L3 (NC_001460) Virus Tagged ORF Clone
CAT#: VC100268
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040924
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CNY 13,870.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100268 represents NCBI reference of NP_040924 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTCCAAGCATGATGCCACAGTGGAGTTACATGCACATCGCCGGACAGGATGCGAGCGAGTATT TGAGTCCAGGTCTCGTTCAGTTCGCGAGAGCCACTGATACTTATTTTACCCTCGGGAACAAATTTAGGAA TCCAACAGTAGCTCCAACACACGACGTGACCACTGACCGCTCCCAGAGGCTTACCCTCCGGTTCGTGCCC GTCGATCGGGAAGATACAACATATTCCTATAAAGCCCGCTTCACTCTCGCCGTCGGAGACAACAGGGTAC TGGATATGGCTTCAAGCTACTTCGACATCAGGGGCGTGCTGGATAGAGGCCCGTCATTTAAACCTTACAG CGGTACAGCGTATAATTCTCTGGCCCCCAAGGGCGCTCCGAACGCATCTCAATGGAGTGATAACGCAAAG CTCAACACCTTCGCACAGGCTCCATACCTCAGTGATACCATCACAGCCGCCGATGGGATTAAGGTCGGTA CCGACACTGCCCAGGCCGGAGCCGCGGTATATGCTAATAAGACTTATCAACCAGAGCCACAGGTAGGCCC TTCTGAGTGGAACACGAGCATTGAGAACGTTAAGGCGGGCGGCCGAGCTTTGAAGCAAACAACCGCAATG CAACCTTGCTATGGTTCTTACGCACGCCCGACTAATGAACATGGCGGCCAGTCCAAAGACGATAATATTG AACTGAAGTTCTTTGACTCTGCCAACAACGCAGCGAACACTGCGCAGGTTGTTTTCTATACCGAAGATGT GAACCTCGAAATGCCGGATACGCACCTGGTGTTTAAACCAACCGTCACTAACGGGACCATCGCCAGTGAG TCTCTGTTGGGCCAGCAGGCTGCCCCAAACCGCGCAAACTACATCGCTTTCAGAGACAACTTTATCGGGC TTATGTACTACAATTCTACTGGGAACATGGGAGTGCTTGCTGGGCAGGCATCTCAGCTGAATGCAGTAGT GGATCTCCAAGATCGCAATACCGAGCTCTCTTATCAACTCATGCTGGATGCGCTGGGGGATCGGACCCGC TACTTTAGCCTGTGGAACAGCGCCGTGGATTCATACGACCCGGATGTCCGGGTGATCGAGAACCATGGAG TCGAAGACGAATTGCCCAACTACTGTTTTCCACTGAGCGCGGTGGGAGAGATTAAAAACTATAAGGGGAT CAAACCCGACAACGGCGGCGGTGGCGGGTGGACAGCCGATAACACAGTTAGCGAGGCCAACCATATTGGA ATTGGCAATATTGCCGCAATGGAGATTAACCTTCAGGCCAACCTGTGGCGCAGTTTCCTGTACTCCAACG TTGGCCTTTACCTGCCCGATGATCTGAAATACACGCCAGGTAACATAAAACTCCCCGATAACAAGAACAC GTACGAGTATATGAATGGCCGGGTAACAGCTCCCGGGTTGGTGGACACTTACGTGAATATAGGCGCCCGC TGGAGCCCTGACGTGATGGACAACGTTAATCCCTTCAATCATCATCGGAACGCGGGCCTGCGCTATCGGA GTATGCTGTTGGGCAATGGAAGGTTCGTCCCTTTTCATATCCAAGTGCCCCAGAAGTTTTTCGCAATTCG GAACCTGCTCCTCCTTCCAGGCAGTTACACATACGAGTGGAACTTCAGAAAGGATGTGAATATGATTCTT CAATCTACTCTGGGCAATGACCTGAGAGTGGACGGCGCCTCTGTGCGGTTTGATAACATCGCGCTGTATG CTAATTTTTTCCCTATGGCTCACAACACAGCCTCCACGTTGGAAGCTATGCTGCGCAACGATACCAACGA TCAGTCTTTCAACGATTATCTCTGCGCAGCAAACATGCTCTATCCAATCCCCGCCAACGCAACCTCCGTT CCGATCTCAATTCCCTCCCGGAACTGGGCAGCCTTTAGAGGCTGGAGTTTCACGCGACTTAAGACGAAAG AAACGCCTTCTCTTGGTTCCGGGTTCGATCCATACTTTGTGTATTCCGGTACAATCCCGTATCTGGACGG AACCTTCTACCTTAACCACACTTTCAAGAAAGTTTCAATCATGTTCGACTCATCCGTTTCCTGGCCGGGG AATGATCGGCTGCTGACTCCCAATGAATTTGAGATCAAACGCTCTGTCGACGGAGAAGGCTATAACGTGG CACAATGCAATATGACCAAAGACTGGTTCCTGATTCAGATGCTCTCTCATTATAACATCGGCTATCAGGG CTTCTATATACCAGAATCTTACAAAGATCGGATGTACTCATTTTTTCGGAACTTTCAGCCCATGAGTCGA CAGGTGGTCGATACCACAGAGTATAAGAACTATAAAAAGGTGACAGTGGAGTTCCAGCACAATAATAGCG