100K (AC_000018) Virus Tagged ORF Clone
CAT#: VC100195
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 7], codon optimized for human cell expression, AP_000551
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CNY 12,540.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100195 represents NCBI reference of AP_000551 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACCCAACCATCCTTGCCCACAAGCCTGCAGGCGCCTTCACATCTGGCCCCATCATCCGATGAGG AAGAGTCACTGACCACACCGCCGCCTAGCCCTGCGACAACCACGAGTACGCTGGACGACGAGGAGGAGGA GGTTGACGCCGCACAGGAGATGCAGGCTCAGGATAATGTAAAGGCAGAGGAGATAGAGGCTGATGTGGAG CAGGATCCCGGATACGTGACACCTGCTGAACATGAAGAAGAATTGAAGCGCTTTATCGATCGCGAAGACG ATGACAGGCCCGAGCACCAGGCTGACGGCGACCACCAGGAGGCTGGGATTGGCGACCAAGTGGCAGACTA CCTTACGGGGCTGGGGGGCGAGGACGTTCTCCTGAAGCACCTTGCCCGCCAGTCTAATATCGTCAAGGAC GCCCTGCTCGATTTGACGGAAGTACCTATATCCGTGGAAGAACTTTCCAGAGCTTACGAATTGAACCTCT TCTCCCCCCACATCCCGCCAAAGCGCCAGCCCAATGGTACTTGCGAAGCTAACCCCCGGCTGAATTTTTA TCCCGCATTCACCGTGCCAGAAGTCCTGGCGACCTATCACATCTTTTTTAAAAACCAGAAAATACCCGTC AGCTGTAGGGCCAATCGGACACGCGCCGACGCCCTCCTTAATCTCGGACCCGGTGCGAGACTGCCCGACA TCGCTTCCCTCGAAGAAGTCCCTAAGATCTTCGAGGGGCTCGGCTCCGATGAGACCCGGGCAGCCAATGC TCTGCAGCAAGGGGAAAATGGAATGGATGAGCACCACTCAGCCCTCGTCGAACTCGAAGGTGATAACGCA AGACTCGCGGTCCTGAAGCGGTCTATAGTCGTGACCCATTTCGCTTATCCCGCTGTGAATCTGCCACCTA AGGTGATGTCTGCCGTAATGGACCACCTTTTGATAAAGCGAGCAGGCCCACTGAGCGAAAACCAAAACAT GCAGGACCCAGATGCTTCAGACGAGGGAAAACCCGTCGTCAGCGATGAACAACTGTCTCGATGGCTCGGC ACTAACAGCCCCCGGGACCTGGAGGAGCGACGCAAGCTGATGATGGCCGTTGTGCTGGTGACTGTAGAAT TGGAATGTCTGCGCCGCTTCTTCACTGACCCTGAAACCCTGCGGAAGCTGGAGGAGAACCTTCATTACAC ATTTAGACACGGCTTCGTGCGCCAGGCATGCAAAATCAGCAACGTAGAACTGACCAACTTGGTCAGCTAC ATGGGGATCCTGCACGAGAATAGACTGGGACAGAGTGTTCTGCACACCACCCTCAAGGGAGAAGCGCGCA GGGATTACATTAGGGACTGTGTGTACCTCTACCTGTGCCATACCTGGCAAACCGGGATGGGAGTATGGCA GCAATGCCTGGAGGAACAGAATCTGAAAGAACTCGACAAGCTGCTTCAGAGAAGCCTGAAAGCATTGTGG ACAGGGTTCGACGAACGGACTGTGGCATCAGATCTCGCAGACATAATATTTCCGGAGAGGCTCCGGGTTA CTTTGAGGAATGGTCTGCCCGATTTCATGTCCCAGTCAATGCTGAACAACTTCCGATCCTTCATTCTGGA AAGGTCCGGAATACTGCCCGCTACCTGCTGTGCGTTGCCAAGTGACTTTGTTCCTTTGACATACCGGGAA TGTCCGCCGCCCCTGTGGTCACACTGTTATCTGTTTCGGCTTGCAAACTACCTGAGCTACCATTCTGACG TCATTGAGGATGTCAGCGGAGATGGCCTCCTTGAGTGCCATTGTAGGTGCAATCTTTGCACTCCCCACCG CTCATTGGCGTGCAATCCCCAACTGTTGTCCGAAACACAGATTATTGGCACCTTCGAGCTGCAGGGGCCT TCTTCCGAGGGAGAGGGGAGCAGTCCCGGTCAAAGTCTTAAGCTTACTCCTGGACTGTGGACATCAGCCT ATCTCAGAAAATTCCATCCTGAGGATTACCATCCATATGAGATACGCTTTTACGAGGATCAGTCTCACCC ACCAAAGGTAGAGCTCAGCGCGTGTGTGATAACTCAGGGAGCAATACTGGCTCAATTGCAGGCTATCCAG AAATCCCGACAGGAGTTCCTGCTCAAAAAGGGCAGCGGCGTGTACTTGGACCCTCAGACAGGAGAGGAAT TGAACACCAGGTTTCCCCAAGACGTGCCGTCTCCTCGAAAGCAAGAAGCCGAAGGCGCAGCGGATGCTCC AAGAGGGTACGGTGGGAGGCTGGGCCAGTCCGGAAGGGGTGGTGATGGCAGACTTGGACAGCCAGGGAGA GGCGGAGGCCAGCCGGGAGGACGACAATTTGGTGGTGGCCGCAGAGGAGGGAGAGGTGGCGGCAGAAGCA ATAGACGCCAGACCGTGGTGATTGGTGGCGGCGACAAGCAAGTGCCACGACAGCAGCACGGCTACCACTT GCGCTCAGGCTCCGGAGGACCCGCTGCTGCCCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100195 representing AP_000551
Red=Cloning sites Green=Tags METQPSLPTSLQAPSHLAPSSDEEESLTTPPPSPATTTSTLDDEEEEVDAAQEMQAQDNVKAEEIEADVE QDPGYVTPAEHEEELKRFIDREDDDRPEHQADGDHQEAGIGDQVADYLTGLGGEDVLLKHLARQSNIVKD ALLDLTEVPISVEELSRAYELNLFSPHIPPKRQPNGTCEANPRLNFYPAFTVPEVLATYHIFFKNQKIPV SCRANRTRADALLNLGPGARLPDIASLEEVPKIFEGLGSDETRAANALQQGENGMDEHHSALVELEGDNA RLAVLKRSIVVTHFAYPAVNLPPKVMSAVMDHLLIKRAGPLSENQNMQDPDASDEGKPVVSDEQLSRWLG TNSPRDLEERRKLMMAVVLVTVELECLRRFFTDPETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSY MGILHENRLGQSVLHTTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQRSLKALW TGFDERTVASDLADIIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRE CPPPLWSHCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLLSETQIIGTFELQGP SSEGEGSSPGQSLKLTPGLWTSAYLRKFHPEDYHPYEIRFYEDQSHPPKVELSACVITQGAILAQLQAIQ KSRQEFLLKKGSGVYLDPQTGEELNTRFPQDVPSPRKQEAEGAADAPRGYGGRLGQSGRGGDGRLGQPGR GGGQPGGRQFGGGRRGGRGGGRSNRRQTVVIGGGDKQVPRQQHGYHLRSGSGGPAAAQQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | AC_000018 |
ORF Size | 2487 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | AC_000018.1, AP_000551 |
RefSeq ORF | 2487 bp |
MW | 92.0 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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VC100190 | Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus 7], codon optimized for human cell expression, AP_000546 |
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VC100191 | Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 7], codon optimized for human cell expression, AP_000547 |
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VC100192 | Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 7], codon optimized for human cell expression, AP_000548 |
