pol (AC_000018) Virus Tagged ORF Clone

CAT＃: VC100214

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 7], codon optimized for human cell expression, AP_000539

Datasheet

SDS

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CNY 18,050.00

货期*

6周

规格

Product images

经常一起买 (4)

PS100001

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TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

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【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100214 represents NCBI reference of AP_000539 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGTACAGGCACACGGCGCTGGTAGCTTCCATCCCGAAGCCTCTGATCCGGGCTGCCAGCCCC CTCGGAGAAGGACTTGCGAATCCAGTCAAGGGGCAGTGCCGGAGCCAACAAGGGCACGAAGGCGACGCAC TACAGCATCTCATGCCGCAGGGTCTAGGGCGGCGCCTGCCGCAAGGTGTGCGCCTAGCACTCCCCTGCTC AATATGCAAGAGGCTACCGAGCCCCCACCTCCAAAGAGGAAGAACAAGGGAACAGTAGTGGCCCCAAAGG GCCATGGGACTCTCCAGGCAATAGATATCAGTACTAACGGGCCAGTCGAGATTAAGTATCATCTTAATTT GCCTCACGCTCTGGAGAAAATCATGCAGGTGAACCTCCTCACACTGCCAACAAATCTGACCCCACAGCGG CTGAGAACTCTGGACTCATCAGGACTGCGGGCTCTGGTGCTGGAGCTGCGCCCATGCCGCGCCGAAGTTT GGACATGTCTCCCCAGGGGCCTGGTGAGCATGACTACGATTGAGACAGAGGATGGACATGCAGACGCTGA TAATATTGTCGAACGCCAGGTTCAGACCCCTGGGCTCAATTTTCCACTGAAGTTCCTGGTTAAGGGAAGC CAGGTCCAGTTGATCCACGAAGTGCACCCGGTTAACCGCTGTGAGTACTGCGGGCGGCTCTACAAACATA AGCACGAATGTTCCGCCCGACGCAGAGAGTTTTACTTCCACCACATCAACTCTCATAGCTCAAATTGGTG GCAGGAAATACAATTCTTTCCTATCGGATCACATCCCCGCACTGAGCGACTGTTCCTCACTTACGACGTG GAGACATATACTTGGATGGGTAGTTTTGGCAAACAGCTCATCCCTTTCATGCTGGTGATGAAACTCAGCG GCGACCAGAGGCTCGTGAACATCGCCTATGATCTGGCAATGAAACTGAAGTGGGACCGCTGGAGACAGGA TCCCCAGACATTCTACTGCGTGACTCCTGAAAAAATGGCAGTCGGCCAGCACTTCAGGCAATACCGAGAT CAATTGCAGACAGCGCTGGCGGTAGACCTGTGGTCCTCATTTCTCAAGGCTAACCCCCACGTGCATGAGT GGGCTCTGGAACACTACGCACTGACCGATCCTACGGATCTCACTTTCGAAGAATTGAAAAAACTGCCGCA TGTGAGAGGGACACCCCGGTTCCTGGAACTTTATATAGTCGGGCATAACATCAACGGCTTCGATGAAATA GTCCTGGCCGCACAGGTTATCAATAATCGCGCAGAAGTCCCCCAGCCCTTTAGAATCACCAGGAATTTTA TGCCCAGAGCTGGAAAAATACTGTTTAATGACGTTACATTCGCCTTGCCGAATCCAGCCTACAAGAAAAG AGTAGATTTTCAACTCTGGGAGCAGGGGGCATGTGACGACATGGATTTTAAGTACCAGTTTTTGAAAGTG ATGGTGCGGGACACCTTTGCTCTCACACATACCAGCCTGCGGAACGCCGCACAGGCATACTCACTTCCTG TGGAGAAAGGATGTTGCCCTTACAAAGCTGTAAATCAGTTCTACATGCTCGGGTCTTATCGAGCAGAGAA GGATGGCTTCCCGCTGGAGGAGTATTGGAAAGATCGAGAAGAGTATCTTCTGAACAGAGAGCTCTGGGAG AAAAAGTCTCAACCTCGATACGACATCATTCAGGAAACACTGGATTATTGCGCCCTTGATGTTCTGGTTA CGGCTGAGCTCGTGGCAAAGCTCCAAGAATCATACGCACACTTCATCCGCGACTCTGTAGGCCTGCCACA CGCACACTTTAATATATTTCAAAGACCTACAATTAGCAGCAATTCTCACGCCATTTTCAGACAGATCGTG TATAGAGCAGAGAAGCCTAATCGAACCAACCTGGGTCCTGGCCTCCTCGCACCTTCTCACGAACTGTACG ACTACGTGAGGGCTAGCATTCGAGGTGGACGCTGCTATCCGACATATATCGGCATCCTGGAAGAGCCTCT GTACGTCTACGACATCTGTGGCATGTATGCAAGTGCGCTGACGCACCCCATGCCATGGGGAACTCCTCTC AACCCGTACGAGCGAGCTCTGGCAGTTAGGGAGTGGCAGATGACCCTCGACGATCCCACAACAATTAGTT ACTTTGATAAAGACCTCCTGCCCGGGATCTTTACTATCGACGCTGATCCCCCTGATGAGCTTATGCTTGA CCCTCTCCCACCATTTTGTAGTCGGAAGGGGGGCCGCTTGTGTTGGACAAATGAGCCTCTCCGCGGCGAG GTGGCCACTAGCGTTGACCTGATCACCTTGCACAACCGGGGCTGGAGGGTTCGGATTGTGCCTGACGAGC TCACCACAATCTTTCCTGAGTGGAAGTGCGTTGCTCGAGAGTACGTGCAGCTCAATATCGCTGCCAAGGA GCGGGCCGACAAAGAGAAAAACCAGACCATGAGAAGCATCGCCAAACTGCTTAGCAACGCCCTGTACGGC TCCTTTGCCACGAAACTTGACAACAAAAAAATCGTATTTAGCGATCAGATGGATGAAAGTTTGATGAAAG GAATCTCCGCGGGCACCGTTAACATTAAGTCCAGTTCCTTTCTCGAAACGGACAATCTCTCTGCCGAGGT CATGCCCGCTTTTGAGAGAGAATATCTGCCACAGCAACTTGCCCTCCTCGACTCCGATCCTGAGGACTCA GAAGATGAACAGCGGCCCGCACCCTTTTACACACCACCCGCCGGTACCCCAGGCCACGTTGCTTATACCT ACAAACCTATCACTTTCCTCGATGTTGAAGAGGGGGATATGTGTCTGCATACCGTAGAGAAGGTGGACCC CTTGGTGGATAACGATAGATACCCTTCTCACGTAGCTTCCTTTGTACTTGCCTGGACCAGAGCATTTGTG TCCGAGTGGGCCGGATTCCTGTACGAAGAGGATAGGGGCACACCGCTGGAGGATAGACCCATCAAAAGCG TATACGGTGACACCGATTCTTTGTTTGTCACTCAACGGGGACACGACTTGATGGAGACAAAGGGTAAGAA AAGAATCAAGAAGCACGGCGGGAAGCTCGTGTTCGATCCTGATGAACCGGACCTGACCTGGCTGGTGGAA TGTGAGACAGTGTGCGCTAGTTGTGGTGCTGATGCATACAGCCCGGAAAGTATCTTCCTCGCACCCAAGT TGTACGCCCTGAAGTGCATATACTGTCCAGCATGTCACAAGACTTCTAAGGGAAAACTTCGGGCAAAGGG CCATGCCGCCGAGGCCCTGAACTACGAACTGATGGTGAACTGCTATCTCGCGGATATGCAAGGAGCAGAT CGGCAGCGCTTTTCTACATCCAGAATGAGTCTGAAGCGGACACTCGCCTCCGCTCAGCCCGGTGCACACC