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Ist1 (NM_001017454) Rat Untagged Clone
CAT＃: RN201868
Ist1 (untagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1307799 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201868 representing NM_001017454
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCTCTGGTTTCAAAGCTGAGCGCTTACGAGTCAATTTGAGATTAGTTATAAATCGTCTTAAAC TGTTGGAGAAAAAAAAAACGGAACTGGCTCAAAAAGCGAGGAAGGAGATTGCTGACTATCTGGCCGCTGG GAAGGACGAGAGAGCACGTATCCGTGTGGAGCACATAATCCGGGAAGACTACCTTGTGGAGGCCATGGAG ATCCTGGAGCTGTACTGTGACCTGCTGCTGGCTCGGTTTGGCCTTATCCAGTCTATGAAGGAGCTAGATT CTGGTCTGGCTGAATCTGTGTCTACACTGATCTGGGCCGCTCCTCGGTTGCAGTCAGAAGTGGCTGAGTT GAAAATAGTTGCTGATCAGCTCTGTGCCAAATACAGCAAGGAATATGGCAAGTTGTGCAGGACCAACCAG ATCGGGACTGTGAATGATAGGCTAATGCATAAACTGAGTGTAGAAGCCCCACCCAAGATCCTAGTGGAGA GATACCTGATTGAAATTGCCAAGAATTACAATGTGCCCTATGAACCTGACTCTGTGGTCATGGCAGAAGC TCCCGTTGGAGTGGAGACAGATCTTATTGATGTTGGATTTACAGATGATGTGAAGAAAGGTGGTCCTGGA AGAGGAGGAGGGGGCGGGTTCACAGCCCCAGTTGGTGCACCTGATGGGACCATGCCCATGCCCATGCCCA TGCCCATGCCCATGCCATCGCCATCTCCAAATGCTCCCTTCGCATACCCACTGCCAAAGGGACCATCGGA TTTCAGTGGATTGCCAGTGGGGACTTACCAGGCCTTTCCTAACATTCACCCACCCCAGATACCAGCAACT CCCCCATCTTACGAATCTGTTGATGACATTAATGCTGATAAAAATGTTTCTTCGGCACAGATTGTTGGAC CCAAGCCAGAAGCTCCTGCAAAGCCCCCTTCCAGACCTGTGGATAACTACAACACCTTTGTACTGCCAGA GTTGCCATCGGTGCCAGACACACTCCCGACCGCATCTGCCGGAGCCAGTACCTCGGCGTCAGAGGACATA GACTTTGATGATCTTTCCCGGAGATTTGAGGAGCTGAAAAAGAAAACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001017454 |
| Insert Size | 1101 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001017454.2, NP_001017454.1 |
| RefSeq Size | 2261 bp |
| RefSeq ORF | 1101 bp |
| Locus ID | 307833 |
| UniProt ID | Q568Z6 |
| Gene Summary | ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201868 | Ist1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug) |
CNY 3,990.00 |
|
| RR201868L3 | Lenti ORF clone of Ist1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug) |
CNY 6,080.00 |
|
| RR201868L4 | Lenti ORF clone of Ist1 (mGFP-tagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug) |
CNY 6,650.00 |