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VC100193 | Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 7], codon optimized for human cell expression, AP_000549 |
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VC100194 | Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 7], codon optimized for human cell expression, AP_000550 |
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VC100196 | Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 7], codon optimized for human cell expression, AP_000553 |
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VC100197 | Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 7], codon optimized for human cell expression, AP_000552 |
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VC100198 | Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 7], codon optimized for human cell expression, AP_000554 |
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VC100199 | Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus 7], codon optimized for human cell expression, AP_000555 |
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VC100200 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha0 [Human adenovirus 7], codon optimized for human cell expression, AP_000556 |
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VC100201 | Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 7], codon optimized for human cell expression, AP_000557 |
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VC100202 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus 7], codon optimized for human cell expression, AP_000558 |
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VC100203 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-gamma1 [Human adenovirus 7], codon optimized for human cell expression, AP_000559 |
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VC100204 | Myc-DDK-tagged ORF clone of viral ORF for E3 RID-alpha [Human adenovirus 7], codon optimized for human cell expression, AP_000560 |
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VC100205 | Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 7], codon optimized for human cell expression, AP_000561 |
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VC100206 | Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 7], codon optimized for human cell expression, AP_000562 |
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VC100207 | Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 7], codon optimized for human cell expression, AP_000564 |
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VC100208 | Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 7], codon optimized for human cell expression, AP_000566 |
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VC100209 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 7], codon optimized for human cell expression, AP_000567 |
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VC100210 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus 7], codon optimized for human cell expression, AP_000568 |
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VC100211 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus 7], codon optimized for human cell expression, AP_000569 |
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VC100212 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus 7], codon optimized for human cell expression, AP_000570 |
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VC100213 | Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 7], codon optimized for human cell expression, AP_000538 |
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VC100214 | Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 7], codon optimized for human cell expression, AP_000539 |
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VC100215 | Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 7], codon optimized for human cell expression, AP_000540 |
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VC100216 | Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 7], codon optimized for human cell expression, AP_000545 |
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VC100217 | Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus 7], codon optimized for human cell expression, AP_000563 |
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VC100218 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 7], codon optimized for human cell expression, AP_000565 |
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