hexon (AC_000018) Virus Tagged ORF Clone

CAT#: VC100192

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 7], codon optimized for human cell expression, AP_000548



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CNY 14,060.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100192 represents NCBI reference of AP_000548 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTCCCTCTATGATGCCCCAATGGGCATACATGCATATCGCAGGACAGGACGCCAGCGAATATC
TGTCACCCGGGCTCGTGCAGTTTGCTCGCGCTACCGACACCTACTTTTCAATGGGGAATAAGTTCAGGAA
CCCCACCGTGGCACCAACTCACGATGTGACAACTGACAGATCTCAGAGATTGATGCTCCGGTTCGTGCCT
GTTGATAGAGAGGACAATACCTATAGTTACAAGGTTAGGTATACACTGGCTGTGGGCGATAACAGGGTGC
TGGACATGGCCAGTACCTTTTTCGATATAAGAGGTGTGCTCGATCGAGGACCAAGCTTTAAGCCCTATAG
TGGGACTGCTTATAACAGCCTGGCGCCAAAGGGCGCTCCAAACACCAGCCAGTGGATAGTCACAACCGGG
GAGGATAACGCTACAACCTATACATTCGGCATTGCCTCCACCAAAGGCGACAACATAACAAAGGAAGGCC
TTGAAATCGGAAAGGACATAACCGCCGATAACAAGCCCATCTACGCAGATAAGACCTACCAGCCTGAGCC
GCAGGTCGGGGAGGAATCCTGGACAGACATTGACGGGACGAATGAAAAGTTTGGGGGACGAGCCTTGAAG
CCAGCCACTAAAATGAAGCCGTGTTACGGGTCCTTTGCCCGCCCTACTAACATCAAAGGGGGCCAGGCGA
AGAACAGGAAGGTGACTCCTACGGAGGGAGATGTTGAGGCAGAGGAACCAGACATCGATATGGAATTCTT
TGATGGTAGGGAAGCGGCCGATGCTTTCTCACCAGAGATTGTATTGTATACCGAGAATGTCAACCTGGAG
ACACCGGACAGCCACGTAGTTTACAAACCCGGAACCAGTGACGGAAATAGTCATGCTAACCTCGGTCAGC
AAGCGATGCCCAATAGGCCCAATTATATTGGGTTTCGGGACAACTTTGTCGGGCTCATGTACTATAACTC
TACCGGAAATATGGGTGTACTCGCAGGACAGGCTAGTCAGCTGAACGCTGTAGTAGACCTGCAGGACCGC
AACACGGAATTGAGCTATCAGCTCTTGTTGGACAGTCTCGGAGACCGCAGCCGATATTTTAGCATGTGGA
ATCAAGCAGTGGACAGCTATGATCCGGACGTGAGGATTATTGAAAATCACGGTGTTGAAGATGAGCTGCC
CAACTACTGCTTTCCGCTGGACGGAATCGGACCCGGGAACAAGTACCAAGGTATTAAACCTCGCGATACT
GCTTGGGAGAAGGACACCAAAGTATCAACTGCAAATGAGATAGCTATTGGAAACAACCTCGCCATGGAAA
TCAATATACAGGCCAACCTTTGGCGCTCATTTCTTTATTCCAACGTTGCCCTGTACCTGCCAGATGTCTA
CAAGTACACACCAACTAATATCACACTGCCAGCAAATACAAACACTTACGAGTACATGAATGGAAGAGTC
GTCTCCCCCAGTCTGGTGGACTCATACATCAACATTGGTGCACGGTGGTCCTTGGATCCGATGGACAACG
TGAATCCTTTTAATCATCACAGGAACGCCGGTCTTAGGTATAGGTCAATGCTCCTTGGGAACGGCAGATA
TGTGCCATTCCATATCCAGGTCCCTCAGAAATTCTTTGCAGTAAAGAACCTTCTTCTTCTCCCGGGCAGC
TACACCTATGAATGGAACTTTCGCAAGGATGTCAATATGGTTTTGCAGTCTTCACTTGGCAATGATCTGC
GGACCGATGGGGCAAATATCAGTTTCACTTCCATAAACCTGTACGCAACGTTTTTCCCCATGGCACACAA
CACGGCATCTACACTTGAGGCCATGTTGAGAAACGACACCAATGACCAGTCATTCAATGACTATCTCTCT
GCGGCGAATATGCTCTACCCAATTCCAGCGAATGCTACTAATATACCCATCTCTATCCCAAGTCGGAATT
GGGCTGCCTTTAGGGGATGGTCTTTTACACGACTCAAAACAAAGGAGACGCCCAGCCTGGGGAGCGGCTT
CGATCCTTATTTTGTTTATAGCGGGAGTATTCCATACCTGGACGGTACATTTTACCTGAACCACACATTC
AAGAAAGTTTCTATTATGTTCGACTCCTCAGTGAGCTGGCCCGGCAACGATCGACTGCTGTCTCCCAATG
AATTCGAAATCAAGCGGACAGTGGACGGGGAGGGCTATAATGTAGCTCAGTGTAATATGACCAAAGACTG
GTTCCTGGTCCAGATGCTGGCAAATTACAATATCGGGTACCAAGGTTTTTATATTCCGGAGGGATACAAA
GATAGGATGTACTCATTCTTTAGGAATTTTCAACCAATGTCTCGGCAGGTGGTCGACGAGGTAAACTACA
CCGACTATAAGGCAGTCACTCTGCCCTACCAGCATAACAACAGCGGATTCGTTGGATACCTCGCTCCCAC
GATGCGCCAGGGTGAGCCGTACCCCGCTAATTACCCCTACCCTCTTATCGGGACCACGGCAGTGAAAAGC
GTGACACAGAAGAAGTTCCTCTGCGACCGCACCATGTGGCGGATTCCCTTCAGTTCTAACTTCATGTCAA
TGGGTGCCTTGACTGACCTCGGCCAGAACTTGCTGTACGCTAATTCCGCGCATGCCCTGGATATGACCTT
CGAAGTCGACCCTATGGATGAACCGACTCTGCTGTATCTGCTGTTCGAAGTGTTCGATGTAGTCCGCGTC
CATCAACCTCACAGAGGGGTGATCGAGGCAGTCTACCTCAGAACACCATTTTCCGCTGGCAACGCAACCA
CC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100192 representing AP_000548
Red=Cloning sites Green=Tags

MATPSMMPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSMGNKFRNPTVAPTHDVTTDRSQRLMLRFVP
VDREDNTYSYKVRYTLAVGDNRVLDMASTFFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNTSQWIVTTG
EDNATTYTFGIASTKGDNITKEGLEIGKDITADNKPIYADKTYQPEPQVGEESWTDIDGTNEKFGGRALK
PATKMKPCYGSFARPTNIKGGQAKNRKVTPTEGDVEAEEPDIDMEFFDGREAADAFSPEIVLYTENVNLE
TPDSHVVYKPGTSDGNSHANLGQQAMPNRPNYIGFRDNFVGLMYYNSTGNMGVLAGQASQLNAVVDLQDR
NTELSYQLLLDSLGDRSRYFSMWNQAVDSYDPDVRIIENHGVEDELPNYCFPLDGIGPGNKYQGIKPRDT
AWEKDTKVSTANEIAIGNNLAMEINIQANLWRSFLYSNVALYLPDVYKYTPTNITLPANTNTYEYMNGRV
VSPSLVDSYINIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAVKNLLLLPGS
YTYEWNFRKDVNMVLQSSLGNDLRTDGANISFTSINLYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLS
AANMLYPIPANATNIPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGSIPYLDGTFYLNHTF
KKVSIMFDSSVSWPGNDRLLSPNEFEIKRTVDGEGYNVAQCNMTKDWFLVQMLANYNIGYQGFYIPEGYK
DRMYSFFRNFQPMSRQVVDEVNYTDYKAVTLPYQHNNSGFVGYLAPTMRQGEPYPANYPYPLIGTTAVKS
VTQKKFLCDRTMWRIPFSSNFMSMGALTDLGQNLLYANSAHALDMTFEVDPMDEPTLLYLLFEVFDVVRV
HQPHRGVIEAVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000018
ORF Size 2802 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000018.1, AP_000548
RefSeq ORF 2802 bp
MW 105.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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