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PPP1R15B (NM_032833) Human Untagged Clone
CAT＃: SC103080
PPP1R15B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15B (PPP1R15B)
CNY 5,232.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CREP; MSSGM2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC103080 sequence for NM_032833 edited (data generated by NextGen Sequencing)
ATGGAGCCGGGGACAGGCGGATCGCGGAAACGGCTTGGCCCTCGGGCGGGCTTCCGGTTC TGGCCACCCTTTTTCCCTCGGCGATCGCAAGCAGGCTCTTCTAAGTTCCCGACGCCTCTT GGCCCGGAAAACTCCGGGAACCCCACACTGCTTTCCTCTGCCCAGCCCGAGACTCGGGTC AGTTACTGGACGAAACTGCTCTCCCAGCTCCTTGCGCCGCTCCCCGGATTGCTTCAGAAG GTGCTAATTTGGAGCCAACTTTTCGGTGGAATGTTTCCGACCAGATGGCTAGATTTTGCT GGAGTCTACAGCGCCCTGAGAGCCCTGAAGGGACGGGAGAAACCAGCCGCCCCCACAGCG CAGAAATCTTTGAGTTCGCTGCAGCTCGACTCCTCAGACCCCTCGGTCACCAGTCCCCTT GATTGGCTAGAGGAGGGGATCCACTGGCAATACTCGCCCCCAGACCTAAAATTGGAGCTT AAGGCCAAGGGAAGTGCTTTGGACCCTGCAGCACAGGCTTTTCTCTTAGAGCAGCAGCTG TGGGGAGTGGAGCTGTTGCCCAGTAGCCTTCAATCCCGTCTGTACTCTAACCGGGAACTT GGCTCTTCGCCCTCTGGGCCTCTAAACATTCAACGCATAGACAATTTCAGTGTGGTATCC TATTTGCTGAACCCTTCCTACCTGGACTGCTTTCCTAGGCTAGAAGTCAGCTATCAGAAC AGTGATGGAAATAGCGAGGTAGTCGGCTTCCAGACACTAACCCCAGAGAGCAGCTGCCTG AGAGAGGACCATTGTCATCCCCAGCCGCTGAGTGCAGAACTCATTCCGGCCTCGTGGCAG GGATGTCCACCTCTTTCTACGGAAGGCCTACCAGAAATTCACCATCTTCGCATGAAACGG CTGGAATTCCTTCAACAGGCTAGCAAGGGGCAAGATTTACCCACCCCTGACCAGGATAAT GGCTACCACAGCCTGGAGGAGGAACACAGCCTTCTCCGGATGGATCCAAAACACTGCAGA GATAACCCAACACAGTTTGTTCCTGCTGCTGGAGACATTCCTGGAAACACCCAGGAATCC ACTGAAGAAAAAATAGAATTATTAACTACAGAGGTTCCACTTGCTTTGGAAGAAGAGAGC CCTTCTGAGGGCTGTCCATCTAGTGAGATACCTATGGAAAAGGAGCCTGGAGAGGGCCGA ATAAGTGTAGTTGATTACTCATACCTAGAAGGTGACCTTCCCATTTCTGCCAGACCAGCT TGTAGTAACAAACTGATAGATTATATTTTGGGAGGTGCATCCAGTGACCTGGAAACAAGT TCTGATCCAGAAGGTGAGGATTGGGATGAGGAAGCTGAGGATGATGGTTTTGATAGTGAT AGCTCACTGTCAGACTCAGACCTTGAACAAGACCCTGAAGGGCTTCACCTTTGGAACTCT TTCTGCAGTGTAGATCCTTATAATCCCCAGAACTTTACAGCAACAATTCAGACTGCTGCC AGAATTGTTCCTGAAGAGCCTTCTGATTCAGAGAAGGATTTGTCTGGCAAGTCTGATCTA GAGAATTCCTCCCAGTCTGGAAGCCTTCCTGAGACCCCTGAGCATAGTTCTGGGGAGGAA GATGACTGGGAATCTAGTGCAGATGAAGCAGAGAGTCTCAAACTGTGGAACTCATTCTGT AATTCTGATGACCCCTACAACCCTTTAAATTTTAAGGCTCCTTTTCAAACATCAGGGGAA AATGAGAAAGGCTGTCGTGACTCAAAGACCCCATCTGAGTCCATTGTGGCCATTTCTGAG TGTCACACCTTACTTTCTTGTAAGGTGCAGCTGTTGGGGAGCCAAGAAAGTGAATGTCCA GACTCGGTACAGCGTGACGTTCTTTCTGGAGGAAGACACACACATGTCAAAAGAAAAAAG GTAACCTTCCTTGAAGAAGTTACTGAGTATTATATAAGTGGTGATGAGGATCGCAAAGGA CCATGGGAAGAATTTGCAAGGGATGGATGCAGGTTCCAGAAACGAATTCAAGAAACAGAA GATGCTATTGGATATTGCTTGACATTTGAACACAGAGAAAGAATGTTTAATAGACTCCAG GGAACATGCTTCAAAGGACTTAATGTTCTCAAGCAATGTTGA Clone variation with respect to NM_032833.3 923 a=>g >OriGene 5' read for NM_032833 unedited
