IRE1 (ERN2) (NM_033266) Human Untagged Clone

CAT#: SC323535

ERN2 (untagged)-Kinase deficient mutant (K596M) of Human endoplasmic reticulum to nucleus signaling 2 (ERN2)



  "NM_033266" in other vectors (7)

CNY 7,144.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-ERN2 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms hIRE2p; IRE1-BETA; IRE1b; IRE2p
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_033266, the custom clone sequence may differ by one or more nucleotides


ATGAGAAGGGCGGGGATCGGCGAGGACTCCAGGCTGGGGTTGCAGGCCCAGCCAGGGGCGGAGCCTTCTC
CGGGTCGGGCGGGGACAGAGCGCTCCCTTGGAGGCACCCAGGGACCTGGCCAGCCGTGCAGCTGCCCAGG
CGCTATGGCGAGTGCGGTCAGGGGGTCGAGGCCGTGGCCCCGGCTGGGGCTCCAGCTCCAGTTCGCGGCG
CTGCTGCTCGGGACGCTGAGTCCACAGGTTCATACTCTCAGGCCAGAGAACCTCCTGCTGGTGTCCACCT
TGGATGGAAGTCTCCACGCACTAAGCAAGCAGACAGGGGACCTGAAGTGGACTCTGAGGGATGATCCCGT
CATCGAAGGACCAATGTACGTCACAGAAATGGCCTTTCTCTCTGACCCAGCAGATGGCAGCCTGTACATC
TTGGGGACCCAAAAACAACAGGGATTAATGAAACTGCCATTCACCATCCCTGAGCTGGTTCATGCCTCTC
CCTGCCGCAGCTCTGATGGGGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTGGTGGACCCTGA
GTCAGGGGAGACCCAGATGACACTGACCACAGAGGGTCCCTCCACCCCCCGCCTCTACATTGGCCGAACA
CAGTATACGGTCACCATGCATGACCCAAGAGCCCCAGCCCTGCGCTGGAACACCACCTACCGCCGCTACT
CAGCGCCCCCCATGGATGGCTCACCTGGGAAATACATGAGCCACCTGGCGTCCTGCGGGATGGGCCTGCT
GCTCACTGTGGACCCAGGAAGCGGGACGGTGCTGTGGACACAGGACCTGGGCGTGCCTGTGATGGGCGTC
TACACCTGGCACCAGGACGGCCTGCGCCAGCTGCCGCATCTCACGCTGGCTCGAGACACTCTGCATTTCC
TCGCCCTCCGCTGGGGCCACATCCGACTGCCTGCCTCAGGCCCCCGGGACACAGCCACCCTCTTCTCTAC
CTTGGACACCCAGCTGCTAATGACGCTGTATGTGGGGAAGGATGAAACTGGCTTCTATGTCTCTAAAGCA
CTGGTCCACACAGGAGTGGCCCTGGTGCCTCGTGGACTGACCCTGGCCCCCGCAGATGGCCCCACCACAG
ATGAGGTGACACTCCAAGTCTCAGGAGAGCGAGAGGGCTCACCCAGCACTGCTGTTAGATACCCCTCAGG
CAGTGTGGCCCTCCCAAGCCAGTGGCTGCTCATTGGACACCACGAGCTACCCCCAGTCCTGCACACCACC
ATGCTGAGGGTCCATCCCACCCTGGGGAGTGGAACTGCAGAGACAAGACCTCCAGAGAATACCCAGGCCC
CAGCCTTCTTCTTGGAGCTATTGAGCCTGAGCCGAGAGAAACTTTGGGACTCCGAGCTGCATCCAGAAGA
AAAAACTCCAGACTCTTACTTGGGGCTGGGACCCCAAGACCTGCTGGCAGCTAGCCTCACTGCTGTCCTC
CTGGGAGGGTGGATTCTCTTTGTGATGAGGCAGCAACAGCCGCAGGTGGTGGAGAAGCAGCAGGAGACCC
CCCTGGCACCTGCAGACTTTGCTCACATCTCCCAGGATGCCCAGTCCCTGCACTCGGGGGCCAGCCGGAG
GAGCCAGAAGAGGCTTCAGAGTCCCTCAAAGCAAGCCCAGCCACTCGACGACCCTGAAGCTGAGCAACTC
ACCGTAGTGGGGAAGATTTCCTTCAATCCCAAGGACGTGCTGGGCCGCGGGGCAGGCGGGACTTTCGTTT
TCCGGGGACAGTTTGAGGGACGGGCAGTGGCTGTCAAGCGGCTCCTCCGCGAGTGCTTTGGCCTGGTTCG
GCGGGAAGTTCAACTGCTGCAGGAGTCTGACAGGCACCCCAACGTGCTCCGCTACTTCTGCACCGAGCGG
GGACCCCAGTTCCACTACATTGCCCTGGAGCTCTGCCGGGCCTCCTTGCAGGAGTACGTAGAAAACCCGG
ACCTGGATCGCGGGGGTCTGGAGCCCGAGGTCGTGCTGCAGCAGCTGATGTCTGGCCTGGCCCACCTGCA
CTCTTTACACATAGTGCACCGGGACCTGAAGCCAGGAAATATTCTCATCACCGGGCCTGACAGCCAGGGC
CTGGGCAGAGTGGTGCTCTCAGACTTCGGCCTCTGCAAGAAGCTGCCTGCTGGCCGCTGTAGCTTCAGCC
TCCACTCCGGCATCCCCGGCACGGAAGGCTGGATGGCGCCCGAGCTTCTGCAGCTCCTGCCACCAGACAG
TCCTACCAGCGCTGTGGACATCTTCTCTGCAGGCTGCGTGTTCTACTACGTGCTTTCTGGTGGCAGCCAC
CCCTTTGGAGACAGTCTTTATCGCCAGGCAAACATCCTCACAGGGGCTCCCTGTCTGGCTCACCTGGAGG
AAGAGGTCCACGACAAGGTGGTTGCCCGGGACCTGGTTGGAGCCATGTTGAGCCCACTGCCGCAGCCACG
CCCCTCTGCCCCCCAGGTGCTGGCCCACCCCTTCTTTTGGAGCAGAGCCAAGCAACTCCAGTTCTTCCAG
GACGTCAGTGACTGGCTGGAGAAGGAGTCCGAGCAGGAGCCCCTGGTGAGGGCACTGGAGGCGGGAGGCT
GCGCAGTGGTCCGGGACAACTGGCACGAGCACATCTCCATGCCGCTGCAGACAGATCTGAGAAAGTTCCG
GTCCTATAAGGGGACATCAGTGCGAGACCTGCTCCGTGCTGTGAGGAACAAGAAGCACCACTACAGGGAG
CTCCCAGTTGAGGTGCGACAGGCACTCGGCCAAGTCCCTGATGGCTTCGTCCAGTACTTCACAAACCGCT
TCCCACGGCTGCTCCTCCACACGCACCGAGCCATGAGGAGCTGCGCCTCTGAGAGCCTCTTCCTGCCCTA
CTACCCGCCAGACTCAGAGGCCAGGAGGCCATGCCCTGGGGCCACAGGGAGGTGA


