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SCUBE2 (NM_020974) Human Tagged ORF Clone
CAT＃: RG224136
- TrueORF®
SCUBE2 (tGFP-tagged) - Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 1
ORF Plasmid: DDK
"NM_020974" in other vectors (4)
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CNY 9,648.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CEGB1; CEGF1; CEGP1; scube/You |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224136 representing NM_020974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGCCTGGGCGGTGCTGCTGCTGCTGCTGCTGCTGCCGC CACTGCTGCTGCTGGCGGGGGCCGTCCCGCCGGGTCGGGGCCGTGCCGCGGGGCCGCAGGAGGATGTAGA TGAGTGTGCCCAAGGGCTAGATGACTGCCATGCCGACGCCCTGTGTCAGAACACACCCACCTCCTACAAG TGCTCCTGCAAGCCTGGCTACCAAGGGGAAGGCAGGCAGTGTGAGGACATCGATGAATGTGGAAATGAGC TCAATGGAGGCTGTGTCCATGACTGTTTGAATATTCCAGGCAATTATCGTTGCACTTGTTTTGATGGCTT CATGTTGGCTCATGACGGTCATAATTGTCTTGATGTGGACGAGTGCCTGGAGAACAATGGCGGCTGCCAG CATACCTGTGTCAACGTCATGGGGAGCTATGAGTGCTGCTGCAAGGAGGGGTTTTTCCTGAGTGACAATC AGCACACCTGCATTCACCGCTCGGAAGAGGGCCTGAGCTGCATGAATAAGGATCACGGCTGTAGTCACAT CTGCAAGGAGGCCCCAAGGGGCAGCGTCGCCTGTGAGTGCAGGCCTGGTTTTGAGCTGGCCAAGAACCAG AGAGACTGCATCTTGACCTGTAACCATGGGAACGGTGGGTGCCAGCACTCCTGTGACGATACAGCCGATG GCCCAGAGTGCAGCTGCCATCCACAGTACAAGATGCACACAGATGGGAGGAGCTGCCTTGAGCGAGAGGA CACTGTCCTGGAGGTGACAGAGAGCAACACCACATCAGTGGTGGATGGGGATAAACGGGTGAAACGGCGG CTGCTCATGGAAACGTGTGCTGTCAACAATGGAGGCTGTGACCGCACCTGTAAGGATACTTCGACAGGTG TCCACTGCAGTTGTCCTGTTGGATTCACTCTCCAGTTGGATGGGAAGACATGTAAAGATATTGATGAGTG CCAGACCCGCAATGGAGGTTGTGATCATTTCTGCAAAAACATCGTGGGCAGTTTTGACTGCGGCTGCAAG AAAGGATTTAAATTATTAACAGATGAGAAGTCTTGCCAAGATGTGGATGAGTGCTCTTTGGATAGGACCT GTGACCACAGCTGCATCAACCACCCTGGCACATTTGCTTGTGCTTGCAACCGAGGGTACACCCTGTATGG CTTCACCCACTGTGGAGACACCAATGAGTGCAGCATCAACAACGGAGGCTGTCAGCAGGTCTGTGTGAAC ACAGTGGGCAGCTATGAATGCCAGTGCCACCCTGGGTACAAGCTCCACTGGAATAAAAAAGACTGTGTGG AAGTGAAGGGGCTCCTGCCCACAAGTGTGTCACCCCGTGTGTCCCTGCACTGCGGTAAGAGTGGTGGAGG AGACGGGTGCTTCCTCAGATGTCACTCTGGCATTCACCTCTCTTCAGATGTCACCACCATCAGGACAAGT GTAACCTTTAAGCTAAATGAAGGCAAGTGTAGTTTGAAAAATGCTGAGCTGTTTCCCGAGGGTCTGCGAC CAGCACTACCAGAGAAGCACAGCTCAGTAAAAGAGAGCTTCCGCTACGTAAACCTTACATGCAGCTCTGG CAAGCAAGTCCCAGGAGCCCCTGGCCGACCAAGCACCCCTAAGGAAATGTTTATCACTGTTGAGTTTGAG CTTGAAACTAACCAAAAGGAGGTGACAGCTTCTTGTGACCTGAGCTGCATCGTAAAGCGAACCGAGAAGC GGCTCCGTAAAGCCATCCGCACGCTCAGAAAGGCCGTCCACAGGGAGCAGTTTCACCTCCAGCTCTCAGG CATGAACCTCGACGTGGCTAAAAAGCCTCCCAGAACATCTGAACGCCAGGCAGAGTCCTGTGGAGTGGGC CAGGGTCATGCAGAAAACCAATGTGTCAGTTGCAGGGCTGGGACCTATTATGATGGAGCACGAGAACGCT GCATTTTATGTCCAAATGGAACCTTCCAAAATGAGGAAGGACAAATGACTTGTGAACCATGCCCAAGACC AGGAAATTCTGGGGCCCTGAAGACCCCAGAAGCTTGGAATATGTCTGAATGTGGAGGTCTGTGTCAACCT GGTGAATATTCTGCAGATGGCTTTGCACCTTGCCAGCTCTGTGCCCTGGGCACGTTCCAGCCTGAAGCTG GTCGAACTTCCTGCTTCCCCTGTGGAGGAGGCCTTGCCACCAAACATCAGGGAGCTACTTCCTTTCAGGA