100K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100195

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 7], codon optimized for human cell expression, AP_000551



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CNY 12,540.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100195 represents NCBI reference of AP_000551 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACCCAACCATCCTTGCCCACAAGCCTGCAGGCGCCTTCACATCTGGCCCCATCATCCGATGAGG
AAGAGTCACTGACCACACCGCCGCCTAGCCCTGCGACAACCACGAGTACGCTGGACGACGAGGAGGAGGA
GGTTGACGCCGCACAGGAGATGCAGGCTCAGGATAATGTAAAGGCAGAGGAGATAGAGGCTGATGTGGAG
CAGGATCCCGGATACGTGACACCTGCTGAACATGAAGAAGAATTGAAGCGCTTTATCGATCGCGAAGACG
ATGACAGGCCCGAGCACCAGGCTGACGGCGACCACCAGGAGGCTGGGATTGGCGACCAAGTGGCAGACTA
CCTTACGGGGCTGGGGGGCGAGGACGTTCTCCTGAAGCACCTTGCCCGCCAGTCTAATATCGTCAAGGAC
GCCCTGCTCGATTTGACGGAAGTACCTATATCCGTGGAAGAACTTTCCAGAGCTTACGAATTGAACCTCT
TCTCCCCCCACATCCCGCCAAAGCGCCAGCCCAATGGTACTTGCGAAGCTAACCCCCGGCTGAATTTTTA
TCCCGCATTCACCGTGCCAGAAGTCCTGGCGACCTATCACATCTTTTTTAAAAACCAGAAAATACCCGTC
AGCTGTAGGGCCAATCGGACACGCGCCGACGCCCTCCTTAATCTCGGACCCGGTGCGAGACTGCCCGACA
TCGCTTCCCTCGAAGAAGTCCCTAAGATCTTCGAGGGGCTCGGCTCCGATGAGACCCGGGCAGCCAATGC
TCTGCAGCAAGGGGAAAATGGAATGGATGAGCACCACTCAGCCCTCGTCGAACTCGAAGGTGATAACGCA
AGACTCGCGGTCCTGAAGCGGTCTATAGTCGTGACCCATTTCGCTTATCCCGCTGTGAATCTGCCACCTA
AGGTGATGTCTGCCGTAATGGACCACCTTTTGATAAAGCGAGCAGGCCCACTGAGCGAAAACCAAAACAT
GCAGGACCCAGATGCTTCAGACGAGGGAAAACCCGTCGTCAGCGATGAACAACTGTCTCGATGGCTCGGC
ACTAACAGCCCCCGGGACCTGGAGGAGCGACGCAAGCTGATGATGGCCGTTGTGCTGGTGACTGTAGAAT
TGGAATGTCTGCGCCGCTTCTTCACTGACCCTGAAACCCTGCGGAAGCTGGAGGAGAACCTTCATTACAC
ATTTAGACACGGCTTCGTGCGCCAGGCATGCAAAATCAGCAACGTAGAACTGACCAACTTGGTCAGCTAC
ATGGGGATCCTGCACGAGAATAGACTGGGACAGAGTGTTCTGCACACCACCCTCAAGGGAGAAGCGCGCA
GGGATTACATTAGGGACTGTGTGTACCTCTACCTGTGCCATACCTGGCAAACCGGGATGGGAGTATGGCA
GCAATGCCTGGAGGAACAGAATCTGAAAGAACTCGACAAGCTGCTTCAGAGAAGCCTGAAAGCATTGTGG
ACAGGGTTCGACGAACGGACTGTGGCATCAGATCTCGCAGACATAATATTTCCGGAGAGGCTCCGGGTTA
CTTTGAGGAATGGTCTGCCCGATTTCATGTCCCAGTCAATGCTGAACAACTTCCGATCCTTCATTCTGGA
AAGGTCCGGAATACTGCCCGCTACCTGCTGTGCGTTGCCAAGTGACTTTGTTCCTTTGACATACCGGGAA
TGTCCGCCGCCCCTGTGGTCACACTGTTATCTGTTTCGGCTTGCAAACTACCTGAGCTACCATTCTGACG
TCATTGAGGATGTCAGCGGAGATGGCCTCCTTGAGTGCCATTGTAGGTGCAATCTTTGCACTCCCCACCG
CTCATTGGCGTGCAATCCCCAACTGTTGTCCGAAACACAGATTATTGGCACCTTCGAGCTGCAGGGGCCT
TCTTCCGAGGGAGAGGGGAGCAGTCCCGGTCAAAGTCTTAAGCTTACTCCTGGACTGTGGACATCAGCCT
ATCTCAGAAAATTCCATCCTGAGGATTACCATCCATATGAGATACGCTTTTACGAGGATCAGTCTCACCC
ACCAAAGGTAGAGCTCAGCGCGTGTGTGATAACTCAGGGAGCAATACTGGCTCAATTGCAGGCTATCCAG
AAATCCCGACAGGAGTTCCTGCTCAAAAAGGGCAGCGGCGTGTACTTGGACCCTCAGACAGGAGAGGAAT
TGAACACCAGGTTTCCCCAAGACGTGCCGTCTCCTCGAAAGCAAGAAGCCGAAGGCGCAGCGGATGCTCC
AAGAGGGTACGGTGGGAGGCTGGGCCAGTCCGGAAGGGGTGGTGATGGCAGACTTGGACAGCCAGGGAGA
GGCGGAGGCCAGCCGGGAGGACGACAATTTGGTGGTGGCCGCAGAGGAGGGAGAGGTGGCGGCAGAAGCA
ATAGACGCCAGACCGTGGTGATTGGTGGCGGCGACAAGCAAGTGCCACGACAGCAGCACGGCTACCACTT
GCGCTCAGGCTCCGGAGGACCCGCTGCTGCCCAGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100195 representing AP_000551
Red=Cloning sites Green=Tags

METQPSLPTSLQAPSHLAPSSDEEESLTTPPPSPATTTSTLDDEEEEVDAAQEMQAQDNVKAEEIEADVE
QDPGYVTPAEHEEELKRFIDREDDDRPEHQADGDHQEAGIGDQVADYLTGLGGEDVLLKHLARQSNIVKD
ALLDLTEVPISVEELSRAYELNLFSPHIPPKRQPNGTCEANPRLNFYPAFTVPEVLATYHIFFKNQKIPV
SCRANRTRADALLNLGPGARLPDIASLEEVPKIFEGLGSDETRAANALQQGENGMDEHHSALVELEGDNA
RLAVLKRSIVVTHFAYPAVNLPPKVMSAVMDHLLIKRAGPLSENQNMQDPDASDEGKPVVSDEQLSRWLG
TNSPRDLEERRKLMMAVVLVTVELECLRRFFTDPETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSY
MGILHENRLGQSVLHTTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQRSLKALW
TGFDERTVASDLADIIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRE
CPPPLWSHCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLLSETQIIGTFELQGP
SSEGEGSSPGQSLKLTPGLWTSAYLRKFHPEDYHPYEIRFYEDQSHPPKVELSACVITQGAILAQLQAIQ
KSRQEFLLKKGSGVYLDPQTGEELNTRFPQDVPSPRKQEAEGAADAPRGYGGRLGQSGRGGDGRLGQPGR
GGGQPGGRQFGGGRRGGRGGGRSNRRQTVVIGGGDKQVPRQQHGYHLRSGSGGPAAAQQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000018
ORF Size 2487 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000018.1, AP_000551
RefSeq ORF 2487 bp
MW 92.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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