GGGCCCGGGGGCNNCNNCCCCNCTTTTCCCCCCCCCCCCGTTCAAATTTGTNATACGACT CACTATAGGCGGCCGCGNAATTCGCACGAGNACCGCACCAGCCGGCCTACCCAGTCCTTC CGGTATCGCGTTGCTCAGGGGCTTTTCAACCCTTCTGTCAGTCGGAAAACCATCGCCGAG GCCGTGGGGGGACTCCTATCCATGGTGTTGAAGCGTCGAGCCGACTAGGGAACCTCCTTC CCCGCCAGGATGGAAGTCGCATCAGTCGCCGCCTATTGCGCGGGCTGTTCTTCCCTGTGT TCTGCCGCCCGCTGCCGCATTCGCTGCCCTCTGTGGCTTTTCTGCTGGCTCGAAGATCGG CCTGGAGCAGCGACGCCACCGCTGGGCAAGGCCGAGACTCTGTAGGCTTCCTCCGAATCC CGTCGACCTCCAGCCGCTGAGCGCCGCGGCCCTACCTGAGAGACTGTCAAGAAAAAGGAG ATGGAGCCGGGGACAGGCGGATCGCGGAAACGGCTTGGCCCTCGGGCGGGCTTCCGGTTC TGGCCACCCTTTTTCCCTCGGCGATCGCAAGCAGGCTCTTCTAAGTTCCCGACGCCTCTT GGCCCGGAAAACTCCGGGAACCCCACACTGCTTTCCTCTGCCCAGCCCGAGACTCGGGTC AGTTACTGGACGAAACTGCTCTCCCAGCTCCTTGCGCCGCTCCCCGGATTGCTTCAGAAA GTGCTAATTTGGAGCCAACTTTTCGGTGGAATGTTTCCGACCAGATGGCTAGATTTTGCT GGAGTCTACAGCGCCCCTGAGAGCCTGAAGGGACCGGAGCAAACACCCGGCCCCACAGNG CAGAAATCTTTGGATTCGCTGCAGCTCGACTCCCCAAAACCCTCGGGCACCAACCCCCTT GAATGGCTAGAGGAGGGGATCCCACTGGCATCACCCCCCCCCAAACCCTAAATGGAGCCT AAGCCCAGGGA >OriGene 3' read for NM_032833 unedited
NAAAATTTTTTCTATGGACCGGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTT TGATATTGACTGAAGTTTATATTTTACATTTCAAACGTGAAAATTCAGAACTGCCCATTT CCAATTTTACAGTGGGATCCTGACAGGTTTTAAAAGTGACAGTGCTGAGGCATGATATTC ATTAACACTGGTTTTCTGTTGAGAACATATACACCAAAACCAAATTGCTCTGAGATGTCT CCTATTTTCTTTCTAGGAAATTTCTAGGCTTTTAAGTTTAAAAAATAAATGATTATGATG TAATTATTACTTTCCAGAGTGTTTTGCAATAACTTCAACCTGTTAAGAGATACAAAGAAC TATATTAAACTGGGAACTACAATAACGTACACAGAACCCTCTTCAAAGAAATTAAATATA TTAGATGTTAAAATGTGGTAGAAAGATGCAGCTTTCCCAAAGTAGTAAAGTACTGCACAT ATGGGTTTTGTGGCAGTCCTTGGAAATATCCTAGGTAGAACTTAATGTAGAAATAAAAAG GCTACCACATATTTTCAATCCAAGTCATTTTTACAAGAAAAAAAAAAGTGACACAAAATA ATGCACTTTAAGTTGGTAGCATACACAAGGTTATTTTTTAGCCTAACATAGACAGGCCAA ATCATTGAAATAAAAAAAATATAGAAAAACATAAAAGCCCATTAACTTCTGAATTTTGGG AAAGAAACAAGAAAGAGCCCAAAGTTTTCAGATAGGCACACATAATTTAGATTAGAAATG AAAATGGGCTTTAAGCCCTATAAATATTGTTTTTCCAAGAAAATAAGTTCTGAAAGTGCA AAATGACAACTCAAAAAGGTCCCCCTTTCCACCTCATGTCAGCACAANACCTTTAAAAGC ACCATCCACTAATCCAAAAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032833 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032833.2, NP_116222.2 |
| RefSeq Size | 5264 bp |
| RefSeq ORF | 2142 bp |
| Locus ID | 84919 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism. [provided by RefSeq, Feb 2016] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209489 | PPP1R15B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15B (PPP1R15B) |
CNY 5,224.00 |
|
| RC209489L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 15B (PPP1R15B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209489L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 15B (PPP1R15B), mGFP tagged |
CNY 5,890.00 |
|
| RG209489 | PPP1R15B (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 15B (PPP1R15B) |
CNY 4,370.00 |
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