>OriGene 5' read for mutant NM_033266 unedited
ACGCCCTTCCCGCAACGGGCGGAGGCGTAACGGAGGGAGGACACATAAGCAGAGCACGAACAGCGAACCG
TCAGAACAAAGTAATACGACACACAATAGGGCGGCCGCGAAAACGGCACCAGCATCGAAGGACCAATGTA
CGACACAGAAACGGCCTTTCTCTCTGACCCAGCAGATGGCAGCCTGTACATCTTGGGGACCCAAAAACAA
CAGGGATTAATGAAACTGCCATTTCACCATCCCTGAGCTGGTTTCATGCCTCTCCCTGCCGCAGCTCTGA
TGGGGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTTGTGGTGGACCCTGAGTCAGGGGAGACCCA
GATGACACTGACCACAGAAGGGTCCCCTCCAACCCCCCCGCCTCTACATTGCCCGAACCACAGTATACGG
TCACCATGGCATGAACCCAAGAGCCCCCAGCCCTGCCGCTGGAACCACCACCTACCCGCGCTACTCAGCC
GCCCCCCCATGGGATGCCTAACCTGGGGAAAATCCATGGACCCACCTGGCCGTCCCTGCGGGATGGGGCC
TGGCTGCTTAACGTTGGACCCCAGAAGCGGGACGTTCCTTTGAACAAGGACCCTGGCGTTGCCTGTGAAT
GGCCTTTCACCCTGGCACCCAGACGGGCTGGCCAGCTTCGCGATTTTAAGCTGGGTCGGACACTCTGCAA
TTCCGCCTCTGCTGTGGGCCATTCGAATGCCTGCGCTAGGCCCCGGAACAACACCCTTTCTACTTGGACA
CCACTGCTATAGACCTTAGTGGAGGATAACCGGTCTATGTTTAAGACTGGTCACGATGCCTGTCCTTGAT
GACTGCCCGATGCCACAGATGTACTCAGTCAGACCAAGTCAACGACTGGTATCTACGTGCTCACAT
>SC323535 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TCATGAGTGCTGGGCCGCGGGGCAGGCGGGACTTTCGTTTTCCGGGGACAGTTTGAGGGACGGGCAGTGG
CTGTCATGCGGCTCCTCCGCGAGTGCTTTGGCCTGGTTCGGCGGGAAGTTCAACTGCTGCAGGAGTCTGA
CAGGCACCCCAACGTGCTCCGCTACTTCTGCACCGAGCGGGGACCCCAGTTCCACTACATTGCCCTGGAG
CTCTGCCGGGCCTCCTTGCAGGAGTACGTAGAAAACCCGGACCTG
Restriction Sites Please inquire     
ACCN NM_033266
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_033266.2, NP_150296.2
RefSeq Size 3549 bp
RefSeq ORF 2925 bp
Locus ID 10595
UniProt ID Q76MJ5
Protein Families Druggable Genome, Protein Kinase
Gene Summary Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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