTCTCTGATTCCCAGAAGA GCCACAGAGCCCAGCACACCCCGGAGCCAAGCACAGAGGTACCTCTCCAGCCCAAGTGGGCCTCTCCGGC AGAGGGCACCCAACTTCAGCCTGGAGCGGACACTGGCAGCCGAGATGGCGGTTCCTGGCTCAGAGTTTGA GGGGCACAAACGGATTTCTGAACAGCCCCTTCCCAACAAGACAATCAGCCCACCCCCAGCCCCCGCCCCT GCCGCTGCGCCTCTACCCTGTGGACCAACAGAGACCATCCCCAGCTTCCTGCTCACCAGGGCAGGGCGAG ACCAGGCCATCTGTGAGCTGCAAGAAGAGGTGTCCCGGCTTCGTCTGCGGCTGGAAGACAGCCTGCACCA GCCACTCCAGGGCAGCCCGACACGCCCAGCATCTGCCTTTGACCGCCCCGCCCGGACCCGCGGCCGGCCA GCAGACTCCCCAGCCACCTGGGGCTCCCATTATGGCAGTAAATCCACAGAGAGATTGCCTGGTGAGCCTA GAGGTGAAGAGCAGATTGTCCCTCCAGGAAGGCAGCGAGCCAGGTCTTCCTCAGTGCCTCGGGAGGTGCT CCGACTGTCCCTGAGTTCAGAATCTGAGCTGCCCTCCCTACCACTGTTCTCTGAGAAGAGCAAGACCACC AAGGACAGTCCACAGGCAGCTCGGGATGGAAAGAGAGGGGTGGGCAGTGCTGGATGGCCAGACAGGGTCA CCTTCCGGGGCCAATACACAGGCCACGAATACCATGTTCTGTCCCCTAAGGCGGTCCCAAAAGGCAATGG CACAGTCTCCTGTCCCCACTGCCGGCCCATTAGGACCCAGGATGCGGGTGGTGCTGTCACAGGGGACCCA CTGGGACCGCCTCCCGCTGATACCCTTCAGTGTCCCCTGTGTGGTCAAGTTGGGTCTCCCCCAGAGGCAG ATGGTCCAGGCTCAGCCACCTCTGGGGCAGAGAAGGCCACCACGAGGAGAAAAGCACCTTCAACTCCCAG CCCCAAGCAGAGGAGCAAGCAGGCGGGGTCGTCGCCACGCCCACCCCCCGGACTGTGGTATCTGGCAACA GCGCCCCCAGCACCAGCCCCTCCAGCCTTTGCCTACATCTCCTCGGTTCCCATCATGCCTTATCCACCTG CCGCTGTGTACTATGCGCCTGCAGGACCTACCTCAGCCCAACCAGCTGCCAAGTGGCCGCCCACAGCCTC TCCCCCACCAGCCCGGAGACACCGGCACTCCATCCAGCTCGACCTGGGCGACCTAGAGGAGCTCAACAAG GCCCTGAGCCGGGCCGTGCAGGCTGCCGAGAGCGTCCGCTCTACCACCAGGCAGATGAGAAGCTCGCTGT CAGCCGACCTGCGCCAGGCTCACAGCCTGCGGGGCTCCTGCCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208832 protein sequence
Red=Cloning site Green=Tags(s) MASSETEIRWAEPGLGKGPQRRRWAWAEDKRDVDRSSSQSWEEERLFPNATSPELLEDFRLAQQHLPPLE WDPHPQPDGHQDSESGETSGEEAEAEDVDSPASSHEPLAWLPQQGRQLDMTEEEPDGTLGSLEVEEAGES SSRLGYEAGLSLEGHGNTSPMALGHGQARGWVASGEQASGDKLSEHSEVNPSVELSPARSWSSGTVSLDH PSDSLDSTWEGETDGPQPTALAETLPEGPSHHLLSPDGRTGGSVARATPMEFQDSSAPPAQSPQHATDRW RRETTRFFCPQPKEHIWKQTKTSPKPLPSRFIGSISPLNPQPRPTRQGRPLPRQGATLAGRSSSNAPKYG RGQLNYPLPDFSKVGPRVRFPKDESYRPPKSRSHNRKPQAPARPLIFKSPAEIVQEVLLSSGEAALAKDT PPAHPITRVPQEFQTPEQATELVHQLQEDYHRLLTKYAEAENTIDQLRLGAKVNLFSDPPQPNHSIHTGM VPQGTKVLSFTIPQPRSAEWWPGPAEDPQASAASGWPSARGDLSPSSLTSMPTLGWLPENRDISEDQSSA EQTQALASQASQFLAKVESFERLIQAGRLMPQDQVKGFQRLKAAHAALEEEYLKACREQHPAQPLAGSKG TPGRFDPRRELEAEIYRLGSCLEELKEHIDQTQQEPEPPGSDSALDSTPALPCLHQPTHLPAPSGQAPMP AIKTSCPEPATTTAAASTGPCPLHVNVEVSSGNSEVEDRPQDPLARLRHKELQMEQVYHGLMERYLSVKS LPEAMRMEEEEEGEEEEEEEGGGDSLEVDGVAATPGKAEATRVLPRQCPVQAEKSHGAPLEEATEKMVSM