CCTTTACAGTGACCGAGACAACCCTGACACGCACGCTGCGCCCCTGGAAGGACCGCACACTGGCGGCTCT GGACGCCCACAGGCTGATACCATACAGCAGAAGCCGACCAAATCCTCGCAATGAAGAAGTCTGCTGGATC GAGATGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100214 representing AP_000539 Red=Cloning sites Green=Tags MALVQAHGAGSFHPEASDPGCQPPRRRTCESSQGAVPEPTRARRRRTTASHAAGSRAAPAARCAPSTPLL NMQEATEPPPPKRKNKGTVVAPKGHGTLQAIDISTNGPVEIKYHLNLPHALEKIMQVNLLTLPTNLTPQR LRTLDSSGLRALVLELRPCRAEVWTCLPRGLVSMTTIETEDGHADADNIVERQVQTPGLNFPLKFLVKGS QVQLIHEVHPVNRCEYCGRLYKHKHECSARRREFYFHHINSHSSNWWQEIQFFPIGSHPRTERLFLTYDV ETYTWMGSFGKQLIPFMLVMKLSGDQRLVNIAYDLAMKLKWDRWRQDPQTFYCVTPEKMAVGQHFRQYRD QLQTALAVDLWSSFLKANPHVHEWALEHYALTDPTDLTFEELKKLPHVRGTPRFLELYIVGHNINGFDEI VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDMDFKYQFLKV MVRDTFALTHTSLRNAAQAYSLPVEKGCCPYKAVNQFYMLGSYRAEKDGFPLEEYWKDREEYLLNRELWE KKSQPRYDIIQETLDYCALDVLVTAELVAKLQESYAHFIRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV YRAEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGILEEPLYVYDICGMYASALTHPMPWGTPL NPYERALAVREWQMTLDDPTTISYFDKDLLPGIFTIDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE VATSVDLITLHNRGWRVRIVPDELTTIFPEWKCVAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG SFATKLDNKKIVFSDQMDESLMKGISAGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLALLDSDPEDS EDEQRPAPFYTPPAGTPGHVAYTYKPITFLDVEEGDMCLHTVEKVDPLVDNDRYPSHVASFVLAWTRAFV SEWAGFLYEEDRGTPLEDRPIKSVYGDTDSLFVTQRGHDLMETKGKKRIKKHGGKLVFDPDEPDLTWLVE CETVCASCGADAYSPESIFLAPKLYALKCIYCPACHKTSKGKLRAKGHAAEALNYELMVNCYLADMQGAD RQRFSTSRMSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI EMP TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	AC_000018
ORF Size	3579 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AC_000018.1, AP_000539
RefSeq ORF	3579 bp
MW	136.0 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100182	Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 7], codon optimized for human cell expression, AP_000534	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100183	Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 7], codon optimized for human cell expression, AP_000535	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100184	Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 7], codon optimized for human cell expression, AP_000536	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,990.00
VC100185	Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus 7], codon optimized for human cell expression, AP_000537	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100186	Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 7], codon optimized for human cell expression, AP_000543	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,560.00
VC100187	Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 7], codon optimized for human cell expression, AP_000541	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,660.00
VC100188	Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 7], codon optimized for human cell expression, AP_000542	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,130.00
VC100189	Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 7], codon optimized for human cell expression, AP_000544	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100190	Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus 7], codon optimized for human cell expression, AP_000546	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100191	Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 7], codon optimized for human cell expression, AP_000547	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100192	Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 7], codon optimized for human cell expression, AP_000548	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,060.00
VC100193	Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 7], codon optimized for human cell expression, AP_000549	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100194	Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 7], codon optimized for human cell expression, AP_000550	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,270.00