CTGTGAAACCAGAGTTCAATGTTCACCTGGACATTTCTACAACACCACCACTCACCGATGTATTCGTTGC CCAGTGGGAACATACCAGCCTGAATTTGGAAAAAATAATTGTGTTTCTTGCCCAGGAAATACTACGACTG ACTTTGATGGCTCCACAAACATAACCCAGTGTAAAAACAGAAGATGTGGAGGGGAGCTGGGAGATTTCAC TGGGTACATTGAATCCCCAAACTACCCAGGCAATTACCCAGCCAACACCGAGTGTACGTGGACCATCAAC CCACCCCCCAAGCGCCGCATCCTGATCGTGGTCCCTGAGATCTTCCTGCCCATAGAGGACGACTGTGGGG ACTATCTGGTGATGCGGAAAACCTCTTCATCCAATTCTGTGACAACATATGAAACCTGCCAGACCTACGA ACGCCCCATCGCCTTCACCTCCAGGTCAAAGAAGCTGTGGATTCAGTTCAAGTCCAATGAAGGGAACAGC GCTAGAGGGTTCCAGGTCCCATACGTGACATATGATGAGGACTACCAGGAACTCATTGAAGACATAGTTC GAGATGGCAGGCTCTATGCATCTGAGAACCATCAGGAAATACTTAAGGATAAGAAACTTATCAAGGCTCT GTTTGATGTCCTGGCCCATCCCCAGAACTATTTCAAGTACACAGCCCAGGAGTCCCGAGAGATGTTTCCA AGATCGTTCATCCGATTGCTACGTTCCAAAGTGTCCAGGTTTTTGAGACCTTACAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224136 representing NM_020974
Red=Cloning site Green=Tags(s) MGVAGRNRPGAAWAVLLLLLLLPPLLLLAGAVPPGRGRAAGPQEDVDECAQGLDDCHADALCQNTPTSYK CSCKPGYQGEGRQCEDIDECGNELNGGCVHDCLNIPGNYRCTCFDGFMLAHDGHNCLDVDECLENNGGCQ HTCVNVMGSYECCCKEGFFLSDNQHTCIHRSEEGLSCMNKDHGCSHICKEAPRGSVACECRPGFELAKNQ RDCILTCNHGNGGCQHSCDDTADGPECSCHPQYKMHTDGRSCLEREDTVLEVTESNTTSVVDGDKRVKRR LLMETCAVNNGGCDRTCKDTSTGVHCSCPVGFTLQLDGKTCKDIDECQTRNGGCDHFCKNIVGSFDCGCK KGFKLLTDEKSCQDVDECSLDRTCDHSCINHPGTFACACNRGYTLYGFTHCGDTNECSINNGGCQQVCVN TVGSYECQCHPGYKLHWNKKDCVEVKGLLPTSVSPRVSLHCGKSGGGDGCFLRCHSGIHLSSDVTTIRTS VTFKLNEGKCSLKNAELFPEGLRPALPEKHSSVKESFRYVNLTCSSGKQVPGAPGRPSTPKEMFITVEFE LETNQKEVTASCDLSCIVKRTEKRLRKAIRTLRKAVHREQFHLQLSGMNLDVAKKPPRTSERQAESCGVG QGHAENQCVSCRAGTYYDGARERCILCPNGTFQNEEGQMTCEPCPRPGNSGALKTPEAWNMSECGGLCQP GEYSADGFAPCQLCALGTFQPEAGRTSCFPCGGGLATKHQGATSFQDCETRVQCSPGHFYNTTTHRCIRC PVGTYQPEFGKNNCVSCPGNTTTDFDGSTNITQCKNRRCGGELGDFTGYIESPNYPGNYPANTECTWTIN PPPKRRILIVVPEIFLPIEDDCGDYLVMRKTSSSNSVTTYETCQTYERPIAFTSRSKKLWIQFKSNEGNS ARGFQVPYVTYDEDYQELIEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESREMFP RSFIRLLRSKVSRFLRPYK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020974 |
| ORF Size | 2997 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020974.1, NP_066025.1 |
| RefSeq Size | 3737 bp |
| RefSeq ORF | 2916 bp |
| Locus ID | 57758 |
| UniProt ID | Q9NQ36 |
| Domains | CUB, EGF_CA, EGF, EGF |
| Protein Families | Transmembrane |
| Gene Summary | Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed:22902404, PubMed:22677548). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224136 | SCUBE2 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 1 |
CNY 9,790.00 |
|
| RC224136L3 | Lenti-ORF clone of SCUBE2 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 1 |
CNY 11,690.00 |
|
| RC224136L4 | Lenti-ORF clone of SCUBE2 (mGFP-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 1 |
CNY 11,690.00 |
|
| SC113056 | SCUBE2 (untagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 1 |
CNY 7,320.00 |