KPPGFQASLARDGHMSGLGKAEAAPPGPGVPPHPPGTKSAASHQSSMTSLEGSGISERLPQKPLHRGGGP HLEETWMASPETDSGFVGSETSRVSPLTQTPEHRLSHISTAGTLAQPFAASVPRDGASYPKARGSLIPRR ATEPSTPRSQAQRYLSSPSGPLRQRAPNFSLERTLAAEMAVPGSEFEGHKRISEQPLPNKTISPPPAPAP AAAPLPCGPTETIPSFLLTRAGRDQAICELQEEVSRLRLRLEDSLHQPLQGSPTRPASAFDRPARTRGRP ADSPATWGSHYGSKSTERLPGEPRGEEQIVPPGRQRARSSSVPREVLRLSLSSESELPSLPLFSEKSKTT KDSPQAARDGKRGVGSAGWPDRVTFRGQYTGHEYHVLSPKAVPKGNGTVSCPHCRPIRTQDAGGAVTGDP LGPPPADTLQCPLCGQVGSPPEADGPGSATSGAEKATTRRKAPSTPSPKQRSKQAGSSPRPPPGLWYLAT APPAPAPPAFAYISSVPIMPYPPAAVYYAPAGPTSAQPAAKWPPTASPPPARRHRHSIQLDLGDLEELNK ALSRAVQAAESVRSTTRQMRSSLSADLRQAHSLRGSCLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030767 |
| ORF Size | 4317 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030767.2, NP_110394.2 |
| RefSeq Size | 7387 bp |
| RefSeq ORF | 4320 bp |
| Locus ID | 80709 |
| UniProt ID | Q7Z591 |
| MW | 155.1 kDa |
| Gene Summary | Centrosomal protein that plays a key role in cell delamination by regulating microtubule organization (By similarity). Required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis (By similarity). Also regulates the epithelial-to-mesenchymal transition in other epithelial cells (By similarity). Acts by increasing centrosomal microtubule nucleation and recruiting nucleation factors and minus-end stabilizers, thereby destabilizing microtubules at the adherens junctions and mediating constriction of the apical endfoot (By similarity). In addition, may also act as a transcription factor that specifically activates the expression of the CD40 receptor and its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for antigen-dependent-B-cell development (PubMed:11268217). Binds to A/T-rich promoters (PubMed:11268217). It is unclear how it can both act as a microtubule organizer and as a transcription factor; additional evidences are required to reconcile these two apparently contradictory functions (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208832L3 | Lenti ORF clone of Human AT-hook transcription factor (AKNA), Myc-DDK-tagged |
CNY 16,250.00 |
|
| RC208832L4 | Lenti ORF clone of Human AT-hook transcription factor (AKNA), mGFP tagged |
CNY 16,250.00 |
|
| RG208832 | AKNA (tGFP-tagged) - Human AT-hook transcription factor (AKNA) |
CNY 11,448.00 |
|
| SC311505 | AKNA (untagged)-Human AT-hook transcription factor (AKNA) |
CNY 9,856.00 |