VC100195	Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 7], codon optimized for human cell expression, AP_000551	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,540.00
VC100196	Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 7], codon optimized for human cell expression, AP_000553	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100197	Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 7], codon optimized for human cell expression, AP_000552	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100198	Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 7], codon optimized for human cell expression, AP_000554	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100199	Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus 7], codon optimized for human cell expression, AP_000555	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100200	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha0 [Human adenovirus 7], codon optimized for human cell expression, AP_000556	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100201	Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 7], codon optimized for human cell expression, AP_000557	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100202	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus 7], codon optimized for human cell expression, AP_000558	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100203	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-gamma1 [Human adenovirus 7], codon optimized for human cell expression, AP_000559	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100204	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-alpha [Human adenovirus 7], codon optimized for human cell expression, AP_000560	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100205	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 7], codon optimized for human cell expression, AP_000561	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100206	Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 7], codon optimized for human cell expression, AP_000562	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100207	Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 7], codon optimized for human cell expression, AP_000564	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100208	Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 7], codon optimized for human cell expression, AP_000566	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100209	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 7], codon optimized for human cell expression, AP_000567	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100210	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus 7], codon optimized for human cell expression, AP_000568	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100211	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus 7], codon optimized for human cell expression, AP_000569	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100212	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus 7], codon optimized for human cell expression, AP_000570	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100213	Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 7], codon optimized for human cell expression, AP_000538	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,510.00
VC100215	Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 7], codon optimized for human cell expression, AP_000540	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,890.00
VC100216	Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 7], codon optimized for human cell expression, AP_000545	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,180.00
VC100217	Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus 7], codon optimized for human cell expression, AP_000563	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100218	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 7], codon optimized for human cell expression, AP_000565	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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