GGTTCGTGGGATATCTGGGGCCAACGATGAGAGAGGGCCAGGCCTATCCCGCCAATTATCCTTATCCTCT GATAGGTCAGACCGCTGTTGAGTCCATCACCCAGAAAAAATTCCTGTGCGATAGGGTCATGTGGCGGATC CCTTTCTCATCAAACTTTATGTCTATGGGAGCTCTCACTGATCTCGGACAAAACATGCTCTATGCAAATT CCGCACACGCCCTTGATATGACCTTTGAGGTTGACCCTATGGATGAACCCACCCTTCTCTATGTTCTCTT CGAGGTCTTTGATGTCGTGCGGATTCACCAGCCACACCGCGGCGTCATAGAGGCTGTGTACCTGCGCACC CCATTCAGCGCTGGCAACGCTACAACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100268 representing NP_040924
Red=Cloning sites Green=Tags MATPSMMPQWSYMHIAGQDASEYLSPGLVQFARATDTYFTLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP VDREDTTYSYKARFTLAVGDNRVLDMASSYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNASQWSDNAK LNTFAQAPYLSDTITAADGIKVGTDTAQAGAAVYANKTYQPEPQVGPSEWNTSIENVKAGGRALKQTTAM QPCYGSYARPTNEHGGQSKDDNIELKFFDSANNAANTAQVVFYTEDVNLEMPDTHLVFKPTVTNGTIASE SLLGQQAAPNRANYIAFRDNFIGLMYYNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLMLDALGDRTR YFSLWNSAVDSYDPDVRVIENHGVEDELPNYCFPLSAVGEIKNYKGIKPDNGGGGGWTADNTVSEANHIG IGNIAAMEINLQANLWRSFLYSNVGLYLPDDLKYTPGNIKLPDNKNTYEYMNGRVTAPGLVDTYVNIGAR WSPDVMDNVNPFNHHRNAGLRYRSMLLGNGRFVPFHIQVPQKFFAIRNLLLLPGSYTYEWNFRKDVNMIL QSTLGNDLRVDGASVRFDNIALYANFFPMAHNTASTLEAMLRNDTNDQSFNDYLCAANMLYPIPANATSV PISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGTIPYLDGTFYLNHTFKKVSIMFDSSVSWPG NDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQMLSHYNIGYQGFYIPESYKDRMYSFFRNFQPMSR QVVDTTEYKNYKKVTVEFQHNNSGFVGYLGPTMREGQAYPANYPYPLIGQTAVESITQKKFLCDRVMWRI PFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRIHQPHRGVIEAVYLRT PFSAGNATT TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001460 |
ORF Size | 2757 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001460.1, NP_040924 |
RefSeq ORF | 2757 bp |
Locus ID | 1460858 |
MW | 103.0 kDa |
Documents
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SDS |
Resources
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VC100275 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040932 |
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VC100277 | Myc-DDK-tagged ORF clone of viral ORF for L5 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040933 |
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VC100278 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040934 |
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VC100279 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040935 |
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VC100280 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040936 |
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VC100281 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597783 |
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VC100282 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597784 |
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