Ist1 (NM_001017454) Rat Tagged Lenti ORF Clone
CAT#: RR201868L3
- LentiORF®
Lenti ORF clone of Ist1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug)
"NM_001017454" in other vectors (3)
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CNY 6,080.00
CNY 600.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RGD1307799 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR201868).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001017454 |
ORF Size | 1098 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001017454.2 |
RefSeq Size | 2261 bp |
RefSeq ORF | 1101 bp |
Locus ID | 307833 |
UniProt ID | Q568Z6 |
Gene Summary | ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN201868 | Ist1 (untagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug) |
CNY 3,990.00 |
|
RR201868 | Ist1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug) |
CNY 3,990.00 |
|
RR201868L4 | Lenti ORF clone of Ist1 (mGFP-tagged ORF) - Rat similar to RIKEN cDNA 2400003C14 (RGD1307799), (10 ug) |
CNY 6,650.00 |