pTP (AC_000018) Virus Tagged ORF Clone

CAT#: VC100215

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 7], codon optimized for human cell expression, AP_000540



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CNY 7,890.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100215 represents NCBI reference of AP_000540 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTGTCTATCAACGACTGTGCGAGGCTGACCGGTCAAACCGTGCCTACAATGAATTACTTCATGC
CTCTGCGAAATATATGGAATCGGGTAAGGGAGTTTCCGAGAGCTAGCACTACAGCCTCAGGTATTACATG
GATGTCCCGGTATATTTACGGCTATCATAGATTGATGCTCGAAGATCTTGCGCCGGGAGCTCCCGCCACA
GAGCGCTGGCCACTGTACCGCCAGCCACCGCCCCACTTCCTTATTGGCTACCAGTACTTGGTGAGGACAT
GCAATGACTACATTTTCGATACCCGAGCTTATTCACGGCTGAAGTACACAGAACTCGTGCGCCCCGGGCA
CCAGACCGTGAACTGGAGCGTCATGGCCAATTGTAGTTATACAATCAATACAGGGGCTTATCATAGATTT
GTTGACTTCGATGATTTTCAGGCTACACTCACCCAGGTGCAACAAGCTATCTTGGCAGAGAGAGTTGTAG
CCGATCTCGCACTGGTGCAGCCGATGAGAGGCTTCGGAATTACCCGGATGCATGGGCGGGCGGGCGAGGA
GGAGGTCCCCGTGGAGCGCCTGATGCAAGATTACTACAAAGACCTGGCACGGTGTCAGGACAACGCATGG
GGAATGGCTCACAGACTCCGAATTCAGCAGGCAGGTCCGAAGGACCTGGTGCTTCTCGCCACAATCCGGA
GACTCAAGACTGCTTATTTTAATTTCATCACTTCTTCTATTGTTTCCAGTTCTCAGGAGGAGGAGGGTAA
AGAGAGGGAGAATCCTGATCGGGCAAGCAGTAGACCAAGGCCGCAGGAAACTGTGTTGTCCCTCCCTTGC
GATTGCGATTGGTTGGACGCCTTCGTGGAGAGATTTTCAGATCCTGTAGACTTGGAAACCATCAGGTCAC
TGCGCGGGGTGCCAACTGGTCAGCTCATCAAATGCATTATCAGCGCCGTATCCCTGCCTAATGGCGAGCC
ACCTAGTCACCATTTTCGCGAGATGAGGGGAGGGGTTTTCACGTTGCGGCCCCGGGAAAACGGGAGAGCG
GTCACCGAGACTATGAGGAGAAGGCGGGGAGAAGTGATCGAGAGGTTCATCGACAGATTGCCAGTGAGAC
GCCGGAGACGCAGGGTTCCCCCCCCACCAGCTGCACCCCCAGAAGAGGAGGAGATGCTTGTCGAGGAAGA
GGAAATCGAAGAAGAGATACCAGGGGACTTCGAACGGGAAGTAAGGACAACCATCGCTGAACTCATTAGG
CTCCTGGAGGAGGAGTTGACTGTGTCAGCCCGCAATTCTCAGTTCTTCAACTTCGCTGTAGATTTCTACG
AAGCAATGGAGCGGCTCGAGGCGCTGGGAGACGTATCCGAGATGCCCCTTCGGAGATGGATCATGTATTT
TTTTGTTACAGAGCACATCGCTACCACCTTGAACTACCTGTTTCAACGCCTCTGTAATTACGCTGTCTTT
ACTCGCCACGTGGAGCTGAACCTGGCCCAGGTTGTAATGAGGGCTCGCGATCCTGTTGGCGCTGTGGTGT
ACTCTCGAGTTTGGAACGAGGCCGGAATGAATGCTTTTTCCCAGTTGATAGGGCGCATTTCAAATGACCT
GGCGGCTACTGTTGAGCGGGCCGGCAGGGGTGACCTCCAGGAGGAAGAGATCCAGCAATTCATGGCAGAG
ATTGCGTACCAGGATAACTCTGGAGATGTTCAGGAGATTCTGCGACAGGCTGCCGTTAACGATACTGAGA
TCGATTCAGTGGAGCTGTCTTTTCGATTCAAACTCACCGGACCAGTCGCTTTTACCCAACGGAGGCAGAT
TCAGGACGTTAACAGGCGAGTAGTTGCTCACGCTTCCCTGCTGAGGGCTCAGTACCAGAATCTTCCCGCC
CGCGGGGCTGATGTTCCCCTTCCTGCCATGCCTCCAGGTCCTGAACCTCCCCTGCCTCCGGGGGCTAGAC
CCAGGCACCGCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100215 representing AP_000540
Red=Cloning sites Green=Tags

MALSINDCARLTGQTVPTMNYFMPLRNIWNRVREFPRASTTASGITWMSRYIYGYHRLMLEDLAPGAPAT
ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDTRAYSRLKYTELVRPGHQTVNWSVMANCSYTINTGAYHRF
VDFDDFQATLTQVQQAILAERVVADLALVQPMRGFGITRMHGRAGEEEVPVERLMQDYYKDLARCQDNAW
GMAHRLRIQQAGPKDLVLLATIRRLKTAYFNFITSSIVSSSQEEEGKERENPDRASSRPRPQETVLSLPC
DCDWLDAFVERFSDPVDLETIRSLRGVPTGQLIKCIISAVSLPNGEPPSHHFREMRGGVFTLRPRENGRA
VTETMRRRRGEVIERFIDRLPVRRRRRRVPPPPAAPPEEEEMLVEEEEIEEEIPGDFEREVRTTIAELIR
LLEEELTVSARNSQFFNFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVTEHIATTLNYLFQRLCNYAVF
TRHVELNLAQVVMRARDPVGAVVYSRVWNEAGMNAFSQLIGRISNDLAATVERAGRGDLQEEEIQQFMAE
IAYQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPA
RGADVPLPAMPPGPEPPLPPGARPRHRS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000018
ORF Size 1974 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000018.1, AP_000540
RefSeq ORF 1974 bp
MW 75.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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